(Linear) (Six-base) MAPSORT of: KIBB3509.seq  Check: 3959  from: 1  to: 1098



KIBB3509.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227    1098 
   Size:        227     871
 
AhdI GACnn_n'nnGTC

Cuts at:      0     379    1098 
   Size:        379     719
 
AleI CACnn'nnGTG

Cuts at:      0     589    1098 
   Size:        589     509
 
ApaI G_GGCC'C

Cuts at:      0      11    1098 
   Size:         11    1087
 
ApaLI G'TGCA_C

Cuts at:      0     592    1098 
   Size:        592     506
 
ApoI r'AATT_y

Cuts at:      0     134     330     676     756    1098 
   Size:        134     196     346      80     342

  Fragments arranged by size: 

                 346     342     196     134      80
 
BanII G_rGCy'C

Cuts at:      0      11    1098 
   Size:         11    1087
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     304    1098 
   Size:        304     794
 
Bme1580I G_kGCm'C

Cuts at:      0      11     596    1098 
   Size:         11     585     502

  Fragments arranged by size: 

                 585     502      11
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     863    1098 
   Size:        242     621     235

  Fragments arranged by size: 

                 621     242     235
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      33     811    1098 
   Size:         33     778     287

  Fragments arranged by size: 

                 778     287      33
 
BsaWI w'CCGG_w

Cuts at:      0     572    1098 
   Size:        572     526
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205    1098 
   Size:        205     893
 
BseYI C'CCAG_C

Cuts at:      0       4      39    1098 
   Size:          4      35    1059

  Fragments arranged by size: 

                1059      35       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     809    1098 
   Size:        514     295     289

  Fragments arranged by size: 

                 514     295     289
 
BsiHKAI G_wGCw'C

Cuts at:      0     596    1098 
   Size:        596     502
 
BsmI GAATG_Cn'

Cuts at:      0      46    1098 
   Size:         46    1052
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     596    1098 
   Size:         11     585     502

  Fragments arranged by size: 

                 585     502      11
 
BsrBI CCG'CTC

Cuts at:      0     195    1098 
   Size:        195     903
 
BsrDI GCAATG_nn'

Cuts at:      0     533     884    1047    1098 
   Size:        533     351     163      51

  Fragments arranged by size: 

                 533     351     163      51
 
BsrFI r'CCGG_y

Cuts at:      0     313    1098 
   Size:        313     785
 
BstEII G'GTnAC_C

Cuts at:      0      66     316    1098 
   Size:         66     250     782

  Fragments arranged by size: 

                 782     250      66
 
BstYI r'GATC_y

Cuts at:      0      17     723    1098 
   Size:         17     706     375

  Fragments arranged by size: 

                 706     375      17
 
BtgI C'CryG_G

Cuts at:      0     260    1098 
   Size:        260     838
 
BtsI GCAGTG_nn'

Cuts at:      0     203    1098 
   Size:        203     895
 
DraIII CAC_nnn'GTG

Cuts at:      0     823    1098 
   Size:        823     275
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     242     863    1098 
   Size:        227      15     621     235

  Fragments arranged by size: 

                 621     235     227      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     217     289    1098 
   Size:        217      72     809

  Fragments arranged by size: 

                 809     217      72
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     297    1098 
   Size:        297     801
 
FspI TGC'GCA

Cuts at:      0     343    1098 
   Size:        343     755
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     365     397     789     821    1098 
   Size:        365      32     392      32     277

  Fragments arranged by size: 

                 392     365     277      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     396    1098 
   Size:        396     702
 
MslI CAynn'nnrTG

Cuts at:      0     589     613    1098 
   Size:        589      24     485

  Fragments arranged by size: 

                 589     485      24
 
MspA1I CmG'CkG

Cuts at:      0     142    1098 
   Size:        142     956
 
NcoI C'CATG_G

Cuts at:      0     260    1098 
   Size:        260     838
 
NspI r_CATG'y

Cuts at:      0     475    1098 
   Size:        475     623
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     118    1098 
   Size:        118     980
 
PfoI T'CCnGG_A

Cuts at:      0     111    1098 
   Size:        111     987
 
PpuMI rG'GwC_Cy

Cuts at:      0     217    1098 
   Size:        217     881
 
PspOMI G'GGCC_C

Cuts at:      0       7    1098 
   Size:          7    1091
 
PstI C_TGCA'G

Cuts at:      0     147    1098 
   Size:        147     951
 
PvuII CAG'CTG

Cuts at:      0     142    1098 
   Size:        142     956
 
SfcI C'TryA_G

Cuts at:      0     143    1098 
   Size:        143     955
 
SspI AAT'ATT

Cuts at:      0     423    1098 
   Size:        423     675
 
StyI C'CwwG_G

Cuts at:      0     260    1098 
   Size:        260     838
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     335    1098 
   Size:        335     763
 

 Enzymes that do cut:

     AcuI     AhdI     AleI     ApaI    ApaLI     ApoI    BanII     BbsI
 Bme1580I     BpmI     BsaI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BsrBI    BsrDI    BsrFI   BstEII    BstYI     BtgI
     BtsI   DraIII  Eco57MI EcoO109I     FalI     FspI    Hin4I     MmeI
     MslI   MspA1I     NcoI     NspI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SfcI     SspI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AlfI     AloI    AlwNI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI