(Linear) (Six-base) MAPSORT of: KIBB3590.seq  Check: 5186  from: 1  to: 393



KIBB3590.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     393 
   Size:        127     266
 
AlwNI CAG_nnn'CTG

Cuts at:      0     275     393 
   Size:        275     118
 
BanI G'GyrC_C

Cuts at:      0     343     393 
   Size:        343      50
 
BglII A'GATC_T

Cuts at:      0       5     393 
   Size:          5     388
 
BlpI GC'TnA_GC

Cuts at:      0     358     393 
   Size:        358      35
 
Bpu10I CC'TnA_GC

Cuts at:      0     199     393 
   Size:        199     194
 
BsaHI Gr'CG_yC

Cuts at:      0     239     393 
   Size:        239     154
 
BseYI C'CCAG_C

Cuts at:      0     339     393 
   Size:        339      54
 
BsiEI CG_ry'CG

Cuts at:      0     308     393 
   Size:        308      85
 
BsrFI r'CCGG_y

Cuts at:      0     163     369     393 
   Size:        163     206      24

  Fragments arranged by size: 

                 206     163      24
 
BssSI C'ACGA_G

Cuts at:      0     178     393 
   Size:        178     215
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     272     393 
   Size:        272     121
 
BstYI r'GATC_y

Cuts at:      0       5     393 
   Size:          5     388
 
BtgI C'CryG_G

Cuts at:      0     260     393 
   Size:        260     133
 
EaeI y'GGCC_r

Cuts at:      0     189     305     393 
   Size:        189     116      88

  Fragments arranged by size: 

                 189     116      88
 
EagI C'GGCC_G

Cuts at:      0     305     393 
   Size:        305      88
 
EarI CTCTTCn'nnn_

Cuts at:      0      82     393 
   Size:         82     311
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     393 
   Size:        127     266
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      27     393 
   Size:         27     366
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     393 
   Size:        149     244
 
MscI TGG'CCA

Cuts at:      0     191     393 
   Size:        191     202
 
MspA1I CmG'CkG

Cuts at:      0     272     393 
   Size:        272     121
 
NaeI GCC'GGC

Cuts at:      0     165     371     393 
   Size:        165     206      22

  Fragments arranged by size: 

                 206     165      22
 
NcoI C'CATG_G

Cuts at:      0     260     393 
   Size:        260     133
 
NgoMIV G'CCGG_C

Cuts at:      0     163     369     393 
   Size:        163     206      24

  Fragments arranged by size: 

                 206     163      24
 
PvuII CAG'CTG

Cuts at:      0     272     393 
   Size:        272     121
 
SapI GCTCTTCn'nnn_

Cuts at:      0      82     393 
   Size:         82     311
 
StyI C'CwwG_G

Cuts at:      0     260     393 
   Size:        260     133
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      28     331     393 
   Size:         28     303      62

  Fragments arranged by size: 

                 303      62      28
 
TatI w'GTAC_w

Cuts at:      0      98     393 
   Size:         98     295
 
Tth111I GACn'n_nGTC

Cuts at:      0     150     393 
   Size:        150     243
 
XmnI GAAnn'nnTTC

Cuts at:      0       7     393 
   Size:          7     386
 

 Enzymes that do cut:

     AcuI    AlwNI     BanI    BglII     BlpI   Bpu10I    BsaHI    BseYI
    BsiEI    BsrFI    BssSI   BstAPI    BstYI     BtgI     EaeI     EagI
     EarI  Eco57MI     FalI     MmeI     MscI   MspA1I     NaeI     NcoI
   NgoMIV    PvuII     SapI     StyI    TaqII     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MslI     NarI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI
     XcmI     XhoI     XmaI     ZraI