Restriction map for
pF1KB3464
(Linear) (Six-base) MAPSORT of: KIBB3464.seq Check: 1926 from: 1 to: 864
KIBB3464.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 526 864
Size: 526 338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 864
Size: 727 137
AlwNI CAG_nnn'CTG
Cuts at: 0 765 864
Size: 765 99
BanI G'GyrC_C
Cuts at: 0 466 864
Size: 466 398
BanII G_rGCy'C
Cuts at: 0 699 864
Size: 699 165
BbsI GAAGACnn'nnnn_
Cuts at: 0 795 864
Size: 795 69
BfrBI ATG'CAT
Cuts at: 0 688 864
Size: 688 176
BglI GCCn_nnn'nGGC
Cuts at: 0 860 864
Size: 860 4
Bme1580I G_kGCm'C
Cuts at: 0 469 864
Size: 469 395
BmrI ACTGGGnnnn_n'
Cuts at: 0 62 864
Size: 62 802
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 807 864
Size: 807 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 864
Size: 592 272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 714 744 864
Size: 714 30 120
Fragments arranged by size:
714 120 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 236 302 864
Size: 236 66 562
Fragments arranged by size:
562 236 66
BseYI C'CCAG_C
Cuts at: 0 35 864
Size: 35 829
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 864
Size: 622 242
BsmI GAATG_Cn'
Cuts at: 0 387 690 864
Size: 387 303 174
Fragments arranged by size:
387 303 174
Bsp1286I G_dGCh'C
Cuts at: 0 469 699 864
Size: 469 230 165
Fragments arranged by size:
469 230 165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 764 864
Size: 764 100
BsrDI GCAATG_nn'
Cuts at: 0 773 864
Size: 773 91
BstYI r'GATC_y
Cuts at: 0 320 485 864
Size: 320 165 379
Fragments arranged by size:
379 320 165
Bsu36I CC'TnA_GG
Cuts at: 0 314 864
Size: 314 550
BtgI C'CryG_G
Cuts at: 0 713 864
Size: 713 151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 332 367 864
Size: 332 35 497
Fragments arranged by size:
497 332 35
EaeI y'GGCC_r
Cuts at: 0 2 864
Size: 2 862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 727 864
Size: 592 135 137
Fragments arranged by size:
592 137 135
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 864
Size: 328 536
EcoO109I rG'GnC_Cy
Cuts at: 0 75 232 864
Size: 75 157 632
Fragments arranged by size:
632 157 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 864
Size: 176 32 656
Fragments arranged by size:
656 176 32
MfeI C'AATT_G
Cuts at: 0 797 864
Size: 797 67
MslI CAynn'nnrTG
Cuts at: 0 277 820 864
Size: 277 543 44
Fragments arranged by size:
543 277 44
MspA1I CmG'CkG
Cuts at: 0 39 864
Size: 39 825
NcoI C'CATG_G
Cuts at: 0 713 864
Size: 713 151
NsiI A_TGCA'T
Cuts at: 0 690 864
Size: 690 174
NspI r_CATG'y
Cuts at: 0 522 864
Size: 522 342
PpuMI rG'GwC_Cy
Cuts at: 0 232 864
Size: 232 632
PsiI TTA'TAA
Cuts at: 0 130 169 864
Size: 130 39 695
Fragments arranged by size:
695 130 39
PstI C_TGCA'G
Cuts at: 0 627 738 761 864
Size: 627 111 23 103
Fragments arranged by size:
627 111 103 23
SexAI A'CCwGG_T
Cuts at: 0 234 864
Size: 234 630
SfcI C'TryA_G
Cuts at: 0 623 734 757 864
Size: 623 111 23 107
Fragments arranged by size:
623 111 107 23
StyI C'CwwG_G
Cuts at: 0 78 656 713 864
Size: 78 578 57 151
Fragments arranged by size:
578 151 78 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 548 580 714 746 864
Size: 548 32 134 32 118
Fragments arranged by size:
548 134 118 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 146 864
Size: 146 718
XmnI GAAnn'nnTTC
Cuts at: 0 294 864
Size: 294 570
Enzymes that do cut:
AccI AcuI AlwNI BanI BanII BbsI BfrBI BglI
Bme1580I BmrI BplI BpmI BsaXI BseRI BseYI BsgI
BsmI Bsp1286I BspMI BsrDI BstYI Bsu36I BtgI CspCI
EaeI Eco57MI EcoNI EcoO109I Hin4I MfeI MslI MspA1I
NcoI NsiI NspI PpuMI PsiI PstI SexAI SfcI
StyI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BglII BlpI BmgBI BmtI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI
ClaI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI ZraI