Restriction map for
pF1KB3492
(Linear) (Six-base) MAPSORT of: KIBB3492.seq Check: 8211 from: 1 to: 1191
KIBB3492.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 435 1191
Size: 435 756
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1191
Size: 737 454
AflIII A'CryG_T
Cuts at: 0 174 1191
Size: 174 1017
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 276 1161 1191
Size: 276 885 30
Fragments arranged by size:
885 276 30
AlwNI CAG_nnn'CTG
Cuts at: 0 19 96 805 1191
Size: 19 77 709 386
Fragments arranged by size:
709 386 77 19
ApoI r'AATT_y
Cuts at: 0 233 1082 1191
Size: 233 849 109
Fragments arranged by size:
849 233 109
BanI G'GyrC_C
Cuts at: 0 67 1191
Size: 67 1124
BanII G_rGCy'C
Cuts at: 0 103 1191
Size: 103 1088
BfrBI ATG'CAT
Cuts at: 0 460 1191
Size: 460 731
BglI GCCn_nnn'nGGC
Cuts at: 0 139 1191
Size: 139 1052
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 389 1191
Size: 389 802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 1129 1191
Size: 315 814 62
Fragments arranged by size:
814 315 62
Bpu10I CC'TnA_GC
Cuts at: 0 72 1191
Size: 72 1119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 1191
Size: 411 780
BsaWI w'CCGG_w
Cuts at: 0 144 1191
Size: 144 1047
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 126 156 1191
Size: 85 30 11 30 1035
Fragments arranged by size:
1035 85 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 365 1191
Size: 365 826
BseYI C'CCAG_C
Cuts at: 0 741 1191
Size: 741 450
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1191
Size: 248 943
BsmI GAATG_Cn'
Cuts at: 0 9 576 1191
Size: 9 567 615
Fragments arranged by size:
615 567 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 36 1191
Size: 36 1155
Bsp1286I G_dGCh'C
Cuts at: 0 103 1191
Size: 103 1088
BspEI T'CCGG_A
Cuts at: 0 144 1191
Size: 144 1047
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 913 1191
Size: 913 278
BsrDI GCAATG_nn'
Cuts at: 0 1178 1191
Size: 1178 13
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 1063 1191
Size: 339 724 128
Fragments arranged by size:
724 339 128
BstYI r'GATC_y
Cuts at: 0 15 1191
Size: 15 1176
BstZ17I GTA'TAC
Cuts at: 0 436 1191
Size: 436 755
BtgI C'CryG_G
Cuts at: 0 669 1191
Size: 669 522
EaeI y'GGCC_r
Cuts at: 0 585 1191
Size: 585 606
EarI CTCTTCn'nnn_
Cuts at: 0 121 624 1191
Size: 121 503 567
Fragments arranged by size:
567 503 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 737 1129 1191
Size: 315 422 392 62
Fragments arranged by size:
422 392 315 62
EcoO109I rG'GnC_Cy
Cuts at: 0 93 1191
Size: 93 1098
EcoRI G'AATT_C
Cuts at: 0 233 1082 1191
Size: 233 849 109
Fragments arranged by size:
849 233 109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 1191
Size: 726 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1191
Size: 85 32 1074
Fragments arranged by size:
1074 85 32
HincII GTy'rAC
Cuts at: 0 623 945 1191
Size: 623 322 246
Fragments arranged by size:
623 322 246
MscI TGG'CCA
Cuts at: 0 587 1191
Size: 587 604
MspA1I CmG'CkG
Cuts at: 0 490 778 976 1191
Size: 490 288 198 215
Fragments arranged by size:
490 288 215 198
NcoI C'CATG_G
Cuts at: 0 669 1191
Size: 669 522
NdeI CA'TA_TG
Cuts at: 0 476 1191
Size: 476 715
NsiI A_TGCA'T
Cuts at: 0 462 1191
Size: 462 729
NspI r_CATG'y
Cuts at: 0 178 1191
Size: 178 1013
PciI A'CATG_T
Cuts at: 0 174 1191
Size: 174 1017
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 509 1191
Size: 96 413 682
Fragments arranged by size:
682 413 96
PfoI T'CCnGG_A
Cuts at: 0 89 1191
Size: 89 1102
PpuMI rG'GwC_Cy
Cuts at: 0 93 1191
Size: 93 1098
PstI C_TGCA'G
Cuts at: 0 777 1191
Size: 777 414
PvuII CAG'CTG
Cuts at: 0 490 778 976 1191
Size: 490 288 198 215
Fragments arranged by size:
490 288 215 198
SfcI C'TryA_G
Cuts at: 0 773 1043 1191
Size: 773 270 148
Fragments arranged by size:
773 270 148
SmlI C'TyrA_G
Cuts at: 0 390 1191
Size: 390 801
SspI AAT'ATT
Cuts at: 0 1029 1191
Size: 1029 162
StuI AGG'CCT
Cuts at: 0 284 1191
Size: 284 907
StyI C'CwwG_G
Cuts at: 0 669 1049 1191
Size: 669 380 142
Fragments arranged by size:
669 380 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 847 1191
Size: 214 633 344
Fragments arranged by size:
633 344 214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 126 158 1191
Size: 126 32 1033
Fragments arranged by size:
1033 126 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 271 1191
Size: 271 920
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApoI BanI BanII
BfrBI BglI BplI BpmI Bpu10I BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspEI BspMI
BsrDI BstXI BstYI BstZ17I BtgI EaeI EarI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII MscI MspA1I NcoI
NdeI NsiI NspI PciI PflMI PfoI PpuMI PstI
PvuII SfcI SmlI SspI StuI StyI TaqII TstI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BmtI BsaI
BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TatI Tth111I XbaI XhoI
XmaI XmnI ZraI