Restriction map for
pF1KB3479
(Linear) (Six-base) MAPSORT of: KIBB3479.seq Check: 5037 from: 1 to: 1263
KIBB3479.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 817 1263
Size: 817 446
AccI GT'mk_AC
Cuts at: 0 214 937 1263
Size: 214 723 326
Fragments arranged by size:
723 326 214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 479 1263
Size: 479 784
AlwNI CAG_nnn'CTG
Cuts at: 0 295 809 1263
Size: 295 514 454
Fragments arranged by size:
514 454 295
ApoI r'AATT_y
Cuts at: 0 248 1263
Size: 248 1015
BanI G'GyrC_C
Cuts at: 0 782 962 1263
Size: 782 180 301
Fragments arranged by size:
782 301 180
BanII G_rGCy'C
Cuts at: 0 60 342 1263
Size: 60 282 921
Fragments arranged by size:
921 282 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 1263
Size: 209 1054
BfrBI ATG'CAT
Cuts at: 0 1150 1263
Size: 1150 113
BglI GCCn_nnn'nGGC
Cuts at: 0 760 1263
Size: 760 503
BlpI GC'TnA_GC
Cuts at: 0 680 1263
Size: 680 583
Bme1580I G_kGCm'C
Cuts at: 0 429 1263
Size: 429 834
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 572 1263
Size: 572 691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 647 1263
Size: 129 518 616
Fragments arranged by size:
616 518 129
Bpu10I CC'TnA_GC
Cuts at: 0 33 1263
Size: 33 1230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 1263
Size: 183 1080
BsaAI yAC'GTr
Cuts at: 0 1020 1263
Size: 1020 243
BsaWI w'CCGG_w
Cuts at: 0 434 1263
Size: 434 829
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 332 1263
Size: 332 931
BseYI C'CCAG_C
Cuts at: 0 645 741 786 1006 1263
Size: 645 96 45 220 257
Fragments arranged by size:
645 257 220 96 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 449 1135 1263
Size: 85 364 686 128
Fragments arranged by size:
686 364 128 85
BsiHKAI G_wGCw'C
Cuts at: 0 686 1174 1263
Size: 686 488 89
Fragments arranged by size:
686 488 89
BsmI GAATG_Cn'
Cuts at: 0 998 1263
Size: 998 265
Bsp1286I G_dGCh'C
Cuts at: 0 60 342 429 686 1174 1263
Size: 60 282 87 257 488 89
Fragments arranged by size:
488 282 257 89 87 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 817 1263
Size: 817 446
BsrDI GCAATG_nn'
Cuts at: 0 174 273 936 1263
Size: 174 99 663 327
Fragments arranged by size:
663 327 174 99
BssSI C'ACGA_G
Cuts at: 0 40 1263
Size: 40 1223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 378 809 1052 1187 1263
Size: 378 431 243 135 76
Fragments arranged by size:
431 378 243 135 76
BstBI TT'CG_AA
Cuts at: 0 95 1263
Size: 95 1168
BstEII G'GTnAC_C
Cuts at: 0 736 1263
Size: 736 527
BstZ17I GTA'TAC
Cuts at: 0 215 938 1263
Size: 215 723 325
Fragments arranged by size:
723 325 215
BtgI C'CryG_G
Cuts at: 0 662 1263
Size: 662 601
BtsI GCAGTG_nn'
Cuts at: 0 659 1263
Size: 659 604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 207 242 1263
Size: 207 35 1021
Fragments arranged by size:
1021 207 35
DraI TTT'AAA
Cuts at: 0 68 1263
Size: 68 1195
DraIII CAC_nnn'GTG
Cuts at: 0 1074 1160 1263
Size: 1074 86 103
Fragments arranged by size:
1074 103 86
EaeI y'GGCC_r
Cuts at: 0 138 365 1263
Size: 138 227 898
Fragments arranged by size:
898 227 138
EarI CTCTTCn'nnn_
Cuts at: 0 140 167 1246 1263
Size: 140 27 1079 17
Fragments arranged by size:
1079 140 27 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 647 1263
Size: 129 518 616
Fragments arranged by size:
616 518 129
EcoO109I rG'GnC_Cy
Cuts at: 0 477 604 1263
Size: 477 127 659
Fragments arranged by size:
659 477 127
EcoRI G'AATT_C
Cuts at: 0 248 1263
Size: 248 1015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 48 1263
Size: 48 1215
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 1263
Size: 658 32 573
Fragments arranged by size:
658 573 32
HincII GTy'rAC
Cuts at: 0 828 1263
Size: 828 435
MscI TGG'CCA
Cuts at: 0 140 367 1263
Size: 140 227 896
Fragments arranged by size:
896 227 140
MslI CAynn'nnrTG
Cuts at: 0 382 878 1263
Size: 382 496 385
Fragments arranged by size:
496 385 382
MspA1I CmG'CkG
Cuts at: 0 447 1142 1263
Size: 447 695 121
Fragments arranged by size:
695 447 121
NcoI C'CATG_G
Cuts at: 0 662 1263
Size: 662 601
NsiI A_TGCA'T
Cuts at: 0 1152 1263
Size: 1152 111
NspI r_CATG'y
Cuts at: 0 381 1150 1263
Size: 381 769 113
Fragments arranged by size:
769 381 113
PfoI T'CCnGG_A
Cuts at: 0 624 1263
Size: 624 639
PpuMI rG'GwC_Cy
Cuts at: 0 477 1263
Size: 477 786
PstI C_TGCA'G
Cuts at: 0 805 1183 1263
Size: 805 378 80
Fragments arranged by size:
805 378 80
PvuII CAG'CTG
Cuts at: 0 447 1142 1263
Size: 447 695 121
Fragments arranged by size:
695 447 121
SapI GCTCTTCn'nnn_
Cuts at: 0 140 1263
Size: 140 1123
SfcI C'TryA_G
Cuts at: 0 801 1179 1263
Size: 801 378 84
Fragments arranged by size:
801 378 84
SmlI C'TyrA_G
Cuts at: 0 162 1263
Size: 162 1101
SphI G_CATG'C
Cuts at: 0 1150 1263
Size: 1150 113
SspI AAT'ATT
Cuts at: 0 700 1263
Size: 700 563
StyI C'CwwG_G
Cuts at: 0 392 600 662 1263
Size: 392 208 62 601
Fragments arranged by size:
601 392 208 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 800 1263
Size: 800 463
TatI w'GTAC_w
Cuts at: 0 374 1263
Size: 374 889
XmnI GAAnn'nnTTC
Cuts at: 0 973 1263
Size: 973 290
Enzymes that do cut:
AarI AccI AlfI AlwNI ApoI BanI BanII BbsI
BfrBI BglI BlpI Bme1580I BplI BpmI Bpu10I BpuEI
BsaAI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtsI CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I
EcoRI FalI Hin4I HincII MscI MslI MspA1I NcoI
NsiI NspI PfoI PpuMI PstI PvuII SapI SfcI
SmlI SphI SspI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BglII BmgBI BmrI BmtI BsaI BsaBI BsaHI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BstXI BstYI Bsu36I BtgZI ClaI DrdI
EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI