(Linear) (Six-base) MAPSORT of: KIBB3469.seq  Check: 1644  from: 1  to: 573



KIBB3469.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     136     573 
   Size:         23     113     437

  Fragments arranged by size: 

                 437     113      23
 
ApoI r'AATT_y

Cuts at:      0     193     573 
   Size:        193     380
 
AvaI C'yCGr_G

Cuts at:      0     341     573 
   Size:        341     232
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     318     352     573 
   Size:        318      34     221

  Fragments arranged by size: 

                 318     221      34
 
BfrBI ATG'CAT

Cuts at:      0     526     573 
   Size:        526      47
 
Bpu10I CC'TnA_GC

Cuts at:      0     179     573 
   Size:        179     394
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64      67     151     573 
   Size:         64       3      84     422

  Fragments arranged by size: 

                 422      84      64       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     143     573 
   Size:        143     430
 
Bsp1286I G_dGCh'C

Cuts at:      0     143     573 
   Size:        143     430
 
BsrDI GCAATG_nn'

Cuts at:      0     336     573 
   Size:        336     237
 
Bsu36I CC'TnA_GG

Cuts at:      0     533     573 
   Size:        533      40
 
EarI CTCTTCn'nnn_

Cuts at:      0     500     573 
   Size:        500      73
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     136     573 
   Size:         23     113     437

  Fragments arranged by size: 

                 437     113      23
 
EcoRV GAT'ATC

Cuts at:      0     336     573 
   Size:        336     237
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     289     321     573 
   Size:        289      32     252

  Fragments arranged by size: 

                 289     252      32
 
MspA1I CmG'CkG

Cuts at:      0      25     573 
   Size:         25     548
 
NsiI A_TGCA'T

Cuts at:      0     528     573 
   Size:        528      45
 
NspI r_CATG'y

Cuts at:      0     526     573 
   Size:        526      47
 
PasI CC'CwG_GG

Cuts at:      0     242     573 
   Size:        242     331
 
PvuII CAG'CTG

Cuts at:      0      25     573 
   Size:         25     548
 
SexAI A'CCwGG_T

Cuts at:      0     446     573 
   Size:        446     127
 
SphI G_CATG'C

Cuts at:      0     526     573 
   Size:        526      47
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BcgI    BfrBI   Bpu10I    BseRI  BsiHKAI
 Bsp1286I    BsrDI   Bsu36I     EarI  Eco57MI    EcoRV    Hin4I   MspA1I
     NsiI     NspI     PasI    PvuII    SexAI     SphI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI