Restriction map for
pF1KB3446
(Linear) (Six-base) MAPSORT of: KIBB3446.seq Check: 7357 from: 1 to: 885
KIBB3446.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 641 767 812 885
Size: 283 358 126 45 73
Fragments arranged by size:
358 283 126 73 45
AfeI AGC'GCT
Cuts at: 0 804 885
Size: 804 81
AflII C'TTAA_G
Cuts at: 0 46 885
Size: 46 839
AleI CACnn'nnGTG
Cuts at: 0 74 885
Size: 74 811
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 665 885
Size: 665 220
ApoI r'AATT_y
Cuts at: 0 676 885
Size: 676 209
AvaI C'yCGr_G
Cuts at: 0 536 885
Size: 536 349
BanI G'GyrC_C
Cuts at: 0 79 559 759 885
Size: 79 480 200 126
Fragments arranged by size:
480 200 126 79
BanII G_rGCy'C
Cuts at: 0 12 504 885
Size: 12 492 381
Fragments arranged by size:
492 381 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 800 827 885
Size: 800 27 58
Fragments arranged by size:
800 58 27
BlpI GC'TnA_GC
Cuts at: 0 806 885
Size: 806 79
Bme1580I G_kGCm'C
Cuts at: 0 764 885
Size: 764 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 302 819 885
Size: 219 83 517 66
Fragments arranged by size:
517 219 83 66
Bpu10I CC'TnA_GC
Cuts at: 0 845 885
Size: 845 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 885
Size: 136 749
BsaWI w'CCGG_w
Cuts at: 0 4 885
Size: 4 881
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 438 468 885
Size: 438 30 417
Fragments arranged by size:
438 417 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 446 885
Size: 49 397 439
Fragments arranged by size:
439 397 49
BsiHKAI G_wGCw'C
Cuts at: 0 504 885
Size: 504 381
Bsp1286I G_dGCh'C
Cuts at: 0 12 504 764 885
Size: 12 492 260 121
Fragments arranged by size:
492 260 121 12
BspEI T'CCGG_A
Cuts at: 0 4 885
Size: 4 881
BsrBI CCG'CTC
Cuts at: 0 703 885
Size: 703 182
BsrDI GCAATG_nn'
Cuts at: 0 431 473 885
Size: 431 42 412
Fragments arranged by size:
431 412 42
BsrGI T'GTAC_A
Cuts at: 0 753 885
Size: 753 132
BssSI C'ACGA_G
Cuts at: 0 185 885
Size: 185 700
BstAPI GCAn_nnn'nTGC
Cuts at: 0 187 196 885
Size: 187 9 689
Fragments arranged by size:
689 187 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 76 885
Size: 76 809
BstYI r'GATC_y
Cuts at: 0 332 615 885
Size: 332 283 270
Fragments arranged by size:
332 283 270
Bsu36I CC'TnA_GG
Cuts at: 0 728 885
Size: 728 157
BtgI C'CryG_G
Cuts at: 0 355 580 815 885
Size: 355 225 235 70
Fragments arranged by size:
355 235 225 70
BtsI GCAGTG_nn'
Cuts at: 0 668 713 839 885
Size: 668 45 126 46
Fragments arranged by size:
668 126 46 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 276 311 885
Size: 276 35 574
Fragments arranged by size:
574 276 35
DraIII CAC_nnn'GTG
Cuts at: 0 510 885
Size: 510 375
EaeI y'GGCC_r
Cuts at: 0 246 300 885
Size: 246 54 585
Fragments arranged by size:
585 246 54
EarI CTCTTCn'nnn_
Cuts at: 0 617 632 885
Size: 617 15 253
Fragments arranged by size:
617 253 15
EcoICRI GAG'CTC
Cuts at: 0 502 885
Size: 502 383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 283 302 641 767 812 819 885
Size: 219 64 19 339 126 45 7 66
Fragments arranged by size:
339 219 126 66 64 45 19 7
EcoO109I rG'GnC_Cy
Cuts at: 0 158 885
Size: 158 727
FspI TGC'GCA
Cuts at: 0 550 885
Size: 550 335
HaeII r_GCGC'y
Cuts at: 0 806 885
Size: 806 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 519 551 885
Size: 519 32 334
Fragments arranged by size:
519 334 32
HincII GTy'rAC
Cuts at: 0 420 885
Size: 420 465
HindIII A'AGCT_T
Cuts at: 0 169 885
Size: 169 716
HpaI GTT'AAC
Cuts at: 0 420 885
Size: 420 465
MfeI C'AATT_G
Cuts at: 0 191 293 885
Size: 191 102 592
Fragments arranged by size:
592 191 102
MscI TGG'CCA
Cuts at: 0 248 885
Size: 248 637
MslI CAynn'nnrTG
Cuts at: 0 74 885
Size: 74 811
NcoI C'CATG_G
Cuts at: 0 580 885
Size: 580 305
NspI r_CATG'y
Cuts at: 0 555 885
Size: 555 330
PasI CC'CwG_GG
Cuts at: 0 380 885
Size: 380 505
PflMI CCAn_nnn'nTGG
Cuts at: 0 298 857 878 885
Size: 298 559 21 7
Fragments arranged by size:
559 298 21 7
PfoI T'CCnGG_A
Cuts at: 0 235 312 347 885
Size: 235 77 35 538
Fragments arranged by size:
538 235 77 35
PpuMI rG'GwC_Cy
Cuts at: 0 158 885
Size: 158 727
PstI C_TGCA'G
Cuts at: 0 834 885
Size: 834 51
SacI G_AGCT'C
Cuts at: 0 504 885
Size: 504 381
SapI GCTCTTCn'nnn_
Cuts at: 0 632 885
Size: 632 253
SfcI C'TryA_G
Cuts at: 0 609 830 885
Size: 609 221 55
Fragments arranged by size:
609 221 55
SmlI C'TyrA_G
Cuts at: 0 46 151 885
Size: 46 105 734
Fragments arranged by size:
734 105 46
SphI G_CATG'C
Cuts at: 0 555 885
Size: 555 330
StyI C'CwwG_G
Cuts at: 0 580 668 885
Size: 580 88 217
Fragments arranged by size:
580 217 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 593 885
Size: 593 292
TatI w'GTAC_w
Cuts at: 0 107 602 753 885
Size: 107 495 151 132
Fragments arranged by size:
495 151 132 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 438 470 885
Size: 438 32 415
Fragments arranged by size:
438 415 32
Enzymes that do cut:
AcuI AfeI AflII AleI AlfI ApoI AvaI BanI
BanII BbsI BlpI Bme1580I BpmI Bpu10I BpuEI BsaWI
BsaXI BseRI BsiHKAI Bsp1286I BspEI BsrBI BsrDI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FspI HaeII
Hin4I HincII HindIII HpaI MfeI MscI MslI NcoI
NspI PasI PflMI PfoI PpuMI PstI SacI SapI
SfcI SmlI SphI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaHI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BspMI BsrFI BssHII BstBI BstEII BstZ17I BtgZI
ClaI DraI DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspAI KasI KpnI MluI MmeI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI