(Linear) (Six-base) MAPSORT of: KIBB3444.seq  Check: 834  from: 1  to: 420



KIBB3444.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

ApoI r'AATT_y

Cuts at:      0      37     420 
   Size:         37     383
 
BanII G_rGCy'C

Cuts at:      0     215     420 
   Size:        215     205
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      49     420 
   Size:         49     371
 
BmgBI CAC'GTC

Cuts at:      0      16     420 
   Size:         16     404
 
BsaWI w'CCGG_w

Cuts at:      0      41      60     420 
   Size:         41      19     360

  Fragments arranged by size: 

                 360      41      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     299     329     420 
   Size:        299      30      91

  Fragments arranged by size: 

                 299      91      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     420 
   Size:        395      25
 
BsiHKAI G_wGCw'C

Cuts at:      0     215     420 
   Size:        215     205
 
Bsp1286I G_dGCh'C

Cuts at:      0     215     420 
   Size:        215     205
 
BspEI T'CCGG_A

Cuts at:      0      41     420 
   Size:         41     379
 
BsrDI GCAATG_nn'

Cuts at:      0     413     420 
   Size:        413       7
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     403     420 
   Size:        403      17
 
BtsI GCAGTG_nn'

Cuts at:      0     242     420 
   Size:        242     178
 
EaeI y'GGCC_r

Cuts at:      0     185     420 
   Size:        185     235
 
EcoICRI GAG'CTC

Cuts at:      0     213     420 
   Size:        213     207
 
EcoO109I rG'GnC_Cy

Cuts at:      0      23     420 
   Size:         23     397
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      59      91     420 
   Size:         59      32     329

  Fragments arranged by size: 

                 329      59      32
 
MfeI C'AATT_G

Cuts at:      0      78     420 
   Size:         78     342
 
MscI TGG'CCA

Cuts at:      0     187     420 
   Size:        187     233
 
MslI CAynn'nnrTG

Cuts at:      0     121     181     420 
   Size:        121      60     239

  Fragments arranged by size: 

                 239     121      60
 
MspA1I CmG'CkG

Cuts at:      0     160     420 
   Size:        160     260
 
PpuMI rG'GwC_Cy

Cuts at:      0      23     420 
   Size:         23     397
 
SacI G_AGCT'C

Cuts at:      0     215     420 
   Size:        215     205
 
StyI C'CwwG_G

Cuts at:      0      50     420 
   Size:         50     370
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     160     420 
   Size:        160     260
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     299     331     420 
   Size:        299      32      89

  Fragments arranged by size: 

                 299      89      32
 

 Enzymes that do cut:

     ApoI    BanII     BbsI    BmgBI    BsaWI    BsaXI     BsgI  BsiHKAI
 Bsp1286I    BspEI    BsrDI   BstAPI     BtsI     EaeI  EcoICRI EcoO109I
    Hin4I     MfeI     MscI     MslI   MspA1I    PpuMI     SacI     StyI
    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI