Restriction map for
pF1KB3435
(Linear) (Six-base) MAPSORT of: KIBB3435.seq Check: 5628 from: 1 to: 1218
KIBB3435.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
Acc65I G'GTAC_C
Cuts at: 0 469 1218
Size: 469 749
AleI CACnn'nnGTG
Cuts at: 0 415 1218
Size: 415 803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 433 1027 1218
Size: 433 594 191
Fragments arranged by size:
594 433 191
AlwNI CAG_nnn'CTG
Cuts at: 0 438 1218
Size: 438 780
AvaI C'yCGr_G
Cuts at: 0 326 881 1218
Size: 326 555 337
Fragments arranged by size:
555 337 326
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 384 417 1218
Size: 384 33 801
Fragments arranged by size:
801 384 33
BanI G'GyrC_C
Cuts at: 0 382 409 469 1218
Size: 382 27 60 749
Fragments arranged by size:
749 382 60 27
BanII G_rGCy'C
Cuts at: 0 56 1218
Size: 56 1162
BbeI G_GCGC'C
Cuts at: 0 386 1218
Size: 386 832
BbsI GAAGACnn'nnnn_
Cuts at: 0 1142 1218
Size: 1142 76
BbvCI CC'TCA_GC
Cuts at: 0 425 1218
Size: 425 793
BciVI GTATCCnnnnn_n'
Cuts at: 0 745 906 1218
Size: 745 161 312
Fragments arranged by size:
745 312 161
BfrBI ATG'CAT
Cuts at: 0 876 906 1218
Size: 876 30 312
Fragments arranged by size:
876 312 30
BglI GCCn_nnn'nGGC
Cuts at: 0 304 1218
Size: 304 914
BglII A'GATC_T
Cuts at: 0 288 584 1218
Size: 288 296 634
Fragments arranged by size:
634 296 288
BlpI GC'TnA_GC
Cuts at: 0 338 1218
Size: 338 880
Bme1580I G_kGCm'C
Cuts at: 0 412 1218
Size: 412 806
BmgBI CAC'GTC
Cuts at: 0 936 1218
Size: 936 282
BmrI ACTGGGnnnn_n'
Cuts at: 0 248 1218
Size: 248 970
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 180 1218
Size: 180 1038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1218
Size: 748 470
Bpu10I CC'TnA_GC
Cuts at: 0 425 1218
Size: 425 793
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 682 1218
Size: 206 476 536
Fragments arranged by size:
536 476 206
BsaI GGTCTCn'nnnn_
Cuts at: 0 1156 1218
Size: 1156 62
BsaHI Gr'CG_yC
Cuts at: 0 62 383 1218
Size: 62 321 835
Fragments arranged by size:
835 321 62
BsaWI w'CCGG_w
Cuts at: 0 500 1218
Size: 500 718
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1162 1192 1218
Size: 1162 30 26
Fragments arranged by size:
1162 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 781 1218
Size: 781 437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 1033 1218
Size: 451 582 185
Fragments arranged by size:
582 451 185
BsiEI CG_ry'CG
Cuts at: 0 1083 1218
Size: 1083 135
BsmBI CGTCTCn'nnnn_
Cuts at: 0 926 1218
Size: 926 292
Bsp1286I G_dGCh'C
Cuts at: 0 56 412 1218
Size: 56 356 806
Fragments arranged by size:
806 356 56
BspHI T'CATG_A
Cuts at: 0 680 1218
Size: 680 538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 998 1008 1218
Size: 998 10 210
Fragments arranged by size:
998 210 10
BstAPI GCAn_nnn'nTGC
Cuts at: 0 438 1218
Size: 438 780
BstEII G'GTnAC_C
Cuts at: 0 666 1218
Size: 666 552
BstYI r'GATC_y
Cuts at: 0 288 584 1218
Size: 288 296 634
Fragments arranged by size:
634 296 288
BtgI C'CryG_G
Cuts at: 0 132 567 1218
Size: 132 435 651
Fragments arranged by size:
651 435 132
ClaI AT'CG_AT
Cuts at: 0 265 1218
Size: 265 953
DraIII CAC_nnn'GTG
Cuts at: 0 790 1218
Size: 790 428
EaeI y'GGCC_r
Cuts at: 0 248 477 995 1098 1218
Size: 248 229 518 103 120
Fragments arranged by size:
518 248 229 120 103
EarI CTCTTCn'nnn_
Cuts at: 0 710 1154 1218
Size: 710 444 64
Fragments arranged by size:
710 444 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 727 888 1218
Size: 727 161 330
Fragments arranged by size:
727 330 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1218
Size: 748 470
EcoO109I rG'GnC_Cy
Cuts at: 0 687 1218
Size: 687 531
HaeII r_GCGC'y
Cuts at: 0 386 1218
Size: 386 832
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 435 467 864 896 1218
Size: 7 32 396 32 397 32 322
Fragments arranged by size:
397 396 322 32 32 32 7
HincII GTy'rAC
Cuts at: 0 93 722 1218
Size: 93 629 496
Fragments arranged by size:
629 496 93
KasI G'GCGC_C
Cuts at: 0 382 1218
Size: 382 836
KpnI G_GTAC'C
Cuts at: 0 473 1218
Size: 473 745
MscI TGG'CCA
Cuts at: 0 997 1218
Size: 997 221
MslI CAynn'nnrTG
Cuts at: 0 415 463 529 754 1009 1218
Size: 415 48 66 225 255 209
Fragments arranged by size:
415 255 225 209 66 48
NarI GG'CG_CC
Cuts at: 0 383 1218
Size: 383 835
NsiI A_TGCA'T
Cuts at: 0 878 908 1218
Size: 878 30 310
Fragments arranged by size:
878 310 30
NspI r_CATG'y
Cuts at: 0 880 1218
Size: 880 338
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 1218
Size: 309 909
PpuMI rG'GwC_Cy
Cuts at: 0 687 1218
Size: 687 531
PstI C_TGCA'G
Cuts at: 0 275 443 1218
Size: 275 168 775
Fragments arranged by size:
775 275 168
SanDI GG'GwC_CC
Cuts at: 0 687 1218
Size: 687 531
SfcI C'TryA_G
Cuts at: 0 271 439 1218
Size: 271 168 779
Fragments arranged by size:
779 271 168
SfoI GGC'GCC
Cuts at: 0 384 1218
Size: 384 834
SmlI C'TyrA_G
Cuts at: 0 221 326 661 881 1218
Size: 221 105 335 220 337
Fragments arranged by size:
337 335 221 220 105
SphI G_CATG'C
Cuts at: 0 880 1218
Size: 880 338
StuI AGG'CCT
Cuts at: 0 307 1218
Size: 307 911
StyI C'CwwG_G
Cuts at: 0 308 1218
Size: 308 910
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 336 634 832 1069 1218
Size: 316 20 298 198 237 149
Fragments arranged by size:
316 298 237 198 149 20
TatI w'GTAC_w
Cuts at: 0 537 1218
Size: 537 681
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 235 306 330 990 1218
Size: 235 71 24 660 228
Fragments arranged by size:
660 235 228 71 24
XhoI C'TCGA_G
Cuts at: 0 326 881 1218
Size: 326 555 337
Fragments arranged by size:
555 337 326
Enzymes that do cut:
Acc65I AleI AlfI AlwNI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsmBI Bsp1286I
BspHI BspMI BstAPI BstEII BstYI BtgI ClaI DraIII
EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII
KasI KpnI MscI MslI NarI NsiI NspI PflMI
PpuMI PstI SanDI SfcI SfoI SmlI SphI StuI
StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BcgI BclI BmtI BsaAI BsaBI
BseYI BsiHKAI BsiWI BsmI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI
BtsI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI
MluI MmeI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TstI Tth111I XbaI XmaI XmnI ZraI