(Linear) (Six-base) MAPSORT of: KIBB3451.seq  Check: 6124  from: 1  to: 792



KIBB3451.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     114     792 
   Size:        114     678
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     673     792 
   Size:        284     389     119

  Fragments arranged by size: 

                 389     284     119
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     761     792 
   Size:        761      31
 
ApoI r'AATT_y

Cuts at:      0     247     792 
   Size:        247     545
 
AvaI C'yCGr_G

Cuts at:      0     518     792 
   Size:        518     274
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     460     621     792 
   Size:        460     161     171

  Fragments arranged by size: 

                 460     171     161
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     721     755     792 
   Size:        721      34      37

  Fragments arranged by size: 

                 721      37      34
 
BlpI GC'TnA_GC

Cuts at:      0     467     792 
   Size:        467     325
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     734     792 
   Size:        734      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     792 
   Size:        169     623
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     187     792 
   Size:        187     605
 
BsaBI GATnn'nnATC

Cuts at:      0     508     792 
   Size:        508     284
 
BsaWI w'CCGG_w

Cuts at:      0     491     792 
   Size:        491     301
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547     792 
   Size:        547     245
 
BspEI T'CCGG_A

Cuts at:      0     491     792 
   Size:        491     301
 
BspHI T'CATG_A

Cuts at:      0     512     792 
   Size:        512     280
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     114     792 
   Size:        114     678
 
BstYI r'GATC_y

Cuts at:      0     309     792 
   Size:        309     483
 
BtgI C'CryG_G

Cuts at:      0     300     335     792 
   Size:        300      35     457

  Fragments arranged by size: 

                 457     300      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     472     792 
   Size:        472     320
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     673     792 
   Size:        284     389     119

  Fragments arranged by size: 

                 389     284     119
 
EcoO109I rG'GnC_Cy

Cuts at:      0     152     792 
   Size:        152     640
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     226     792 
   Size:        226     566
 
HaeII r_GCGC'y

Cuts at:      0      24     792 
   Size:         24     768
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     351     383     792 
   Size:        351      32     409

  Fragments arranged by size: 

                 409     351      32
 
MslI CAynn'nnrTG

Cuts at:      0     374     792 
   Size:        374     418
 
NcoI C'CATG_G

Cuts at:      0     300     335     792 
   Size:        300      35     457

  Fragments arranged by size: 

                 457     300      35
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     306     792 
   Size:        306     486
 
PpuMI rG'GwC_Cy

Cuts at:      0     152     792 
   Size:        152     640
 
PshAI GACnn'nnGTC

Cuts at:      0     142     792 
   Size:        142     650
 
SmlI C'TyrA_G

Cuts at:      0     184     792 
   Size:        184     608
 
SspI AAT'ATT

Cuts at:      0     129     792 
   Size:        129     663
 
StyI C'CwwG_G

Cuts at:      0     156     300     335     792 
   Size:        156     144      35     457

  Fragments arranged by size: 

                 457     156     144      35
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      57     669     792 
   Size:         57     612     123

  Fragments arranged by size: 

                 612     123      57
 
XmnI GAAnn'nnTTC

Cuts at:      0     269     792 
   Size:        269     523
 

 Enzymes that do cut:

     AarI     AcuI     AloI     ApoI     AvaI     BbsI     BcgI     BlpI
     BmrI    BpuEI     BsaI    BsaBI    BsaWI     BsgI    BspEI    BspHI
    BspMI    BstYI     BtgI     EciI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I     MslI     NcoI    PflMI    PpuMI    PshAI     SmlI     SspI
     StyI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BsaAI    BsaHI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI