Restriction map for
pF1KB3450
(Linear) (Six-base) MAPSORT of: KIBB3450.seq Check: 7252 from: 1 to: 879
KIBB3450.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 365 879
Size: 365 514
ApaI G_GGCC'C
Cuts at: 0 32 40 879
Size: 32 8 839
Fragments arranged by size:
839 32 8
AvaI C'yCGr_G
Cuts at: 0 31 677 879
Size: 31 646 202
Fragments arranged by size:
646 202 31
BanII G_rGCy'C
Cuts at: 0 32 40 150 879
Size: 32 8 110 729
Fragments arranged by size:
729 110 32 8
BbvCI CC'TCA_GC
Cuts at: 0 672 879
Size: 672 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 591 625 879
Size: 591 34 254
Fragments arranged by size:
591 254 34
BglI GCCn_nnn'nGGC
Cuts at: 0 44 563 879
Size: 44 519 316
Fragments arranged by size:
519 316 44
BglII A'GATC_T
Cuts at: 0 239 879
Size: 239 640
Bme1580I G_kGCm'C
Cuts at: 0 32 40 604 879
Size: 32 8 564 275
Fragments arranged by size:
564 275 32 8
BmgBI CAC'GTC
Cuts at: 0 408 879
Size: 408 471
BmrI ACTGGGnnnn_n'
Cuts at: 0 516 879
Size: 516 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 845 879
Size: 253 592 34
Fragments arranged by size:
592 253 34
Bpu10I CC'TnA_GC
Cuts at: 0 672 879
Size: 672 207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 879
Size: 289 590
BsaHI Gr'CG_yC
Cuts at: 0 11 879
Size: 11 868
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 774 804 843 873 879
Size: 774 30 39 30 6
Fragments arranged by size:
774 39 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 879
Size: 250 629
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 355 575 669 865 879
Size: 38 317 220 94 196 14
Fragments arranged by size:
317 220 196 94 38 14
BsiEI CG_ry'CG
Cuts at: 0 117 879
Size: 117 762
BsiHKAI G_wGCw'C
Cuts at: 0 150 430 879
Size: 150 280 449
Fragments arranged by size:
449 280 150
BsmBI CGTCTCn'nnnn_
Cuts at: 0 618 879
Size: 618 261
Bsp1286I G_dGCh'C
Cuts at: 0 32 40 150 430 604 879
Size: 32 8 110 280 174 275
Fragments arranged by size:
280 275 174 110 32 8
BspHI T'CATG_A
Cuts at: 0 641 879
Size: 641 238
BsrFI r'CCGG_y
Cuts at: 0 13 352 879
Size: 13 339 527
Fragments arranged by size:
527 339 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 382 879
Size: 382 497
BstXI CCAn_nnnn'nTGG
Cuts at: 0 438 468 879
Size: 438 30 411
Fragments arranged by size:
438 411 30
BstYI r'GATC_y
Cuts at: 0 239 879
Size: 239 640
BtgI C'CryG_G
Cuts at: 0 63 75 117 141 453 879
Size: 63 12 42 24 312 426
Fragments arranged by size:
426 312 63 42 24 12
BtsI GCAGTG_nn'
Cuts at: 0 96 686 879
Size: 96 590 193
Fragments arranged by size:
590 193 96
ClaI AT'CG_AT
Cuts at: 0 656 879
Size: 656 223
DrdI GACnn_nn'nnGTC
Cuts at: 0 817 879
Size: 817 62
EaeI y'GGCC_r
Cuts at: 0 114 354 402 440 470 879
Size: 114 240 48 38 30 409
Fragments arranged by size:
409 240 114 48 38 30
EagI C'GGCC_G
Cuts at: 0 114 879
Size: 114 765
EarI CTCTTCn'nnn_
Cuts at: 0 253 879
Size: 253 626
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 879
Size: 19 860
EcoICRI GAG'CTC
Cuts at: 0 148 879
Size: 148 731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 845 879
Size: 253 592 34
Fragments arranged by size:
592 253 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 766 879
Size: 766 113
EcoO109I rG'GnC_Cy
Cuts at: 0 28 36 37 296 879
Size: 28 8 1 259 583
Fragments arranged by size:
583 259 28 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 879
Size: 735 144
FspI TGC'GCA
Cuts at: 0 496 879
Size: 496 383
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 843 875 879
Size: 843 32 4
Fragments arranged by size:
843 32 4
MscI TGG'CCA
Cuts at: 0 404 442 472 879
Size: 404 38 30 407
Fragments arranged by size:
407 404 38 30
MspA1I CmG'CkG
Cuts at: 0 22 65 77 143 560 849 865 879
Size: 22 43 12 66 417 289 16 14
Fragments arranged by size:
417 289 66 43 22 16 14 12
NaeI GCC'GGC
Cuts at: 0 354 879
Size: 354 525
NgoMIV G'CCGG_C
Cuts at: 0 352 879
Size: 352 527
PasI CC'CwG_GG
Cuts at: 0 49 596 696 879
Size: 49 547 100 183
Fragments arranged by size:
547 183 100 49
PspOMI G'GGCC_C
Cuts at: 0 28 36 879
Size: 28 8 843
Fragments arranged by size:
843 28 8
PvuII CAG'CTG
Cuts at: 0 865 879
Size: 865 14
SacI G_AGCT'C
Cuts at: 0 150 879
Size: 150 729
SacII CC_GC'GG
Cuts at: 0 66 78 144 879
Size: 66 12 66 735
Fragments arranged by size:
735 66 66 12
SexAI A'CCwGG_T
Cuts at: 0 364 879
Size: 364 515
SfcI C'TryA_G
Cuts at: 0 741 879
Size: 741 138
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 44 879
Size: 44 835
SgrAI Cr'CCGG_yG
Cuts at: 0 13 879
Size: 13 866
SmaI CCC'GGG
Cuts at: 0 33 679 879
Size: 33 646 200
Fragments arranged by size:
646 200 33
SmlI C'TyrA_G
Cuts at: 0 304 879
Size: 304 575
StyI C'CwwG_G
Cuts at: 0 431 710 879
Size: 431 279 169
Fragments arranged by size:
431 279 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 400 406 609 879
Size: 400 6 203 270
Fragments arranged by size:
400 270 203 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 51 83 160 192 774 806 879
Size: 51 32 77 32 582 32 73
Fragments arranged by size:
582 77 73 51 32 32 32
XmaI C'CCGG_G
Cuts at: 0 31 677 879
Size: 31 646 202
Fragments arranged by size:
646 202 31
Enzymes that do cut:
AlfI ApaI AvaI BanII BbvCI BcgI BglI BglII
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaHI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrFI
BstAPI BstXI BstYI BtgI BtsI ClaI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI Hin4I MscI MspA1I NaeI NgoMIV PasI PspOMI
PvuII SacI SacII SexAI SfcI SfiI SgrAI SmaI
SmlI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BciVI BclI BfrBI BlpI BmtI BplI BsaI
BsaAI BsaBI BsaWI BseYI BsiWI BsmI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI CspCI DraI DraIII EcoRI EcoRV FseI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SalI SanDI SapI SbfI ScaI SfoI
SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XhoI XmnI ZraI