(Linear) (Six-base) MAPSORT of: KIBB3455.seq  Check: 4948  from: 1  to: 480



KIBB3455.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     480 
   Size:        458      22
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     314     480 
   Size:        314     166
 
ApaI G_GGCC'C

Cuts at:      0     183     480 
   Size:        183     297
 
ApaLI G'TGCA_C

Cuts at:      0     299     480 
   Size:        299     181
 
AvaI C'yCGr_G

Cuts at:      0     176     480 
   Size:        176     304
 
BanII G_rGCy'C

Cuts at:      0      78     183     412     446     480 
   Size:         78     105     229      34      34

  Fragments arranged by size: 

                 229     105      78      34      34
 
Bme1580I G_kGCm'C

Cuts at:      0     183     303     327     480 
   Size:        183     120      24     153

  Fragments arranged by size: 

                 183     153     120      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     325     402     480 
   Size:        304      21      77      78

  Fragments arranged by size: 

                 304      78      77      21
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     480 
   Size:        247     233
 
BsaWI w'CCGG_w

Cuts at:      0     464     480 
   Size:        464      16
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     331     361     480 
   Size:        331      30     119

  Fragments arranged by size: 

                 331     119      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     339     480 
   Size:        339     141
 
BsiHKAI G_wGCw'C

Cuts at:      0     303     480 
   Size:        303     177
 
BsmI GAATG_Cn'

Cuts at:      0     279     480 
   Size:        279     201
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     183     303     327     412     446     480 
   Size:         78     105     120      24      85      34      34

  Fragments arranged by size: 

                 120     105      85      78      34      34      24
 
BspEI T'CCGG_A

Cuts at:      0     464     480 
   Size:        464      16
 
EarI CTCTTCn'nnn_

Cuts at:      0      32     284     434     480 
   Size:         32     252     150      46

  Fragments arranged by size: 

                 252     150      46      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     325     402     458     480 
   Size:        304      21      77      56      22

  Fragments arranged by size: 

                 304      77      56      22      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     157     189     394     406     426     438     480 
   Size:        157      32     205      12      20      12      42

  Fragments arranged by size: 

                 205     157      42      32      20      12      12
 
MslI CAynn'nnrTG

Cuts at:      0      22      83     480 
   Size:         22      61     397

  Fragments arranged by size: 

                 397      61      22
 
PspOMI G'GGCC_C

Cuts at:      0     179     480 
   Size:        179     301
 
PstI C_TGCA'G

Cuts at:      0     335     480 
   Size:        335     145
 
SapI GCTCTTCn'nnn_

Cuts at:      0      32     284     434     480 
   Size:         32     252     150      46

  Fragments arranged by size: 

                 252     150      46      32
 
SfcI C'TryA_G

Cuts at:      0     331     480 
   Size:        331     149
 
SmaI CCC'GGG

Cuts at:      0     178     480 
   Size:        178     302
 
SmlI C'TyrA_G

Cuts at:      0     262     480 
   Size:        262     218
 
StyI C'CwwG_G

Cuts at:      0     159     377     480 
   Size:        159     218     103

  Fragments arranged by size: 

                 218     159     103
 
Tth111I GACn'n_nGTC

Cuts at:      0     145     480 
   Size:        145     335
 
XmaI C'CCGG_G

Cuts at:      0     176     480 
   Size:        176     304
 

 Enzymes that do cut:

     AcuI     AlfI     ApaI    ApaLI     AvaI    BanII Bme1580I     BpmI
    BpuEI    BsaWI    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BspEI
     EarI  Eco57MI    Hin4I     MslI   PspOMI     PstI     SapI     SfcI
     SmaI     SmlI     StyI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XhoI     XmnI     ZraI