Restriction map for
pF1KB3604
(Linear) (Six-base) MAPSORT of: KIBB3604.seq Check: 6774 from: 1 to: 1500
KIBB3604.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 104 1500
Size: 104 1396
Acc65I G'GTAC_C
Cuts at: 0 533 1274 1500
Size: 533 741 226
Fragments arranged by size:
741 533 226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1047 1422 1500
Size: 1047 375 78
Fragments arranged by size:
1047 375 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 292 1500
Size: 292 1208
AlwNI CAG_nnn'CTG
Cuts at: 0 27 208 1500
Size: 27 181 1292
Fragments arranged by size:
1292 181 27
ApaI G_GGCC'C
Cuts at: 0 1020 1500
Size: 1020 480
ApoI r'AATT_y
Cuts at: 0 112 1500
Size: 112 1388
BamHI G'GATC_C
Cuts at: 0 70 1500
Size: 70 1430
BanI G'GyrC_C
Cuts at: 0 63 533 1274 1309 1500
Size: 63 470 741 35 191
Fragments arranged by size:
741 470 191 63 35
BanII G_rGCy'C
Cuts at: 0 881 1020 1500
Size: 881 139 480
Fragments arranged by size:
881 480 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 859 1428 1500
Size: 145 714 569 72
Fragments arranged by size:
714 569 145 72
BbvCI CC'TCA_GC
Cuts at: 0 1178 1500
Size: 1178 322
BglI GCCn_nnn'nGGC
Cuts at: 0 1237 1500
Size: 1237 263
BglII A'GATC_T
Cuts at: 0 160 296 1500
Size: 160 136 1204
Fragments arranged by size:
1204 160 136
BlpI GC'TnA_GC
Cuts at: 0 672 1500
Size: 672 828
Bme1580I G_kGCm'C
Cuts at: 0 1020 1500
Size: 1020 480
BmgBI CAC'GTC
Cuts at: 0 587 1053 1500
Size: 587 466 447
Fragments arranged by size:
587 466 447
BmrI ACTGGGnnnn_n'
Cuts at: 0 1038 1091 1275 1500
Size: 1038 53 184 225
Fragments arranged by size:
1038 225 184 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1249 1500
Size: 601 648 251
Fragments arranged by size:
648 601 251
Bpu10I CC'TnA_GC
Cuts at: 0 1178 1500
Size: 1178 322
BsaBI GATnn'nnATC
Cuts at: 0 709 1500
Size: 709 791
BsaWI w'CCGG_w
Cuts at: 0 405 536 988 1127 1500
Size: 405 131 452 139 373
Fragments arranged by size:
452 405 373 139 131
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 589 808 1007 1117 1500
Size: 291 298 219 199 110 383
Fragments arranged by size:
383 298 291 219 199 110
BseYI C'CCAG_C
Cuts at: 0 20 467 1044 1146 1500
Size: 20 447 577 102 354
Fragments arranged by size:
577 447 354 102 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1500
Size: 984 516
BsiHKAI G_wGCw'C
Cuts at: 0 1028 1500
Size: 1028 472
BsmI GAATG_Cn'
Cuts at: 0 204 281 1500
Size: 204 77 1219
Fragments arranged by size:
1219 204 77
BsmBI CGTCTCn'nnnn_
Cuts at: 0 577 1500
Size: 577 923
Bsp1286I G_dGCh'C
Cuts at: 0 881 1020 1028 1500
Size: 881 139 8 472
Fragments arranged by size:
881 472 139 8
BspEI T'CCGG_A
Cuts at: 0 405 1500
Size: 405 1095
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1217 1500
Size: 1217 283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 438 1228 1316 1500
Size: 438 790 88 184
Fragments arranged by size:
790 438 184 88
BstXI CCAn_nnnn'nTGG
Cuts at: 0 607 1500
Size: 607 893
BstYI r'GATC_y
Cuts at: 0 70 160 282 296 822 1130 1500
Size: 70 90 122 14 526 308 370
Fragments arranged by size:
526 370 308 122 90 70 14
BtgI C'CryG_G
Cuts at: 0 479 651 662 745 1500
Size: 479 172 11 83 755
Fragments arranged by size:
755 479 172 83 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 717 927 1500
Size: 717 210 573
Fragments arranged by size:
717 573 210
DrdI GACnn_nn'nnGTC
Cuts at: 0 83 1500
Size: 83 1417
EaeI y'GGCC_r
Cuts at: 0 860 1500
Size: 860 640
EarI CTCTTCn'nnn_
Cuts at: 0 227 761 806 1500
Size: 227 534 45 694
Fragments arranged by size:
694 534 227 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1047 1249 1422 1500
Size: 601 446 202 173 78
Fragments arranged by size:
601 446 202 173 78
EcoO109I rG'GnC_Cy
Cuts at: 0 154 603 1032 1152 1206 1239 1500
Size: 154 449 429 120 54 33 261
Fragments arranged by size:
449 429 261 154 120 54 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 540 1500
Size: 540 960
HaeII r_GCGC'y
Cuts at: 0 27 1500
Size: 27 1473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 374 406 1500
Size: 374 32 1094
Fragments arranged by size:
1094 374 32
HincII GTy'rAC
Cuts at: 0 105 378 1500
Size: 105 273 1122
Fragments arranged by size:
1122 273 105
KpnI G_GTAC'C
Cuts at: 0 537 1278 1500
Size: 537 741 222
Fragments arranged by size:
741 537 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 1500
Size: 372 1128
MslI CAynn'nnrTG
Cuts at: 0 478 526 1500
Size: 478 48 974
Fragments arranged by size:
974 478 48
MspA1I CmG'CkG
Cuts at: 0 183 803 1500
Size: 183 620 697
Fragments arranged by size:
697 620 183
NcoI C'CATG_G
Cuts at: 0 479 651 662 1500
Size: 479 172 11 838
Fragments arranged by size:
838 479 172 11
NspI r_CATG'y
Cuts at: 0 372 1500
Size: 372 1128
PasI CC'CwG_GG
Cuts at: 0 151 1500
Size: 151 1349
PflMI CCAn_nnn'nTGG
Cuts at: 0 27 1500
Size: 27 1473
PpuMI rG'GwC_Cy
Cuts at: 0 1152 1206 1500
Size: 1152 54 294
Fragments arranged by size:
1152 294 54
PshAI GACnn'nnGTC
Cuts at: 0 145 1500
Size: 145 1355
PspOMI G'GGCC_C
Cuts at: 0 1016 1500
Size: 1016 484
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1267 1299 1500
Size: 1267 32 201
Fragments arranged by size:
1267 201 32
PstI C_TGCA'G
Cuts at: 0 37 96 1500
Size: 37 59 1404
Fragments arranged by size:
1404 59 37
PvuII CAG'CTG
Cuts at: 0 183 1500
Size: 183 1317
SalI G'TCGA_C
Cuts at: 0 103 1500
Size: 103 1397
SanDI GG'GwC_CC
Cuts at: 0 1152 1500
Size: 1152 348
SexAI A'CCwGG_T
Cuts at: 0 251 1500
Size: 251 1249
SfcI C'TryA_G
Cuts at: 0 33 92 621 1500
Size: 33 59 529 879
Fragments arranged by size:
879 529 59 33
SspI AAT'ATT
Cuts at: 0 1470 1500
Size: 1470 30
StuI AGG'CCT
Cuts at: 0 1113 1500
Size: 1113 387
StyI C'CwwG_G
Cuts at: 0 479 651 662 1500
Size: 479 172 11 838
Fragments arranged by size:
838 479 172 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1381 1500
Size: 1381 119
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 892 1500
Size: 892 608
Enzymes that do cut:
AccI Acc65I AcuI AlfI AlwNI ApaI ApoI BamHI
BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaBI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BstAPI
BstXI BstYI BtgI BtgZI DrdI EaeI EarI Eco57MI
EcoO109I FalI HaeII Hin4I HincII KpnI MmeI MslI
MspA1I NcoI NspI PasI PflMI PpuMI PshAI PspOMI
PsrI PstI PvuII SalI SanDI SexAI SfcI SspI
StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BmtI BplI
BpuEI BsaI BsaAI BsaHI BsaXI BsiEI BsiWI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PsiI PspXI PvuI RsrII
SacI SacII SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI