(Linear) (Six-base) MAPSORT of: KIBB3437.seq  Check: 3438  from: 1  to: 657



KIBB3437.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     657 
   Size:         46     611
 
AlwNI CAG_nnn'CTG

Cuts at:      0     327     657 
   Size:        327     330
 
BanII G_rGCy'C

Cuts at:      0     181     220     657 
   Size:        181      39     437

  Fragments arranged by size: 

                 437     181      39
 
BbvCI CC'TCA_GC

Cuts at:      0     284     657 
   Size:        284     373
 
Bpu10I CC'TnA_GC

Cuts at:      0     284     344     657 
   Size:        284      60     313

  Fragments arranged by size: 

                 313     284      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     127     157     657 
   Size:        127      30     500

  Fragments arranged by size: 

                 500     127      30
 
BseYI C'CCAG_C

Cuts at:      0     153     657 
   Size:        153     504
 
BsiHKAI G_wGCw'C

Cuts at:      0     128     657 
   Size:        128     529
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     181     220     657 
   Size:        128      53      39     437

  Fragments arranged by size: 

                 437     128      53      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     107     657 
   Size:        107     550
 
BsrDI GCAATG_nn'

Cuts at:      0     165     657 
   Size:        165     492
 
BssSI C'ACGA_G

Cuts at:      0     447     657 
   Size:        447     210
 
BstEII G'GTnAC_C

Cuts at:      0     288     657 
   Size:        288     369
 
BstYI r'GATC_y

Cuts at:      0      62      92     657 
   Size:         62      30     565

  Fragments arranged by size: 

                 565      62      30
 
BtsI GCAGTG_nn'

Cuts at:      0     433     657 
   Size:        433     224
 
EaeI y'GGCC_r

Cuts at:      0     296     657 
   Size:        296     361
 
EarI CTCTTCn'nnn_

Cuts at:      0     133     657 
   Size:        133     524
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     657 
   Size:         46     611
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     283     657 
   Size:        283     374
 
MscI TGG'CCA

Cuts at:      0     298     657 
   Size:        298     359
 
PstI C_TGCA'G

Cuts at:      0     428     657 
   Size:        428     229
 
SapI GCTCTTCn'nnn_

Cuts at:      0     133     657 
   Size:        133     524
 
SfcI C'TryA_G

Cuts at:      0     424     657 
   Size:        424     233
 
StyI C'CwwG_G

Cuts at:      0     293     392     657 
   Size:        293      99     265

  Fragments arranged by size: 

                 293     265      99
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     370     657 
   Size:        370     287
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     220     252     657 
   Size:        220      32     405

  Fragments arranged by size: 

                 405     220      32
 

 Enzymes that do cut:

     AcuI    AlwNI    BanII    BbvCI   Bpu10I    BsaXI    BseYI  BsiHKAI
 Bsp1286I    BspMI    BsrDI    BssSI   BstEII    BstYI     BtsI     EaeI
     EarI  Eco57MI    EcoNI     MscI     PstI     SapI     SfcI     StyI
    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI