Restriction map for
pF1KB3489
(Linear) (Six-base) MAPSORT of: KIBB3489.seq Check: 442 from: 1 to: 996
KIBB3489.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 867 996
Size: 867 129
AccI GT'mk_AC
Cuts at: 0 626 996
Size: 626 370
AleI CACnn'nnGTG
Cuts at: 0 727 996
Size: 727 269
ApoI r'AATT_y
Cuts at: 0 385 783 996
Size: 385 398 213
Fragments arranged by size:
398 385 213
AseI AT'TA_AT
Cuts at: 0 242 996
Size: 242 754
AvaI C'yCGr_G
Cuts at: 0 91 996
Size: 91 905
BanII G_rGCy'C
Cuts at: 0 872 996
Size: 872 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 129 163 996
Size: 129 34 833
Fragments arranged by size:
833 129 34
BclI T'GATC_A
Cuts at: 0 467 996
Size: 467 529
BglI GCCn_nnn'nGGC
Cuts at: 0 494 996
Size: 494 502
BglII A'GATC_T
Cuts at: 0 393 996
Size: 393 603
BmrI ACTGGGnnnn_n'
Cuts at: 0 343 996
Size: 343 653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 856 996
Size: 830 26 140
Fragments arranged by size:
830 140 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 996
Size: 269 727
BsaI GGTCTCn'nnnn_
Cuts at: 0 712 996
Size: 712 284
BseYI C'CCAG_C
Cuts at: 0 454 996
Size: 454 542
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 996
Size: 383 613
Bsp1286I G_dGCh'C
Cuts at: 0 872 996
Size: 872 124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 867 996
Size: 867 129
BsrBI CCG'CTC
Cuts at: 0 63 996
Size: 63 933
BsrDI GCAATG_nn'
Cuts at: 0 140 996
Size: 140 856
BsrGI T'GTAC_A
Cuts at: 0 47 996
Size: 47 949
BssSI C'ACGA_G
Cuts at: 0 25 996
Size: 25 971
BstEII G'GTnAC_C
Cuts at: 0 882 996
Size: 882 114
BstYI r'GATC_y
Cuts at: 0 393 996
Size: 393 603
BtgI C'CryG_G
Cuts at: 0 887 996
Size: 887 109
BtsI GCAGTG_nn'
Cuts at: 0 380 996
Size: 380 616
EaeI y'GGCC_r
Cuts at: 0 749 996
Size: 749 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 856 996
Size: 830 26 140
Fragments arranged by size:
830 140 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 414 996
Size: 414 582
HaeII r_GCGC'y
Cuts at: 0 489 996
Size: 489 507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 996
Size: 709 32 255
Fragments arranged by size:
709 255 32
HindIII A'AGCT_T
Cuts at: 0 593 996
Size: 593 403
MscI TGG'CCA
Cuts at: 0 751 996
Size: 751 245
MslI CAynn'nnrTG
Cuts at: 0 475 727 729 996
Size: 475 252 2 267
Fragments arranged by size:
475 267 252 2
NcoI C'CATG_G
Cuts at: 0 887 996
Size: 887 109
NspI r_CATG'y
Cuts at: 0 103 351 819 996
Size: 103 248 468 177
Fragments arranged by size:
468 248 177 103
PflMI CCAn_nnn'nTGG
Cuts at: 0 879 996
Size: 879 117
PsiI TTA'TAA
Cuts at: 0 175 996
Size: 175 821
PstI C_TGCA'G
Cuts at: 0 212 996
Size: 212 784
SfcI C'TryA_G
Cuts at: 0 208 996
Size: 208 788
SmaI CCC'GGG
Cuts at: 0 93 996
Size: 93 903
SmlI C'TyrA_G
Cuts at: 0 284 996
Size: 284 712
StyI C'CwwG_G
Cuts at: 0 798 887 921 996
Size: 798 89 34 75
Fragments arranged by size:
798 89 75 34
TatI w'GTAC_w
Cuts at: 0 47 996
Size: 47 949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 359 391 996
Size: 359 32 605
Fragments arranged by size:
605 359 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 582 996
Size: 582 414
XmaI C'CCGG_G
Cuts at: 0 91 996
Size: 91 905
Enzymes that do cut:
AarI AccI AleI ApoI AseI AvaI BanII BcgI
BclI BglI BglII BmrI BpmI BpuEI BsaI BseYI
BsgI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstEII
BstYI BtgI BtsI EaeI Eco57MI EcoNI HaeII Hin4I
HindIII MscI MslI NcoI NspI PflMI PsiI PstI
SfcI SmaI SmlI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI
BfrBI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BssHII BstAPI BstBI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmnI ZraI