(Linear) (Six-base) MAPSORT of: KIBB3449.seq  Check: 9759  from: 1  to: 597



KIBB3449.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AleI CACnn'nnGTG

Cuts at:      0     148     597 
   Size:        148     449
 
ApoI r'AATT_y

Cuts at:      0     193     597 
   Size:        193     404
 
BanI G'GyrC_C

Cuts at:      0      26     597 
   Size:         26     571
 
BglI GCCn_nnn'nGGC

Cuts at:      0     524     597 
   Size:        524      73
 
Bme1580I G_kGCm'C

Cuts at:      0      29     157     520     597 
   Size:         29     128     363      77

  Fragments arranged by size: 

                 363     128      77      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     298     597 
   Size:        298     299
 
Bpu10I CC'TnA_GC

Cuts at:      0     338     597 
   Size:        338     259
 
BseYI C'CCAG_C

Cuts at:      0     519     597 
   Size:        519      78
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     157     520     597 
   Size:         29     128     363      77

  Fragments arranged by size: 

                 363     128      77      29
 
BsrBI CCG'CTC

Cuts at:      0     452     597 
   Size:        452     145
 
BsrDI GCAATG_nn'

Cuts at:      0     167     332     597 
   Size:        167     165     265

  Fragments arranged by size: 

                 265     167     165
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     220     527     597 
   Size:        220     307      70

  Fragments arranged by size: 

                 307     220      70
 
BtgI C'CryG_G

Cuts at:      0     158     597 
   Size:        158     439
 
BtsI GCAGTG_nn'

Cuts at:      0     546     597 
   Size:        546      51
 
DraI TTT'AAA

Cuts at:      0      78     597 
   Size:         78     519
 
EaeI y'GGCC_r

Cuts at:      0     447     597 
   Size:        447     150
 
EcoO109I rG'GnC_Cy

Cuts at:      0     292     384     597 
   Size:        292      92     213

  Fragments arranged by size: 

                 292     213      92
 
EcoRV GAT'ATC

Cuts at:      0      84     597 
   Size:         84     513
 
MslI CAynn'nnrTG

Cuts at:      0     148     597 
   Size:        148     449
 
MspA1I CmG'CkG

Cuts at:      0      55     523     597 
   Size:         55     468      74

  Fragments arranged by size: 

                 468      74      55
 
PpuMI rG'GwC_Cy

Cuts at:      0     292     597 
   Size:        292     305
 
PvuII CAG'CTG

Cuts at:      0      55     523     597 
   Size:         55     468      74

  Fragments arranged by size: 

                 468      74      55
 
SanDI GG'GwC_CC

Cuts at:      0     292     597 
   Size:        292     305
 
SpeI A'CTAG_T

Cuts at:      0     474     597 
   Size:        474     123
 
SspI AAT'ATT

Cuts at:      0     580     597 
   Size:        580      17
 
StuI AGG'CCT

Cuts at:      0     484     597 
   Size:        484     113
 

 Enzymes that do cut:

     AleI     ApoI     BanI     BglI Bme1580I     BmrI   Bpu10I    BseYI
 Bsp1286I    BsrBI    BsrDI    BstXI     BtgI     BtsI     DraI     EaeI
 EcoO109I    EcoRV     MslI   MspA1I    PpuMI    PvuII    SanDI     SpeI
     SspI     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI