Restriction map for
pF1KB3513
(Linear) (Six-base) MAPSORT of: KIBB3513.seq Check: 2708 from: 1 to: 3069
KIBB3513.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2758 3069
Size: 2758 311
AccI GT'mk_AC
Cuts at: 0 776 3069
Size: 776 2293
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 1226 1718 3069
Size: 124 1102 492 1351
Fragments arranged by size:
1351 1102 492 124
AflII C'TTAA_G
Cuts at: 0 1327 3069
Size: 1327 1742
AflIII A'CryG_T
Cuts at: 0 1169 3069
Size: 1169 1900
AleI CACnn'nnGTG
Cuts at: 0 889 2000 3069
Size: 889 1111 1069
Fragments arranged by size:
1111 1069 889
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 645 3069
Size: 645 2424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 857 889 3069
Size: 857 32 2180
Fragments arranged by size:
2180 857 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1587 2586 3069
Size: 1587 999 483
Fragments arranged by size:
1587 999 483
ApoI r'AATT_y
Cuts at: 0 593 1728 2823 3069
Size: 593 1135 1095 246
Fragments arranged by size:
1135 1095 593 246
AvaI C'yCGr_G
Cuts at: 0 2353 3069
Size: 2353 716
AvrII C'CTAG_G
Cuts at: 0 1656 3069
Size: 1656 1413
BamHI G'GATC_C
Cuts at: 0 1538 3069
Size: 1538 1531
BanI G'GyrC_C
Cuts at: 0 754 1525 3069
Size: 754 771 1544
Fragments arranged by size:
1544 771 754
BanII G_rGCy'C
Cuts at: 0 1502 1763 3069
Size: 1502 261 1306
Fragments arranged by size:
1502 1306 261
BbeI G_GCGC'C
Cuts at: 0 1529 3069
Size: 1529 1540
BbsI GAAGACnn'nnnn_
Cuts at: 0 1931 2702 2823 3069
Size: 1931 771 121 246
Fragments arranged by size:
1931 771 246 121
BbvCI CC'TCA_GC
Cuts at: 0 497 2084 3069
Size: 497 1587 985
Fragments arranged by size:
1587 985 497
BciVI GTATCCnnnnn_n'
Cuts at: 0 2951 3069
Size: 2951 118
BclI T'GATC_A
Cuts at: 0 126 2546 3069
Size: 126 2420 523
Fragments arranged by size:
2420 523 126
BglI GCCn_nnn'nGGC
Cuts at: 0 3041 3069
Size: 3041 28
BglII A'GATC_T
Cuts at: 0 2855 3069
Size: 2855 214
Bme1580I G_kGCm'C
Cuts at: 0 2440 3069
Size: 2440 629
BmgBI CAC'GTC
Cuts at: 0 19 1109 3069
Size: 19 1090 1960
Fragments arranged by size:
1960 1090 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 791 1945 2413 2676 3069
Size: 791 1154 468 263 393
Fragments arranged by size:
1154 791 468 393 263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 730 2150 2300 3069
Size: 668 62 1420 150 769
Fragments arranged by size:
1420 769 668 150 62
Bpu10I CC'TnA_GC
Cuts at: 0 497 2084 3069
Size: 497 1587 985
Fragments arranged by size:
1587 985 497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 958 973 3069
Size: 195 763 15 2096
Fragments arranged by size:
2096 763 195 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 581 686 1087 1847 1909 3069
Size: 581 105 401 760 62 1160
Fragments arranged by size:
1160 760 581 401 105 62
BsaAI yAC'GTr
Cuts at: 0 763 3069
Size: 763 2306
BsaBI GATnn'nnATC
Cuts at: 0 189 3069
Size: 189 2880
BsaHI Gr'CG_yC
Cuts at: 0 1526 1607 3033 3069
Size: 1526 81 1426 36
Fragments arranged by size:
1526 1426 81 36
BsaWI w'CCGG_w
Cuts at: 0 1151 3069
Size: 1151 1918
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 231 261 666 696 1637 1667 2005 2035
Size: 231 30 405 30 941 30 338 30
Cuts at: 2035 3069
Size: 1034
Fragments arranged by size:
1034 941 405 338 231 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 599 636 1555 1609 3069
Size: 599 37 919 54 1460
Fragments arranged by size:
1460 919 599 54 37
BseYI C'CCAG_C
Cuts at: 0 1632 3044 3069
Size: 1632 1412 25
Fragments arranged by size:
1632 1412 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 2806 3069
Size: 221 2585 263
Fragments arranged by size:
2585 263 221
BsiEI CG_ry'CG
Cuts at: 0 1793 3069
Size: 1793 1276
BsmBI CGTCTCn'nnnn_
Cuts at: 0 706 3069
Size: 706 2363
Bsp1286I G_dGCh'C
Cuts at: 0 1502 1763 2440 3069
Size: 1502 261 677 629
Fragments arranged by size:
1502 677 629 261
BspHI T'CATG_A
Cuts at: 0 2492 3069
Size: 2492 577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1264 2758 3069
Size: 1264 1494 311
Fragments arranged by size:
1494 1264 311
BsrBI CCG'CTC
Cuts at: 0 2541 3069
Size: 2541 528
BsrDI GCAATG_nn'
Cuts at: 0 722 848 1917 1928 2324 3069
Size: 722 126 1069 11 396 745
Fragments arranged by size:
1069 745 722 396 126 11
BssSI C'ACGA_G
Cuts at: 0 197 3069
Size: 197 2872
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1348 3069
Size: 1348 1721
BstBI TT'CG_AA
Cuts at: 0 516 3069
Size: 516 2553
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1050 1890 2463 3069
Size: 1050 840 573 606
Fragments arranged by size:
1050 840 606 573
BstYI r'GATC_y
Cuts at: 0 209 564 1538 2855 3069
Size: 209 355 974 1317 214
Fragments arranged by size:
1317 974 355 214 209
BtgI C'CryG_G
Cuts at: 0 1569 1783 1805 1919 3069
Size: 1569 214 22 114 1150
Fragments arranged by size:
1569 1150 214 114 22
BtsI GCAGTG_nn'
Cuts at: 0 1298 2007 2719 2794 3069
Size: 1298 709 712 75 275
Fragments arranged by size:
1298 712 709 275 75
ClaI AT'CG_AT
Cuts at: 0 1739 3069
Size: 1739 1330
DrdI GACnn_nn'nnGTC
Cuts at: 0 1101 3069
Size: 1101 1968
EaeI y'GGCC_r
Cuts at: 0 1010 1790 3069
Size: 1010 780 1279
Fragments arranged by size:
1279 1010 780
EagI C'GGCC_G
Cuts at: 0 1790 3069
Size: 1790 1279
EarI CTCTTCn'nnn_
Cuts at: 0 359 2491 2873 2990 3069
Size: 359 2132 382 117 79
Fragments arranged by size:
2132 382 359 117 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 668 730 1226 1718 2150 2300 3069
Size: 124 544 62 496 492 432 150 769
Fragments arranged by size:
769 544 496 492 432 150 124 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1591 3069
Size: 1591 1478
EcoO109I rG'GnC_Cy
Cuts at: 0 1638 2075 2583 3069
Size: 1638 437 508 486
Fragments arranged by size:
1638 508 486 437
EcoRI G'AATT_C
Cuts at: 0 593 2823 3069
Size: 593 2230 246
Fragments arranged by size:
2230 593 246
HaeII r_GCGC'y
Cuts at: 0 1529 3069
Size: 1529 1540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 631 663 666 698 1197 1229 1637 1669
Size: 631 32 3 32 499 32 408 32
Cuts at: 1669 1764 1796 2005 2037 2067 2099 3069
Size: 95 32 209 32 30 32 970
Fragments arranged by size:
970 631 499 408 209 95 32 32
32 32 32 32 32 30 3
KasI G'GCGC_C
Cuts at: 0 1525 3069
Size: 1525 1544
MfeI C'AATT_G
Cuts at: 0 741 2270 3069
Size: 741 1529 799
Fragments arranged by size:
1529 799 741
MscI TGG'CCA
Cuts at: 0 1012 3069
Size: 1012 2057
MslI CAynn'nnrTG
Cuts at: 0 796 889 1768 2000 2101 2443 3069
Size: 796 93 879 232 101 342 626
Fragments arranged by size:
879 796 626 342 232 101 93
MspA1I CmG'CkG
Cuts at: 0 40 205 2031 2678 3069
Size: 40 165 1826 647 391
Fragments arranged by size:
1826 647 391 165 40
NarI GG'CG_CC
Cuts at: 0 1526 3069
Size: 1526 1543
NspI r_CATG'y
Cuts at: 0 1173 3069
Size: 1173 1896
PasI CC'CwG_GG
Cuts at: 0 1970 2586 3069
Size: 1970 616 483
Fragments arranged by size:
1970 616 483
PciI A'CATG_T
Cuts at: 0 1169 3069
Size: 1169 1900
PflMI CCAn_nnn'nTGG
Cuts at: 0 2586 3069
Size: 2586 483
PmlI CAC'GTG
Cuts at: 0 763 3069
Size: 763 2306
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1128 1160 3069
Size: 1128 32 1909
Fragments arranged by size:
1909 1128 32
PpuMI rG'GwC_Cy
Cuts at: 0 2075 3069
Size: 2075 994
PstI C_TGCA'G
Cuts at: 0 39 858 3069
Size: 39 819 2211
Fragments arranged by size:
2211 819 39
PvuII CAG'CTG
Cuts at: 0 40 205 2031 3069
Size: 40 165 1826 1038
Fragments arranged by size:
1826 1038 165 40
SexAI A'CCwGG_T
Cuts at: 0 2956 3069
Size: 2956 113
SfcI C'TryA_G
Cuts at: 0 35 359 854 3069
Size: 35 324 495 2215
Fragments arranged by size:
2215 495 324 35
SfoI GGC'GCC
Cuts at: 0 1527 3069
Size: 1527 1542
SmlI C'TyrA_G
Cuts at: 0 174 937 952 1327 3069
Size: 174 763 15 375 1742
Fragments arranged by size:
1742 763 375 174 15
StuI AGG'CCT
Cuts at: 0 1984 3069
Size: 1984 1085
StyI C'CwwG_G
Cuts at: 0 1097 1656 1979 3069
Size: 1097 559 323 1090
Fragments arranged by size:
1097 1090 559 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 605 895 2817 2911 3036 3069
Size: 385 220 290 1922 94 125 33
Fragments arranged by size:
1922 385 290 220 125 94 33
TatI w'GTAC_w
Cuts at: 0 941 1992 3069
Size: 941 1051 1077
Fragments arranged by size:
1077 1051 941
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 231 263 3069
Size: 231 32 2806
Fragments arranged by size:
2806 231 32
Tth111I GACn'n_nGTC
Cuts at: 0 678 2800 3007 3069
Size: 678 2122 207 62
Fragments arranged by size:
2122 678 207 62
XmnI GAAnn'nnTTC
Cuts at: 0 1691 2629 3069
Size: 1691 938 440
Fragments arranged by size:
1691 938 440
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI AlfI AloI
AlwNI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BssSI BstAPI BstBI BstXI
BstYI BtgI BtsI ClaI DrdI EaeI EagI EarI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MfeI
MscI MslI MspA1I NarI NspI PasI PciI PflMI
PmlI PpiI PpuMI PstI PvuII SexAI SfcI SfoI
SmlI StuI StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI ApaI ApaLI
AscI AseI AsiSI BaeI BcgI BfrBI BlpI BmtI
BplI BsiHKAI BsiWI BsmI BspEI BsrFI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII EciI
EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MluI MmeI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XcmI XhoI XmaI ZraI