(Linear) (Six-base) MAPSORT of: KIBB3445.seq  Check: 9087  from: 1  to: 606



KIBB3445.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     227     273     606 
   Size:        227      46     333

  Fragments arranged by size: 

                 333     227      46
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     258     606 
   Size:        258     348
 
BamHI G'GATC_C

Cuts at:      0     213     606 
   Size:        213     393
 
BanII G_rGCy'C

Cuts at:      0     322     440     606 
   Size:        322     118     166

  Fragments arranged by size: 

                 322     166     118
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     411     606 
   Size:        411     195
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586     606 
   Size:        586      20
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     214     606 
   Size:        214     392
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      19      49     606 
   Size:         19      30     557

  Fragments arranged by size: 

                 557      30      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     606 
   Size:        356     250
 
Bsp1286I G_dGCh'C

Cuts at:      0     322     440     606 
   Size:        322     118     166

  Fragments arranged by size: 

                 322     166     118
 
BsrBI CCG'CTC

Cuts at:      0      30     134     606 
   Size:         30     104     472

  Fragments arranged by size: 

                 472     104      30
 
BsrGI T'GTAC_A

Cuts at:      0     518     606 
   Size:        518      88
 
BstEII G'GTnAC_C

Cuts at:      0     297     606 
   Size:        297     309
 
BstYI r'GATC_y

Cuts at:      0     213     606 
   Size:        213     393
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     157     606 
   Size:        157     449
 
EaeI y'GGCC_r

Cuts at:      0     347     359     606 
   Size:        347      12     247

  Fragments arranged by size: 

                 347     247      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586     606 
   Size:        586      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     451     606 
   Size:        451     155
 
EcoO109I rG'GnC_Cy

Cuts at:      0     461     606 
   Size:        461     145
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       3      35     213     245     259     291     481     513 
   Size:          3      32     178      32      14      32     190      32

Cuts at:    513     606 
   Size:         93

  Fragments arranged by size: 

                 190     178      93      32      32      32      32      14
                   3
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     484     606 
   Size:         67     417     122

  Fragments arranged by size: 

                 417     122      67
 
MscI TGG'CCA

Cuts at:      0     361     606 
   Size:        361     245
 
MslI CAynn'nnrTG

Cuts at:      0     230     606 
   Size:        230     376
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      62      94     606 
   Size:         62      32     512

  Fragments arranged by size: 

                 512      62      32
 
PstI C_TGCA'G

Cuts at:      0     445     606 
   Size:        445     161
 
SexAI A'CCwGG_T

Cuts at:      0     301     606 
   Size:        301     305
 
SfcI C'TryA_G

Cuts at:      0     441     606 
   Size:        441     165
 
StyI C'CwwG_G

Cuts at:      0     544     606 
   Size:        544      62
 
TatI w'GTAC_w

Cuts at:      0     518     606 
   Size:        518      88
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     542     606 
   Size:        542      64
 

 Enzymes that do cut:

     AhdI     AlfI    BamHI    BanII    BciVI     BpmI     BsaI    BsaXI
     BsgI Bsp1286I    BsrBI    BsrGI   BstEII    BstYI     DrdI     EaeI
  Eco57MI    EcoNI EcoO109I    Hin4I     MmeI     MscI     MslI     PpiI
     PstI    SexAI     SfcI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI