Restriction map for pF1KB3491
 (Linear) (Six-base) MAPSORT of: KIBB3491.seq  Check: 1828  from: 1  to: 2034



KIBB3491.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1573    1629    2034 
   Size:       1573      56     405

  Fragments arranged by size: 

                1573     405      56
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     824     904    2034 
   Size:        824      80    1130

  Fragments arranged by size: 

                1130     824      80
 
AfeI AGC'GCT

Cuts at:      0     124    2034 
   Size:        124    1910
 
AflIII A'CryG_T

Cuts at:      0    1542    2034 
   Size:       1542     492
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1393    2034 
   Size:       1393     641
 
AleI CACnn'nnGTG

Cuts at:      0    1027    1907    2034 
   Size:       1027     880     127

  Fragments arranged by size: 

                1027     880     127
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1661    2034 
   Size:       1661     373
 
AlwNI CAG_nnn'CTG

Cuts at:      0     468     616    1006    2034 
   Size:        468     148     390    1028

  Fragments arranged by size: 

                1028     468     390     148
 
ApaI G_GGCC'C

Cuts at:      0    1718    2034 
   Size:       1718     316
 
ApoI r'AATT_y

Cuts at:      0     338     391     445     754    1246    1427    1954    1975 
   Size:        338      53      54     309     492     181     527      21

Cuts at:   1975    2034 
   Size:         59

  Fragments arranged by size: 

                 527     492     338     309     181      59      54      53
                  21
 
AvaI C'yCGr_G

Cuts at:      0    1745    2034 
   Size:       1745     289
 
BamHI G'GATC_C

Cuts at:      0      33    2034 
   Size:         33    2001
 
BanI G'GyrC_C

Cuts at:      0    1447    2034 
   Size:       1447     587
 
BanII G_rGCy'C

Cuts at:      0    1000    1718    2034 
   Size:       1000     718     316

  Fragments arranged by size: 

                1000     718     316
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     116    1057    2034 
   Size:        116     941     977

  Fragments arranged by size: 

                 977     941     116
 
BbvCI CC'TCA_GC

Cuts at:      0     887    1719    2034 
   Size:        887     832     315

  Fragments arranged by size: 

                 887     832     315
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     650     684    2034 
   Size:        650      34    1350

  Fragments arranged by size: 

                1350     650      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1043    1906    2034 
   Size:       1043     863     128

  Fragments arranged by size: 

                1043     863     128
 
BclI T'GATC_A

Cuts at:      0     192    1899    2034 
   Size:        192    1707     135

  Fragments arranged by size: 

                1707     192     135
 
BfrBI ATG'CAT

Cuts at:      0     661    2034 
   Size:        661    1373
 
BglI GCCn_nnn'nGGC

Cuts at:      0     883     957    2034 
   Size:        883      74    1077

  Fragments arranged by size: 

                1077     883      74
 
BglII A'GATC_T

Cuts at:      0     568    1041    2034 
   Size:        568     473     993

  Fragments arranged by size: 

                 993     568     473
 
Bme1580I G_kGCm'C

Cuts at:      0    1718    2034 
   Size:       1718     316
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     390    1761    2034 
   Size:        390    1371     273

  Fragments arranged by size: 

                1371     390     273
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     445     638     946    1028    1184    1316    1328 
   Size:        242     203     193     308      82     156     132      12

Cuts at:   1328    1391    1592    1786    2034 
   Size:         63     201     194     248

  Fragments arranged by size: 

                 308     248     242     203     201     194     193     156
                 132      82      63      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     887    1719    2034 
   Size:        887     832     315

  Fragments arranged by size: 

                 887     832     315
 
BsaAI yAC'GTr

Cuts at:      0    1545    2034 
   Size:       1545     489
 
BsaWI w'CCGG_w

Cuts at:      0     419    2034 
   Size:        419    1615
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1087    1117    2034 
   Size:       1087      30     917

  Fragments arranged by size: 

                1087     917      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     610    2034 
   Size:        610    1424
 
BseYI C'CCAG_C

Cuts at:      0    1590    2034 
   Size:       1590     444
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     190    2034 
   Size:         34     156    1844

  Fragments arranged by size: 

                1844     156      34
 
BsiHKAI G_wGCw'C

Cuts at:      0     258    1415    2034 
   Size:        258    1157     619

  Fragments arranged by size: 

                1157     619     258
 
BsmI GAATG_Cn'

Cuts at:      0    1064    2034 
   Size:       1064     970
 
Bsp1286I G_dGCh'C

Cuts at:      0     258    1000    1415    1718    2034 
   Size:        258     742     415     303     316

  Fragments arranged by size: 

                 742     415     316     303     258
 
BspHI T'CATG_A

Cuts at:      0      39    2034 
   Size:         39    1995
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1573    1629    2034 
   Size:       1573      56     405

  Fragments arranged by size: 

                1573     405      56
 
BsrDI GCAATG_nn'

Cuts at:      0    1509    1871    2034 
   Size:       1509     362     163

  Fragments arranged by size: 

                1509     362     163
 
BsrGI T'GTAC_A

Cuts at:      0    1103    2034 
   Size:       1103     931
 
BstEII G'GTnAC_C

Cuts at:      0     558    1605    2034 
   Size:        558    1047     429

  Fragments arranged by size: 

                1047     558     429
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1338    2034 
   Size:       1338     696
 
BstYI r'GATC_y

Cuts at:      0      33     568    1041    2034 
   Size:         33     535     473     993

  Fragments arranged by size: 

                 993     535     473      33
 
BtgI C'CryG_G

Cuts at:      0    1382    1577    2034 
   Size:       1382     195     457

  Fragments arranged by size: 

                1382     457     195
 
BtsI GCAGTG_nn'

Cuts at:      0    1022    1034    2024    2034 
   Size:       1022      12     990      10

  Fragments arranged by size: 

                1022     990      12      10
 
DraI TTT'AAA

Cuts at:      0    1556    2034 
   Size:       1556     478
 
EaeI y'GGCC_r

Cuts at:      0    1656    2034 
   Size:       1656     378
 
EarI CTCTTCn'nnn_

Cuts at:      0    1251    2034 
   Size:       1251     783
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     445     638     824     904     946    1028    1184 
   Size:        242     203     193     186      80      42      82     156

Cuts at:   1184    1316    1328    1391    1592    1786    2034 
   Size:        132      12      63     201     194     248

  Fragments arranged by size: 

                 248     242     203     201     194     193     186     156
                 132      82      80      63      42      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     941    2034 
   Size:        941    1093
 
EcoO109I rG'GnC_Cy

Cuts at:      0     893    1387    1714    1715    2034 
   Size:        893     494     327       1     319

  Fragments arranged by size: 

                 893     494     327     319       1
 
EcoRI G'AATT_C

Cuts at:      0     445    2034 
   Size:        445    1589
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1152    2034 
   Size:       1152     882
 
HaeII r_GCGC'y

Cuts at:      0     126     849    1403    2034 
   Size:        126     723     554     631

  Fragments arranged by size: 

                 723     631     554     126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     204     236     519     551     611     643    1379    1411 
   Size:        204      32     283      32      60      32     736      32

Cuts at:   1411    1728    1760    1904    1936    2034 
   Size:        317      32     144      32      98

  Fragments arranged by size: 

                 736     317     283     204     144      98      60      32
                  32      32      32      32      32
 
MfeI C'AATT_G

Cuts at:      0     472    2034 
   Size:        472    1562
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     423    2034 
   Size:        423    1611
 
MscI TGG'CCA

Cuts at:      0    1658    2034 
   Size:       1658     376
 
MslI CAynn'nnrTG

Cuts at:      0    1027    1907    2034 
   Size:       1027     880     127

  Fragments arranged by size: 

                1027     880     127
 
MspA1I CmG'CkG

Cuts at:      0     616    1570    1594    1806    1843    2034 
   Size:        616     954      24     212      37     191

  Fragments arranged by size: 

                 954     616     212     191      37      24
 
NcoI C'CATG_G

Cuts at:      0    1577    2034 
   Size:       1577     457
 
NsiI A_TGCA'T

Cuts at:      0     663    2034 
   Size:        663    1371
 
NspI r_CATG'y

Cuts at:      0     484     579    2034 
   Size:        484      95    1455

  Fragments arranged by size: 

                1455     484      95
 
PfoI T'CCnGG_A

Cuts at:      0     461    1415    1703    2034 
   Size:        461     954     288     331

  Fragments arranged by size: 

                 954     461     331     288
 
PmlI CAC'GTG

Cuts at:      0    1545    2034 
   Size:       1545     489
 
PpuMI rG'GwC_Cy

Cuts at:      0    1387    2034 
   Size:       1387     647
 
PshAI GACnn'nnGTC

Cuts at:      0     930    2034 
   Size:        930    1104
 
PspOMI G'GGCC_C

Cuts at:      0    1714    2034 
   Size:       1714     320
 
PspXI vC'TCGA_Gb

Cuts at:      0    1745    2034 
   Size:       1745     289
 
PstI C_TGCA'G

Cuts at:      0    1029    1640    1743    2034 
   Size:       1029     611     103     291

  Fragments arranged by size: 

                1029     611     291     103
 
PvuII CAG'CTG

Cuts at:      0     616    1570    1594    2034 
   Size:        616     954      24     440

  Fragments arranged by size: 

                 954     616     440      24
 
SanDI GG'GwC_CC

Cuts at:      0    1387    2034 
   Size:       1387     647
 
ScaI AGT'ACT

Cuts at:      0      75    2034 
   Size:         75    1959
 
SexAI A'CCwGG_T

Cuts at:      0     635    2034 
   Size:        635    1399
 
SfcI C'TryA_G

Cuts at:      0      61    1025    1037    1164    1636    1739    2034 
   Size:         61     964      12     127     472     103     295

  Fragments arranged by size: 

                 964     472     295     127     103      61      12
 
SmlI C'TyrA_G

Cuts at:      0    1745    2034 
   Size:       1745     289
 
StuI AGG'CCT

Cuts at:      0     951    1832    2034 
   Size:        951     881     202

  Fragments arranged by size: 

                 951     881     202
 
StyI C'CwwG_G

Cuts at:      0     200    1577    1781    2034 
   Size:        200    1377     204     253

  Fragments arranged by size: 

                1377     253     204     200
 
TatI w'GTAC_w

Cuts at:      0      73    1103    2034 
   Size:         73    1030     931

  Fragments arranged by size: 

                1030     931      73
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     273    1075    2034 
   Size:        273     802     959

  Fragments arranged by size: 

                 959     802     273
 
XhoI C'TCGA_G

Cuts at:      0    1745    2034 
   Size:       1745     289
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApaI     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI   BstEII
    BstXI    BstYI     BtgI     BtsI     DraI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PfoI     PmlI
    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII    SanDI     ScaI
    SexAI     SfcI     SmlI     StuI     StyI     TatI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AloI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BlpI    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI
     PpiI     PsiI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I
     XbaI     XmaI     XmnI     ZraI
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