Restriction map for
pF1KB3456
(Linear) (Six-base) MAPSORT of: KIBB3456.seq Check: 7086 from: 1 to: 1077
KIBB3456.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AleI CACnn'nnGTG
Cuts at: 0 694 1077
Size: 694 383
AvaI C'yCGr_G
Cuts at: 0 845 1077
Size: 845 232
BanII G_rGCy'C
Cuts at: 0 258 357 462 1077
Size: 258 99 105 615
Fragments arranged by size:
615 258 105 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 1077
Size: 134 943
BclI T'GATC_A
Cuts at: 0 413 1077
Size: 413 664
BglI GCCn_nnn'nGGC
Cuts at: 0 402 1033 1077
Size: 402 631 44
Fragments arranged by size:
631 402 44
Bme1580I G_kGCm'C
Cuts at: 0 336 477 701 1077
Size: 336 141 224 376
Fragments arranged by size:
376 336 224 141
BmgBI CAC'GTC
Cuts at: 0 523 1077
Size: 523 554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 237 1077
Size: 57 180 840
Fragments arranged by size:
840 180 57
BsaAI yAC'GTr
Cuts at: 0 543 1077
Size: 543 534
BsaWI w'CCGG_w
Cuts at: 0 1004 1051 1077
Size: 1004 47 26
Fragments arranged by size:
1004 47 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 51 81 889 919 1077
Size: 51 30 808 30 158
Fragments arranged by size:
808 158 51 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 107 657 1077
Size: 107 550 420
Fragments arranged by size:
550 420 107
BseYI C'CCAG_C
Cuts at: 0 570 1077
Size: 570 507
BsiHKAI G_wGCw'C
Cuts at: 0 357 1077
Size: 357 720
BsmI GAATG_Cn'
Cuts at: 0 433 1077
Size: 433 644
BsmBI CGTCTCn'nnnn_
Cuts at: 0 100 1077
Size: 100 977
Bsp1286I G_dGCh'C
Cuts at: 0 258 336 357 462 477 701 1077
Size: 258 78 21 105 15 224 376
Fragments arranged by size:
376 258 224 105 78 21 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 292 1077
Size: 292 785
BsrBI CCG'CTC
Cuts at: 0 377 1077
Size: 377 700
BsrDI GCAATG_nn'
Cuts at: 0 416 1077
Size: 416 661
BsrFI r'CCGG_y
Cuts at: 0 3 1077
Size: 3 1074
BssSI C'ACGA_G
Cuts at: 0 461 1077
Size: 461 616
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 786 1077
Size: 429 357 291
Fragments arranged by size:
429 357 291
EaeI y'GGCC_r
Cuts at: 0 980 1034 1077
Size: 980 54 43
Fragments arranged by size:
980 54 43
EarI CTCTTCn'nnn_
Cuts at: 0 814 1077
Size: 814 263
EcoICRI GAG'CTC
Cuts at: 0 355 1077
Size: 355 722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 237 1077
Size: 57 180 840
Fragments arranged by size:
840 180 57
EcoO109I rG'GnC_Cy
Cuts at: 0 78 1077
Size: 78 999
EcoRV GAT'ATC
Cuts at: 0 27 817 1077
Size: 27 790 260
Fragments arranged by size:
790 260 27
FspI TGC'GCA
Cuts at: 0 562 1077
Size: 562 515
HaeII r_GCGC'y
Cuts at: 0 67 1077
Size: 67 1010
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 1077
Size: 13 32 1032
Fragments arranged by size:
1032 32 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1077
Size: 83 994
MscI TGG'CCA
Cuts at: 0 1036 1077
Size: 1036 41
MslI CAynn'nnrTG
Cuts at: 0 232 694 1077
Size: 232 462 383
Fragments arranged by size:
462 383 232
MspA1I CmG'CkG
Cuts at: 0 574 941 1077
Size: 574 367 136
Fragments arranged by size:
574 367 136
NaeI GCC'GGC
Cuts at: 0 5 1077
Size: 5 1072
NgoMIV G'CCGG_C
Cuts at: 0 3 1077
Size: 3 1074
NspI r_CATG'y
Cuts at: 0 502 1077
Size: 502 575
PasI CC'CwG_GG
Cuts at: 0 336 1077
Size: 336 741
PmlI CAC'GTG
Cuts at: 0 543 1077
Size: 543 534
PpuMI rG'GwC_Cy
Cuts at: 0 78 1077
Size: 78 999
PstI C_TGCA'G
Cuts at: 0 20 86 1077
Size: 20 66 991
Fragments arranged by size:
991 66 20
PvuII CAG'CTG
Cuts at: 0 574 1077
Size: 574 503
SacI G_AGCT'C
Cuts at: 0 357 1077
Size: 357 720
SfcI C'TryA_G
Cuts at: 0 16 82 1077
Size: 16 66 995
Fragments arranged by size:
995 66 16
SmaI CCC'GGG
Cuts at: 0 847 1077
Size: 847 230
StyI C'CwwG_G
Cuts at: 0 200 1019 1077
Size: 200 819 58
Fragments arranged by size:
819 200 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 584 868 979 1077
Size: 584 284 111 98
Fragments arranged by size:
584 284 111 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 261 293 1077
Size: 261 32 784
Fragments arranged by size:
784 261 32
XmaI C'CCGG_G
Cuts at: 0 845 1077
Size: 845 232
XmnI GAAnn'nnTTC
Cuts at: 0 722 1077
Size: 722 355
Enzymes that do cut:
AleI AvaI BanII BbsI BclI BglI Bme1580I BmgBI
BpmI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstXI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FspI HaeII
Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NspI
PasI PmlI PpuMI PstI PvuII SacI SfcI SmaI
StyI TaqII TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsgI
BsiEI BsiWI BspEI BspHI BsrGI BssHII BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI ZraI