(Linear) (Six-base) MAPSORT of: KIBB3512.seq  Check: 256  from: 1  to: 1242



KIBB3512.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AflII C'TTAA_G

Cuts at:      0     415    1242 
   Size:        415     827
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     746     778    1242 
   Size:        746      32     464

  Fragments arranged by size: 

                 746     464      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     196     357    1242 
   Size:        196     161     885

  Fragments arranged by size: 

                 885     196     161
 
ApoI r'AATT_y

Cuts at:      0     176    1242 
   Size:        176    1066
 
AseI AT'TA_AT

Cuts at:      0     953    1242 
   Size:        953     289
 
BanII G_rGCy'C

Cuts at:      0      44    1242 
   Size:         44    1198
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      68     651    1217    1242 
   Size:         68     583     566      25

  Fragments arranged by size: 

                 583     566      68      25
 
BclI T'GATC_A

Cuts at:      0     708     743    1242 
   Size:        708      35     499

  Fragments arranged by size: 

                 708     499      35
 
BglI GCCn_nnn'nGGC

Cuts at:      0     967     979    1242 
   Size:        967      12     263

  Fragments arranged by size: 

                 967     263      12
 
BglII A'GATC_T

Cuts at:      0     411    1242 
   Size:        411     831
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      93    1242 
   Size:         93    1149
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     648    1242 
   Size:        648     594
 
Bpu10I CC'TnA_GC

Cuts at:      0     785    1242 
   Size:        785     457
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1191    1242 
   Size:       1191      51
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     334    1242 
   Size:        334     908
 
BsaBI GATnn'nnATC

Cuts at:      0    1161    1242 
   Size:       1161      81
 
BseYI C'CCAG_C

Cuts at:      0     200    1232    1242 
   Size:        200    1032      10

  Fragments arranged by size: 

                1032     200      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418    1160    1242 
   Size:        418     742      82

  Fragments arranged by size: 

                 742     418      82
 
Bsp1286I G_dGCh'C

Cuts at:      0      44    1242 
   Size:         44    1198
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     390    1242 
   Size:        390     852
 
BsrGI T'GTAC_A

Cuts at:      0     592    1242 
   Size:        592     650
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1168    1242 
   Size:       1168      74
 
BstEII G'GTnAC_C

Cuts at:      0      18    1242 
   Size:         18    1224
 
BstYI r'GATC_y

Cuts at:      0     411    1242 
   Size:        411     831
 
BtgI C'CryG_G

Cuts at:      0      86     334    1242 
   Size:         86     248     908

  Fragments arranged by size: 

                 908     248      86
 
BtsI GCAGTG_nn'

Cuts at:      0     284    1188    1242 
   Size:        284     904      54

  Fragments arranged by size: 

                 904     284      54
 
EcoO109I rG'GnC_Cy

Cuts at:      0     354     680    1242 
   Size:        354     326     562

  Fragments arranged by size: 

                 562     354     326
 
EcoRI G'AATT_C

Cuts at:      0     176    1242 
   Size:        176    1066
 
FspI TGC'GCA

Cuts at:      0     719    1242 
   Size:        719     523
 
HaeII r_GCGC'y

Cuts at:      0     591    1242 
   Size:        591     651
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288    1207    1239    1242 
   Size:        256      32     919      32       3

  Fragments arranged by size: 

                 919     256      32      32       3
 
HincII GTy'rAC

Cuts at:      0     225    1242 
   Size:        225    1017
 
MfeI C'AATT_G

Cuts at:      0    1145    1242 
   Size:       1145      97
 
NcoI C'CATG_G

Cuts at:      0     334    1242 
   Size:        334     908
 
NdeI CA'TA_TG

Cuts at:      0     497    1242 
   Size:        497     745
 
NspI r_CATG'y

Cuts at:      0     187    1017    1077    1242 
   Size:        187     830      60     165

  Fragments arranged by size: 

                 830     187     165      60
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     196    1242 
   Size:        196    1046
 
PpuMI rG'GwC_Cy

Cuts at:      0     354    1242 
   Size:        354     888
 
SfcI C'TryA_G

Cuts at:      0     126     988    1242 
   Size:        126     862     254

  Fragments arranged by size: 

                 862     254     126
 
SmlI C'TyrA_G

Cuts at:      0     415    1206    1242 
   Size:        415     791      36

  Fragments arranged by size: 

                 791     415      36
 
SphI G_CATG'C

Cuts at:      0     187    1242 
   Size:        187    1055
 
StuI AGG'CCT

Cuts at:      0    1096    1242 
   Size:       1096     146
 
StyI C'CwwG_G

Cuts at:      0     334    1065    1242 
   Size:        334     731     177

  Fragments arranged by size: 

                 731     334     177
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     910    1242 
   Size:        910     332
 
TatI w'GTAC_w

Cuts at:      0     592    1242 
   Size:        592     650
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     197     229     843     875    1242 
   Size:        197      32     614      32     367

  Fragments arranged by size: 

                 614     367     197      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     197    1242 
   Size:        197    1045
 
XmnI GAAnn'nnTTC

Cuts at:      0      11     176    1242 
   Size:         11     165    1066

  Fragments arranged by size: 

                1066     165      11
 

 Enzymes that do cut:

    AflII     AloI    AlwNI     ApoI     AseI    BanII     BbsI     BclI
     BglI    BglII     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BseYI     BsgI Bsp1286I    BspMI    BsrGI   BstAPI   BstEII    BstYI
     BtgI     BtsI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
     MfeI     NcoI     NdeI     NspI    PflMI    PpuMI     SfcI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BlpI Bme1580I    BmgBI     BmtI     BpmI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRV     FalI     FseI    FspAI  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I
     XbaI     XhoI     XmaI     ZraI