Restriction map for
pF1KB3608
(Linear) (Six-base) MAPSORT of: KIBB3608.seq Check: 1645 from: 1 to: 630
KIBB3608.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AfeI AGC'GCT
Cuts at: 0 292 630
Size: 292 338
AleI CACnn'nnGTG
Cuts at: 0 211 340 630
Size: 211 129 290
Fragments arranged by size:
290 211 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 141 173 630
Size: 141 32 457
Fragments arranged by size:
457 141 32
ApoI r'AATT_y
Cuts at: 0 580 630
Size: 580 50
AvaI C'yCGr_G
Cuts at: 0 483 524 630
Size: 483 41 106
Fragments arranged by size:
483 106 41
BanI G'GyrC_C
Cuts at: 0 230 630
Size: 230 400
BbeI G_GCGC'C
Cuts at: 0 234 630
Size: 234 396
BbsI GAAGACnn'nnnn_
Cuts at: 0 431 630
Size: 431 199
BglII A'GATC_T
Cuts at: 0 221 630
Size: 221 409
BlpI GC'TnA_GC
Cuts at: 0 173 630
Size: 173 457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 152 630
Size: 152 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 145 630
Size: 134 11 485
Fragments arranged by size:
485 134 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 50 610 630
Size: 50 560 20
Fragments arranged by size:
560 50 20
BsaHI Gr'CG_yC
Cuts at: 0 231 630
Size: 231 399
BsaWI w'CCGG_w
Cuts at: 0 575 630
Size: 575 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 141 171 630
Size: 141 30 459
Fragments arranged by size:
459 141 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 307 343 364 446 630
Size: 34 273 36 21 82 184
Fragments arranged by size:
273 184 82 36 34 21
BsrBI CCG'CTC
Cuts at: 0 23 104 630
Size: 23 81 526
Fragments arranged by size:
526 81 23
BsrDI GCAATG_nn'
Cuts at: 0 167 630
Size: 167 463
BsrFI r'CCGG_y
Cuts at: 0 177 554 630
Size: 177 377 76
Fragments arranged by size:
377 177 76
BssSI C'ACGA_G
Cuts at: 0 137 630
Size: 137 493
BstEII G'GTnAC_C
Cuts at: 0 528 630
Size: 528 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 261 630
Size: 261 369
BstYI r'GATC_y
Cuts at: 0 221 630
Size: 221 409
BtgI C'CryG_G
Cuts at: 0 410 630
Size: 410 220
BtsI GCAGTG_nn'
Cuts at: 0 331 630
Size: 331 299
EaeI y'GGCC_r
Cuts at: 0 179 268 630
Size: 179 89 362
Fragments arranged by size:
362 179 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 541 630
Size: 541 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 145 630
Size: 134 11 485
Fragments arranged by size:
485 134 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 414 630
Size: 414 216
HaeII r_GCGC'y
Cuts at: 0 234 294 630
Size: 234 60 336
Fragments arranged by size:
336 234 60
KasI G'GCGC_C
Cuts at: 0 230 630
Size: 230 400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 65 630
Size: 63 2 565
Fragments arranged by size:
565 63 2
MslI CAynn'nnrTG
Cuts at: 0 211 340 441 593 630
Size: 211 129 101 152 37
Fragments arranged by size:
211 152 129 101 37
MspA1I CmG'CkG
Cuts at: 0 348 460 630
Size: 348 112 170
Fragments arranged by size:
348 170 112
NaeI GCC'GGC
Cuts at: 0 179 556 630
Size: 179 377 74
Fragments arranged by size:
377 179 74
NarI GG'CG_CC
Cuts at: 0 231 630
Size: 231 399
NgoMIV G'CCGG_C
Cuts at: 0 177 554 630
Size: 177 377 76
Fragments arranged by size:
377 177 76
NspI r_CATG'y
Cuts at: 0 440 630
Size: 440 190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 630
Size: 141 32 457
Fragments arranged by size:
457 141 32
PvuII CAG'CTG
Cuts at: 0 348 460 630
Size: 348 112 170
Fragments arranged by size:
348 170 112
SfoI GGC'GCC
Cuts at: 0 232 630
Size: 232 398
SmaI CCC'GGG
Cuts at: 0 485 630
Size: 485 145
SphI G_CATG'C
Cuts at: 0 440 630
Size: 440 190
StyI C'CwwG_G
Cuts at: 0 563 621 630
Size: 563 58 9
Fragments arranged by size:
563 58 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 630
Size: 352 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 417 630
Size: 417 213
XmaI C'CCGG_G
Cuts at: 0 483 630
Size: 483 147
Enzymes that do cut:
AfeI AleI AloI ApoI AvaI BanI BbeI BbsI
BglII BlpI BplI BpmI BsaI BsaHI BsaWI BsaXI
BsgI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI
BtgI BtsI EaeI EciI Eco57MI FalI HaeII KasI
MmeI MslI MspA1I NaeI NarI NgoMIV NspI PpiI
PvuII SfoI SmaI SphI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbvCI BcgI BciVI
BclI BfrBI BglI Bme1580I BmgBI BmrI BmtI Bpu10I
BpuEI BsaAI BsaBI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrGI BssHII
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MscI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmnI ZraI