Restriction map for
pF1KB3465
(Linear) (Six-base) MAPSORT of: KIBB3465.seq Check: 4979 from: 1 to: 1035
KIBB3465.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 656 1035
Size: 265 391 379
Fragments arranged by size:
391 379 265
AleI CACnn'nnGTG
Cuts at: 0 782 1035
Size: 782 253
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 652 684 1035
Size: 652 32 351
Fragments arranged by size:
652 351 32
AlwNI CAG_nnn'CTG
Cuts at: 0 22 1035
Size: 22 1013
ApaLI G'TGCA_C
Cuts at: 0 888 1035
Size: 888 147
ApoI r'AATT_y
Cuts at: 0 228 522 1035
Size: 228 294 513
Fragments arranged by size:
513 294 228
AvaI C'yCGr_G
Cuts at: 0 414 1035
Size: 414 621
BamHI G'GATC_C
Cuts at: 0 419 1035
Size: 419 616
BanII G_rGCy'C
Cuts at: 0 415 1035
Size: 415 620
BciVI GTATCCnnnnn_n'
Cuts at: 0 747 767 1035
Size: 747 20 268
Fragments arranged by size:
747 268 20
Bme1580I G_kGCm'C
Cuts at: 0 892 967 1035
Size: 892 75 68
Fragments arranged by size:
892 75 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 10 1035
Size: 10 1025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 466 1035
Size: 317 149 569
Fragments arranged by size:
569 317 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 504 723 1035
Size: 504 219 312
Fragments arranged by size:
504 312 219
BsaBI GATnn'nnATC
Cuts at: 0 629 1035
Size: 629 406
BsaWI w'CCGG_w
Cuts at: 0 263 1035
Size: 263 772
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 228 526 1035
Size: 228 298 509
Fragments arranged by size:
509 298 228
BseYI C'CCAG_C
Cuts at: 0 223 1035
Size: 223 812
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 469 736 1035
Size: 361 108 267 299
Fragments arranged by size:
361 299 267 108
BsiHKAI G_wGCw'C
Cuts at: 0 892 1035
Size: 892 143
Bsp1286I G_dGCh'C
Cuts at: 0 415 892 967 1035
Size: 415 477 75 68
Fragments arranged by size:
477 415 75 68
BsrDI GCAATG_nn'
Cuts at: 0 121 1035
Size: 121 914
BsrFI r'CCGG_y
Cuts at: 0 438 1035
Size: 438 597
BsrGI T'GTAC_A
Cuts at: 0 467 809 874 1035
Size: 467 342 65 161
Fragments arranged by size:
467 342 161 65
BstYI r'GATC_y
Cuts at: 0 419 1035
Size: 419 616
BtgI C'CryG_G
Cuts at: 0 38 1035
Size: 38 997
EarI CTCTTCn'nnn_
Cuts at: 0 721 1035
Size: 721 314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 317 466 656 1035
Size: 265 52 149 190 379
Fragments arranged by size:
379 265 190 149 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 332 1035
Size: 332 703
EcoRI G'AATT_C
Cuts at: 0 228 1035
Size: 228 807
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 1035
Size: 34 32 969
Fragments arranged by size:
969 34 32
MslI CAynn'nnrTG
Cuts at: 0 782 1035
Size: 782 253
MspA1I CmG'CkG
Cuts at: 0 360 385 1035
Size: 360 25 650
Fragments arranged by size:
650 360 25
NcoI C'CATG_G
Cuts at: 0 38 1035
Size: 38 997
NdeI CA'TA_TG
Cuts at: 0 561 1035
Size: 561 474
NspI r_CATG'y
Cuts at: 0 291 540 1029 1035
Size: 291 249 489 6
Fragments arranged by size:
489 291 249 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 441 1035
Size: 441 594
PvuII CAG'CTG
Cuts at: 0 385 1035
Size: 385 650
SmaI CCC'GGG
Cuts at: 0 416 1035
Size: 416 619
SphI G_CATG'C
Cuts at: 0 291 540 1035
Size: 291 249 495
Fragments arranged by size:
495 291 249
StyI C'CwwG_G
Cuts at: 0 38 1035
Size: 38 997
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 696 896 1035
Size: 696 200 139
Fragments arranged by size:
696 200 139
TatI w'GTAC_w
Cuts at: 0 467 809 874 1035
Size: 467 342 65 161
Fragments arranged by size:
467 342 161 65
Tth111I GACn'n_nGTC
Cuts at: 0 46 1035
Size: 46 989
XmaI C'CCGG_G
Cuts at: 0 414 1035
Size: 414 621
Enzymes that do cut:
AcuI AleI AloI AlwNI ApaLI ApoI AvaI BamHI
BanII BciVI Bme1580I BmrI BpmI BsaI BsaBI BsaWI
BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BsrGI
BstYI BtgI EarI Eco57MI EcoNI EcoRI Hin4I MslI
MspA1I NcoI NdeI NspI PflMI PvuII SmaI SphI
StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TstI XbaI XcmI XhoI XmnI ZraI