Restriction map for
pF1KB3474
(Linear) (Six-base) MAPSORT of: KIBB3474.seq Check: 3611 from: 1 to: 591
KIBB3474.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AleI CACnn'nnGTG
Cuts at: 0 187 591
Size: 187 404
AvaI C'yCGr_G
Cuts at: 0 287 297 591
Size: 287 10 294
Fragments arranged by size:
294 287 10
BamHI G'GATC_C
Cuts at: 0 433 591
Size: 433 158
BanII G_rGCy'C
Cuts at: 0 350 568 583 591
Size: 350 218 15 8
Fragments arranged by size:
350 218 15 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 371 591
Size: 371 220
BglI GCCn_nnn'nGGC
Cuts at: 0 40 591
Size: 40 551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 591
Size: 334 257
BsaI GGTCTCn'nnnn_
Cuts at: 0 408 591
Size: 408 183
BsaHI Gr'CG_yC
Cuts at: 0 403 591
Size: 403 188
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 83 162 591
Size: 83 79 429
Fragments arranged by size:
429 83 79
BseYI C'CCAG_C
Cuts at: 0 243 591
Size: 243 348
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 591
Size: 392 199
BsiHKAI G_wGCw'C
Cuts at: 0 350 583 591
Size: 350 233 8
Fragments arranged by size:
350 233 8
BsmI GAATG_Cn'
Cuts at: 0 228 591
Size: 228 363
Bsp1286I G_dGCh'C
Cuts at: 0 350 568 583 591
Size: 350 218 15 8
Fragments arranged by size:
350 218 15 8
BstXI CCAn_nnnn'nTGG
Cuts at: 0 163 444 591
Size: 163 281 147
Fragments arranged by size:
281 163 147
BstYI r'GATC_y
Cuts at: 0 433 591
Size: 433 158
BtgI C'CryG_G
Cuts at: 0 461 591
Size: 461 130
EaeI y'GGCC_r
Cuts at: 0 446 591
Size: 446 145
EarI CTCTTCn'nnn_
Cuts at: 0 406 591
Size: 406 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 591
Size: 265 326
EcoICRI GAG'CTC
Cuts at: 0 348 581 591
Size: 348 233 10
Fragments arranged by size:
348 233 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 591
Size: 334 257
EcoNI CCTnn'n_nnAGG
Cuts at: 0 423 591
Size: 423 168
EcoO109I rG'GnC_Cy
Cuts at: 0 167 329 591
Size: 167 162 262
Fragments arranged by size:
262 167 162
HaeII r_GCGC'y
Cuts at: 0 531 591
Size: 531 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 316 348 591
Size: 159 32 125 32 243
Fragments arranged by size:
243 159 125 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 591
Size: 365 226
MscI TGG'CCA
Cuts at: 0 448 591
Size: 448 143
MslI CAynn'nnrTG
Cuts at: 0 187 591
Size: 187 404
MspA1I CmG'CkG
Cuts at: 0 187 211 247 591
Size: 187 24 36 344
Fragments arranged by size:
344 187 36 24
NcoI C'CATG_G
Cuts at: 0 461 591
Size: 461 130
PasI CC'CwG_GG
Cuts at: 0 253 591
Size: 253 338
PpuMI rG'GwC_Cy
Cuts at: 0 167 591
Size: 167 424
PvuII CAG'CTG
Cuts at: 0 247 591
Size: 247 344
SacI G_AGCT'C
Cuts at: 0 350 583 591
Size: 350 233 8
Fragments arranged by size:
350 233 8
SmaI CCC'GGG
Cuts at: 0 299 591
Size: 299 292
StyI C'CwwG_G
Cuts at: 0 461 490 591
Size: 461 29 101
Fragments arranged by size:
461 101 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 257 591
Size: 85 172 334
Fragments arranged by size:
334 172 85
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 475 591
Size: 475 116
XmaI C'CCGG_G
Cuts at: 0 297 591
Size: 297 294
Enzymes that do cut:
AleI AvaI BamHI BanII BbsI BglI BpmI BsaI
BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BstXI
BstYI BtgI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NcoI
PasI PpuMI PvuII SacI SmaI StyI TaqII XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmnI ZraI