(Linear) (Six-base) MAPSORT of: KIBB3502.seq  Check: 5429  from: 1  to: 729



KIBB3502.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     729 
   Size:        281     448
 
AlwNI CAG_nnn'CTG

Cuts at:      0      21     729 
   Size:         21     708
 
ApoI r'AATT_y

Cuts at:      0      49     729 
   Size:         49     680
 
AseI AT'TA_AT

Cuts at:      0     200     729 
   Size:        200     529
 
AvaI C'yCGr_G

Cuts at:      0      28     729 
   Size:         28     701
 
BanII G_rGCy'C

Cuts at:      0      47     729 
   Size:         47     682
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     139     341     604     729 
   Size:        139     202     263     125

  Fragments arranged by size: 

                 263     202     139     125
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     650     729 
   Size:        650      79
 
Bpu10I CC'TnA_GC

Cuts at:      0      22     658     729 
   Size:         22     636      71

  Fragments arranged by size: 

                 636      71      22
 
BsaAI yAC'GTr

Cuts at:      0     148     729 
   Size:        148     581
 
BsaBI GATnn'nnATC

Cuts at:      0     233     729 
   Size:        233     496
 
BsaWI w'CCGG_w

Cuts at:      0     330     729 
   Size:        330     399
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     568     729 
   Size:        568     161
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     729 
   Size:        625     104
 
BsiHKAI G_wGCw'C

Cuts at:      0      47     729 
   Size:         47     682
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     729 
   Size:         47     682
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       6     729 
   Size:          6     723
 
BssSI C'ACGA_G

Cuts at:      0      40     729 
   Size:         40     689
 
ClaI AT'CG_AT

Cuts at:      0     182     729 
   Size:        182     547
 
EcoICRI GAG'CTC

Cuts at:      0      45     729 
   Size:         45     684
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     729 
   Size:        281     448
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18      58     191     729 
   Size:         18      40     133     538

  Fragments arranged by size: 

                 538     133      40      18
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     673     729 
   Size:        673      56
 
HindIII A'AGCT_T

Cuts at:      0      91     729 
   Size:         91     638
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     729 
   Size:        310     419
 
MspA1I CmG'CkG

Cuts at:      0     173     729 
   Size:        173     556
 
PpuMI rG'GwC_Cy

Cuts at:      0      18      58     729 
   Size:         18      40     671

  Fragments arranged by size: 

                 671      40      18
 
PspXI vC'TCGA_Gb

Cuts at:      0      28     729 
   Size:         28     701
 
SacI G_AGCT'C

Cuts at:      0      47     729 
   Size:         47     682
 
SfcI C'TryA_G

Cuts at:      0     137     729 
   Size:        137     592
 
SmlI C'TyrA_G

Cuts at:      0      28     729 
   Size:         28     701
 
StuI AGG'CCT

Cuts at:      0     656     729 
   Size:        656      73
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     125     729 
   Size:        125     604
 
XhoI C'TCGA_G

Cuts at:      0      28     729 
   Size:         28     701
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AseI     AvaI    BanII     BbsI     BmrI
   Bpu10I    BsaAI    BsaBI    BsaWI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspMI    BssSI     ClaI  EcoICRI  Eco57MI EcoO109I     FalI  HindIII
     MmeI   MspA1I    PpuMI    PspXI     SacI     SfcI     SmlI     StuI
    TaqII     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaXI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI