Restriction map for
pF1KB3506
(Linear) (Six-base) MAPSORT of: KIBB3506.seq Check: 3206 from: 1 to: 807
KIBB3506.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 807
Size: 600 207
AlwNI CAG_nnn'CTG
Cuts at: 0 414 807
Size: 414 393
AvaI C'yCGr_G
Cuts at: 0 402 807
Size: 402 405
BanI G'GyrC_C
Cuts at: 0 214 479 807
Size: 214 265 328
Fragments arranged by size:
328 265 214
BanII G_rGCy'C
Cuts at: 0 663 807
Size: 663 144
BbeI G_GCGC'C
Cuts at: 0 218 483 807
Size: 218 265 324
Fragments arranged by size:
324 265 218
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 807
Size: 66 741
BclI T'GATC_A
Cuts at: 0 602 807
Size: 602 205
BglI GCCn_nnn'nGGC
Cuts at: 0 187 807
Size: 187 620
BlpI GC'TnA_GC
Cuts at: 0 172 253 498 807
Size: 172 81 245 309
Fragments arranged by size:
309 245 172 81
Bme1580I G_kGCm'C
Cuts at: 0 282 807
Size: 282 525
BmrI ACTGGGnnnn_n'
Cuts at: 0 250 294 368 807
Size: 250 44 74 439
Fragments arranged by size:
439 250 74 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 424 768 807
Size: 51 373 344 39
Fragments arranged by size:
373 344 51 39
BsaBI GATnn'nnATC
Cuts at: 0 268 807
Size: 268 539
BsaHI Gr'CG_yC
Cuts at: 0 215 480 807
Size: 215 265 327
Fragments arranged by size:
327 265 215
BsaWI w'CCGG_w
Cuts at: 0 473 807
Size: 473 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 468 498 766 796 807
Size: 468 30 268 30 11
Fragments arranged by size:
468 268 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 339 766 807
Size: 339 427 41
Fragments arranged by size:
427 339 41
BsiEI CG_ry'CG
Cuts at: 0 474 807
Size: 474 333
Bsp1286I G_dGCh'C
Cuts at: 0 282 663 807
Size: 282 381 144
Fragments arranged by size:
381 282 144
BsrDI GCAATG_nn'
Cuts at: 0 384 807
Size: 384 423
BsrGI T'GTAC_A
Cuts at: 0 287 807
Size: 287 520
BtgI C'CryG_G
Cuts at: 0 596 807
Size: 596 211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 162 279 807
Size: 162 117 528
Fragments arranged by size:
528 162 117
BtsI GCAGTG_nn'
Cuts at: 0 44 711 807
Size: 44 667 96
Fragments arranged by size:
667 96 44
EaeI y'GGCC_r
Cuts at: 0 2 44 56 372 741 807
Size: 2 42 12 316 369 66
Fragments arranged by size:
369 316 66 42 12 2
EarI CTCTTCn'nnn_
Cuts at: 0 31 807
Size: 31 776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 424 600 768 807
Size: 51 373 176 168 39
Fragments arranged by size:
373 176 168 51 39
EcoO109I rG'GnC_Cy
Cuts at: 0 411 434 807
Size: 411 23 373
Fragments arranged by size:
411 373 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 807
Size: 42 765
HaeII r_GCGC'y
Cuts at: 0 218 483 807
Size: 218 265 324
Fragments arranged by size:
324 265 218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 766 798 807
Size: 766 32 9
Fragments arranged by size:
766 32 9
KasI G'GCGC_C
Cuts at: 0 214 479 807
Size: 214 265 328
Fragments arranged by size:
328 265 214
MscI TGG'CCA
Cuts at: 0 4 58 807
Size: 4 54 749
Fragments arranged by size:
749 54 4
MspA1I CmG'CkG
Cuts at: 0 212 257 444 598 740 807
Size: 212 45 187 154 142 67
Fragments arranged by size:
212 187 154 142 67 45
NarI GG'CG_CC
Cuts at: 0 215 480 807
Size: 215 265 327
Fragments arranged by size:
327 265 215
PasI CC'CwG_GG
Cuts at: 0 96 807
Size: 96 711
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 414 807
Size: 306 108 393
Fragments arranged by size:
393 306 108
PfoI T'CCnGG_A
Cuts at: 0 137 807
Size: 137 670
PpuMI rG'GwC_Cy
Cuts at: 0 434 807
Size: 434 373
PshAI GACnn'nnGTC
Cuts at: 0 133 807
Size: 133 674
PvuII CAG'CTG
Cuts at: 0 257 444 807
Size: 257 187 363
Fragments arranged by size:
363 257 187
SacII CC_GC'GG
Cuts at: 0 599 807
Size: 599 208
SanDI GG'GwC_CC
Cuts at: 0 434 807
Size: 434 373
SapI GCTCTTCn'nnn_
Cuts at: 0 31 807
Size: 31 776
SfcI C'TryA_G
Cuts at: 0 524 807
Size: 524 283
SfoI GGC'GCC
Cuts at: 0 216 481 807
Size: 216 265 326
Fragments arranged by size:
326 265 216
StyI C'CwwG_G
Cuts at: 0 489 531 807
Size: 489 42 276
Fragments arranged by size:
489 276 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 572 627 807
Size: 572 55 180
Fragments arranged by size:
572 180 55
TatI w'GTAC_w
Cuts at: 0 287 669 807
Size: 287 382 138
Fragments arranged by size:
382 287 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 468 500 807
Size: 468 32 307
Fragments arranged by size:
468 307 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 744 807
Size: 744 63
XmnI GAAnn'nnTTC
Cuts at: 0 25 807
Size: 25 782
Enzymes that do cut:
AcuI AlwNI AvaI BanI BanII BbeI BbsI BclI
BglI BlpI Bme1580I BmrI BpmI BsaBI BsaHI BsaWI
BsaXI BseRI BsiEI Bsp1286I BsrDI BsrGI BtgI BtgZI
BtsI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I
KasI MscI MspA1I NarI PasI PflMI PfoI PpuMI
PshAI PvuII SacII SanDI SapI SfcI SfoI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI