(Linear) (Six-base) MAPSORT of: KIBB3448.seq  Check: 919  from: 1  to: 888



KIBB3448.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     436     888 
   Size:        436     452
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     888 
   Size:        508     380
 
ApoI r'AATT_y

Cuts at:      0      55     565     888 
   Size:         55     510     323

  Fragments arranged by size: 

                 510     323      55
 
BanI G'GyrC_C

Cuts at:      0     418     481     888 
   Size:        418      63     407

  Fragments arranged by size: 

                 418     407      63
 
BanII G_rGCy'C

Cuts at:      0     867     888 
   Size:        867      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      69     888 
   Size:         69     819
 
BglII A'GATC_T

Cuts at:      0     350     888 
   Size:        350     538
 
Bme1580I G_kGCm'C

Cuts at:      0     486     888 
   Size:        486     402
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     680     888 
   Size:        680     208
 
Bpu10I CC'TnA_GC

Cuts at:      0     866     888 
   Size:        866      22
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     697     727     888 
   Size:        697      30     161

  Fragments arranged by size: 

                 697     161      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     721     888 
   Size:        721     167
 
BsiHKAI G_wGCw'C

Cuts at:      0     867     888 
   Size:        867      21
 
Bsp1286I G_dGCh'C

Cuts at:      0     486     867     888 
   Size:        486     381      21

  Fragments arranged by size: 

                 486     381      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     436     888 
   Size:        436     452
 
BsrBI CCG'CTC

Cuts at:      0     873     888 
   Size:        873      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     235     448     888 
   Size:        235     213     440

  Fragments arranged by size: 

                 440     235     213
 
BstYI r'GATC_y

Cuts at:      0     350     888 
   Size:        350     538
 
EarI CTCTTCn'nnn_

Cuts at:      0     781     888 
   Size:        781     107
 
EcoICRI GAG'CTC

Cuts at:      0     865     888 
   Size:        865      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     680     888 
   Size:        508     172     208

  Fragments arranged by size: 

                 508     208     172
 
EcoRV GAT'ATC

Cuts at:      0     177     888 
   Size:        177     711
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      51     870     888 
   Size:         51     819      18

  Fragments arranged by size: 

                 819      51      18
 
FspI TGC'GCA

Cuts at:      0     228     888 
   Size:        228     660
 
HindIII A'AGCT_T

Cuts at:      0     852     888 
   Size:        852      36
 
NspI r_CATG'y

Cuts at:      0     624     888 
   Size:        624     264
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     256     888 
   Size:        256     632
 
PstI C_TGCA'G

Cuts at:      0     291     888 
   Size:        291     597
 
SacI G_AGCT'C

Cuts at:      0     867     888 
   Size:        867      21
 
SfcI C'TryA_G

Cuts at:      0     287     888 
   Size:        287     601
 
StuI AGG'CCT

Cuts at:      0      31     446     888 
   Size:         31     415     442

  Fragments arranged by size: 

                 442     415      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     153     888 
   Size:        153     735
 
TatI w'GTAC_w

Cuts at:      0     298     579     888 
   Size:        298     281     309

  Fragments arranged by size: 

                 309     298     281
 
XmnI GAAnn'nnTTC

Cuts at:      0     664     888 
   Size:        664     224
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     BanI    BanII     BbsI    BglII Bme1580I
     BpmI   Bpu10I    BsaXI    BseRI  BsiHKAI Bsp1286I    BspMI    BsrBI
   BstAPI    BstYI     EarI  EcoICRI  Eco57MI    EcoRV     FalI     FspI
  HindIII     NspI    PflMI     PstI     SacI     SfcI     StuI    TaqII
     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRI
     FseI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI