(Linear) (Six-base) MAPSORT of: KIBB3443.seq  Check: 5265  from: 1  to: 744



KIBB3443.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

Acc65I G'GTAC_C

Cuts at:      0     428     744 
   Size:        428     316
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     460     744 
   Size:        103     357     284

  Fragments arranged by size: 

                 357     284     103
 
AflII C'TTAA_G

Cuts at:      0      52     744 
   Size:         52     692
 
ApoI r'AATT_y

Cuts at:      0      69     203     744 
   Size:         69     134     541

  Fragments arranged by size: 

                 541     134      69
 
BanI G'GyrC_C

Cuts at:      0     428     744 
   Size:        428     316
 
BanII G_rGCy'C

Cuts at:      0     307     744 
   Size:        307     437
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     632     744 
   Size:        632     112
 
BbvCI CC'TCA_GC

Cuts at:      0     397     744 
   Size:        397     347
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     316     350     375     409     744 
   Size:        316      34      25      34     335

  Fragments arranged by size: 

                 335     316      34      34      25
 
BglII A'GATC_T

Cuts at:      0     578     744 
   Size:        578     166
 
Bpu10I CC'TnA_GC

Cuts at:      0     397     744 
   Size:        397     347
 
Bsp1286I G_dGCh'C

Cuts at:      0     307     744 
   Size:        307     437
 
BspHI T'CATG_A

Cuts at:      0     573     744 
   Size:        573     171
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     258     744 
   Size:        258     486
 
BsrGI T'GTAC_A

Cuts at:      0     191     744 
   Size:        191     553
 
BssSI C'ACGA_G

Cuts at:      0     647     744 
   Size:        647      97
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     554     744 
   Size:        554     190
 
BstBI TT'CG_AA

Cuts at:      0     126     207     315     744 
   Size:        126      81     108     429

  Fragments arranged by size: 

                 429     126     108      81
 
BstYI r'GATC_y

Cuts at:      0     578     744 
   Size:        578     166
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     660     695     744 
   Size:        660      35      49

  Fragments arranged by size: 

                 660      49      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     652     744 
   Size:        652      92
 
EarI CTCTTCn'nnn_

Cuts at:      0       8     744 
   Size:          8     736
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     460     744 
   Size:        103     357     284

  Fragments arranged by size: 

                 357     284     103
 
HaeII r_GCGC'y

Cuts at:      0      96     744 
   Size:         96     648
 
HindIII A'AGCT_T

Cuts at:      0     142     744 
   Size:        142     602
 
KpnI G_GTAC'C

Cuts at:      0     432     744 
   Size:        432     312
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     570     744 
   Size:        570     174
 
MslI CAynn'nnrTG

Cuts at:      0     556     744 
   Size:        556     188
 
NspI r_CATG'y

Cuts at:      0     396     744 
   Size:        396     348
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     652     744 
   Size:        652      92
 
PstI C_TGCA'G

Cuts at:      0     255     744 
   Size:        255     489
 
SfcI C'TryA_G

Cuts at:      0     251     744 
   Size:        251     493
 
SmlI C'TyrA_G

Cuts at:      0      52     744 
   Size:         52     692
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     688     744 
   Size:        688      56
 
TatI w'GTAC_w

Cuts at:      0     191     744 
   Size:        191     553
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     350     382     744 
   Size:        350      32     362

  Fragments arranged by size: 

                 362     350      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     419     744 
   Size:        419     325
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII     ApoI     BanI    BanII     BbsI    BbvCI
     BcgI    BglII   Bpu10I Bsp1286I    BspHI    BspMI    BsrGI    BssSI
   BstAPI    BstBI    BstYI    CspCI   DraIII     EarI  Eco57MI    HaeII
  HindIII     KpnI     MmeI     MslI     NspI    PflMI     PstI     SfcI
     SmlI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KasI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI