(Linear) (Six-base) MAPSORT of: KIBB3456.seq Check: 7086 from: 1 to: 1077 KIBB3456.orf With 182 enzymes: SgfI * April 24, 2009 13:01 .. AleI CACnn'nnGTG Cuts at: 0 694 1077 Size: 694 383 AvaI C'yCGr_G Cuts at: 0 845 1077 Size: 845 232 BanII G_rGCy'C Cuts at: 0 258 357 462 1077 Size: 258 99 105 615 Fragments arranged by size: 615 258 105 99 BbsI GAAGACnn'nnnn_ Cuts at: 0 134 1077 Size: 134 943 BclI T'GATC_A Cuts at: 0 413 1077 Size: 413 664 BglI GCCn_nnn'nGGC Cuts at: 0 402 1033 1077 Size: 402 631 44 Fragments arranged by size: 631 402 44 Bme1580I G_kGCm'C Cuts at: 0 336 477 701 1077 Size: 336 141 224 376 Fragments arranged by size: 376 336 224 141 BmgBI CAC'GTC Cuts at: 0 523 1077 Size: 523 554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 237 1077 Size: 57 180 840 Fragments arranged by size: 840 180 57 BsaAI yAC'GTr Cuts at: 0 543 1077 Size: 543 534 BsaWI w'CCGG_w Cuts at: 0 1004 1051 1077 Size: 1004 47 26 Fragments arranged by size: 1004 47 26 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 51 81 889 919 1077 Size: 51 30 808 30 158 Fragments arranged by size: 808 158 51 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 107 657 1077 Size: 107 550 420 Fragments arranged by size: 550 420 107 BseYI C'CCAG_C Cuts at: 0 570 1077 Size: 570 507 BsiHKAI G_wGCw'C Cuts at: 0 357 1077 Size: 357 720 BsmI GAATG_Cn' Cuts at: 0 433 1077 Size: 433 644 BsmBI CGTCTCn'nnnn_ Cuts at: 0 100 1077 Size: 100 977 Bsp1286I G_dGCh'C Cuts at: 0 258 336 357 462 477 701 1077 Size: 258 78 21 105 15 224 376 Fragments arranged by size: 376 258 224 105 78 21 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 292 1077 Size: 292 785 BsrBI CCG'CTC Cuts at: 0 377 1077 Size: 377 700 BsrDI GCAATG_nn' Cuts at: 0 416 1077 Size: 416 661 BsrFI r'CCGG_y Cuts at: 0 3 1077 Size: 3 1074 BssSI C'ACGA_G Cuts at: 0 461 1077 Size: 461 616 BstXI CCAn_nnnn'nTGG Cuts at: 0 429 786 1077 Size: 429 357 291 Fragments arranged by size: 429 357 291 EaeI y'GGCC_r Cuts at: 0 980 1034 1077 Size: 980 54 43 Fragments arranged by size: 980 54 43 EarI CTCTTCn'nnn_ Cuts at: 0 814 1077 Size: 814 263 EcoICRI GAG'CTC Cuts at: 0 355 1077 Size: 355 722 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 237 1077 Size: 57 180 840 Fragments arranged by size: 840 180 57 EcoO109I rG'GnC_Cy Cuts at: 0 78 1077 Size: 78 999 EcoRV GAT'ATC Cuts at: 0 27 817 1077 Size: 27 790 260 Fragments arranged by size: 790 260 27 FspI TGC'GCA Cuts at: 0 562 1077 Size: 562 515 HaeII r_GCGC'y Cuts at: 0 67 1077 Size: 67 1010 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 13 45 1077 Size: 13 32 1032 Fragments arranged by size: 1032 32 13 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 83 1077 Size: 83 994 MscI TGG'CCA Cuts at: 0 1036 1077 Size: 1036 41 MslI CAynn'nnrTG Cuts at: 0 232 694 1077 Size: 232 462 383 Fragments arranged by size: 462 383 232 MspA1I CmG'CkG Cuts at: 0 574 941 1077 Size: 574 367 136 Fragments arranged by size: 574 367 136 NaeI GCC'GGC Cuts at: 0 5 1077 Size: 5 1072 NgoMIV G'CCGG_C Cuts at: 0 3 1077 Size: 3 1074 NspI r_CATG'y Cuts at: 0 502 1077 Size: 502 575 PasI CC'CwG_GG Cuts at: 0 336 1077 Size: 336 741 PmlI CAC'GTG Cuts at: 0 543 1077 Size: 543 534 PpuMI rG'GwC_Cy Cuts at: 0 78 1077 Size: 78 999 PstI C_TGCA'G Cuts at: 0 20 86 1077 Size: 20 66 991 Fragments arranged by size: 991 66 20 PvuII CAG'CTG Cuts at: 0 574 1077 Size: 574 503 SacI G_AGCT'C Cuts at: 0 357 1077 Size: 357 720 SfcI C'TryA_G Cuts at: 0 16 82 1077 Size: 16 66 995 Fragments arranged by size: 995 66 16 SmaI CCC'GGG Cuts at: 0 847 1077 Size: 847 230 StyI C'CwwG_G Cuts at: 0 200 1019 1077 Size: 200 819 58 Fragments arranged by size: 819 200 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 584 868 979 1077 Size: 584 284 111 98 Fragments arranged by size: 584 284 111 98 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 261 293 1077 Size: 261 32 784 Fragments arranged by size: 784 261 32 XmaI C'CCGG_G Cuts at: 0 845 1077 Size: 845 232 XmnI GAAnn'nnTTC Cuts at: 0 722 1077 Size: 722 355 Enzymes that do cut: AleI AvaI BanII BbsI BclI BglI Bme1580I BmgBI BpmI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstXI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PmlI PpuMI PstI PvuII SacI SfcI SmaI StyI TaqII TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsgI BsiEI BsiWI BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI ZraI