Restriction map for pF1KB3456
 (Linear) (Six-base) MAPSORT of: KIBB3456.seq  Check: 7086  from: 1  to: 1077



KIBB3456.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AleI CACnn'nnGTG

Cuts at:      0     694    1077 
   Size:        694     383
 
AvaI C'yCGr_G

Cuts at:      0     845    1077 
   Size:        845     232
 
BanII G_rGCy'C

Cuts at:      0     258     357     462    1077 
   Size:        258      99     105     615

  Fragments arranged by size: 

                 615     258     105      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     134    1077 
   Size:        134     943
 
BclI T'GATC_A

Cuts at:      0     413    1077 
   Size:        413     664
 
BglI GCCn_nnn'nGGC

Cuts at:      0     402    1033    1077 
   Size:        402     631      44

  Fragments arranged by size: 

                 631     402      44
 
Bme1580I G_kGCm'C

Cuts at:      0     336     477     701    1077 
   Size:        336     141     224     376

  Fragments arranged by size: 

                 376     336     224     141
 
BmgBI CAC'GTC

Cuts at:      0     523    1077 
   Size:        523     554
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     237    1077 
   Size:         57     180     840

  Fragments arranged by size: 

                 840     180      57
 
BsaAI yAC'GTr

Cuts at:      0     543    1077 
   Size:        543     534
 
BsaWI w'CCGG_w

Cuts at:      0    1004    1051    1077 
   Size:       1004      47      26

  Fragments arranged by size: 

                1004      47      26
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      51      81     889     919    1077 
   Size:         51      30     808      30     158

  Fragments arranged by size: 

                 808     158      51      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     107     657    1077 
   Size:        107     550     420

  Fragments arranged by size: 

                 550     420     107
 
BseYI C'CCAG_C

Cuts at:      0     570    1077 
   Size:        570     507
 
BsiHKAI G_wGCw'C

Cuts at:      0     357    1077 
   Size:        357     720
 
BsmI GAATG_Cn'

Cuts at:      0     433    1077 
   Size:        433     644
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     100    1077 
   Size:        100     977
 
Bsp1286I G_dGCh'C

Cuts at:      0     258     336     357     462     477     701    1077 
   Size:        258      78      21     105      15     224     376

  Fragments arranged by size: 

                 376     258     224     105      78      21      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     292    1077 
   Size:        292     785
 
BsrBI CCG'CTC

Cuts at:      0     377    1077 
   Size:        377     700
 
BsrDI GCAATG_nn'

Cuts at:      0     416    1077 
   Size:        416     661
 
BsrFI r'CCGG_y

Cuts at:      0       3    1077 
   Size:          3    1074
 
BssSI C'ACGA_G

Cuts at:      0     461    1077 
   Size:        461     616
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     429     786    1077 
   Size:        429     357     291

  Fragments arranged by size: 

                 429     357     291
 
EaeI y'GGCC_r

Cuts at:      0     980    1034    1077 
   Size:        980      54      43

  Fragments arranged by size: 

                 980      54      43
 
EarI CTCTTCn'nnn_

Cuts at:      0     814    1077 
   Size:        814     263
 
EcoICRI GAG'CTC

Cuts at:      0     355    1077 
   Size:        355     722
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     237    1077 
   Size:         57     180     840

  Fragments arranged by size: 

                 840     180      57
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78    1077 
   Size:         78     999
 
EcoRV GAT'ATC

Cuts at:      0      27     817    1077 
   Size:         27     790     260

  Fragments arranged by size: 

                 790     260      27
 
FspI TGC'GCA

Cuts at:      0     562    1077 
   Size:        562     515
 
HaeII r_GCGC'y

Cuts at:      0      67    1077 
   Size:         67    1010
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45    1077 
   Size:         13      32    1032

  Fragments arranged by size: 

                1032      32      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      83    1077 
   Size:         83     994
 
MscI TGG'CCA

Cuts at:      0    1036    1077 
   Size:       1036      41
 
MslI CAynn'nnrTG

Cuts at:      0     232     694    1077 
   Size:        232     462     383

  Fragments arranged by size: 

                 462     383     232
 
MspA1I CmG'CkG

Cuts at:      0     574     941    1077 
   Size:        574     367     136

  Fragments arranged by size: 

                 574     367     136
 
NaeI GCC'GGC

Cuts at:      0       5    1077 
   Size:          5    1072
 
NgoMIV G'CCGG_C

Cuts at:      0       3    1077 
   Size:          3    1074
 
NspI r_CATG'y

Cuts at:      0     502    1077 
   Size:        502     575
 
PasI CC'CwG_GG

Cuts at:      0     336    1077 
   Size:        336     741
 
PmlI CAC'GTG

Cuts at:      0     543    1077 
   Size:        543     534
 
PpuMI rG'GwC_Cy

Cuts at:      0      78    1077 
   Size:         78     999
 
PstI C_TGCA'G

Cuts at:      0      20      86    1077 
   Size:         20      66     991

  Fragments arranged by size: 

                 991      66      20
 
PvuII CAG'CTG

Cuts at:      0     574    1077 
   Size:        574     503
 
SacI G_AGCT'C

Cuts at:      0     357    1077 
   Size:        357     720
 
SfcI C'TryA_G

Cuts at:      0      16      82    1077 
   Size:         16      66     995

  Fragments arranged by size: 

                 995      66      16
 
SmaI CCC'GGG

Cuts at:      0     847    1077 
   Size:        847     230
 
StyI C'CwwG_G

Cuts at:      0     200    1019    1077 
   Size:        200     819      58

  Fragments arranged by size: 

                 819     200      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     584     868     979    1077 
   Size:        584     284     111      98

  Fragments arranged by size: 

                 584     284     111      98
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     261     293    1077 
   Size:        261      32     784

  Fragments arranged by size: 

                 784     261      32
 
XmaI C'CCGG_G

Cuts at:      0     845    1077 
   Size:        845     232
 
XmnI GAAnn'nnTTC

Cuts at:      0     722    1077 
   Size:        722     355
 

 Enzymes that do cut:

     AleI     AvaI    BanII     BbsI     BclI     BglI Bme1580I    BmgBI
     BpmI    BsaAI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI    BstXI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV     FspI    HaeII
    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI
     PasI     PmlI    PpuMI     PstI    PvuII     SacI     SfcI     SmaI
     StyI    TaqII     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI     BsgI
    BsiEI    BsiWI    BspEI    BspHI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     ZraI
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