(Linear) (Six-base) MAPSORT of: KIBB3431.seq  Check: 9670  from: 1  to: 675



KIBB3431.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     638     675 
   Size:        638      37
 
BanII G_rGCy'C

Cuts at:      0     520     675 
   Size:        520     155
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     260     410     675 
   Size:        260     150     265

  Fragments arranged by size: 

                 265     260     150
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     535     675 
   Size:        535     140
 
BglI GCCn_nnn'nGGC

Cuts at:      0      29     675 
   Size:         29     646
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     628     675 
   Size:         19     609      47

  Fragments arranged by size: 

                 609      47      19
 
Bpu10I CC'TnA_GC

Cuts at:      0     352     675 
   Size:        352     323
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       5     675 
   Size:          5     670
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     340     490     625     675 
   Size:        340     150     135      50

  Fragments arranged by size: 

                 340     150     135      50
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     661     675 
   Size:        386     275      14

  Fragments arranged by size: 

                 386     275      14
 
BsiHKAI G_wGCw'C

Cuts at:      0      44     675 
   Size:         44     631
 
BsmI GAATG_Cn'

Cuts at:      0     491     675 
   Size:        491     184
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     520     675 
   Size:         44     476     155

  Fragments arranged by size: 

                 476     155      44
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     408     675 
   Size:        408     267
 
BsrFI r'CCGG_y

Cuts at:      0      28     675 
   Size:         28     647
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     409     675 
   Size:        409     266
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     654     675 
   Size:        654      21
 
BstYI r'GATC_y

Cuts at:      0     313     675 
   Size:        313     362
 
BtgI C'CryG_G

Cuts at:      0     647     675 
   Size:        647      28
 
BtsI GCAGTG_nn'

Cuts at:      0     620     675 
   Size:        620      55
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     508     675 
   Size:        508     167
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      73     675 
   Size:         73     602
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     628     675 
   Size:         19     609      47

  Fragments arranged by size: 

                 609      47      19
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     382     675 
   Size:        382     293
 
HindIII A'AGCT_T

Cuts at:      0     221     675 
   Size:        221     454
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     573     675 
   Size:        573     102
 
MslI CAynn'nnrTG

Cuts at:      0     652     675 
   Size:        652      23
 
NaeI GCC'GGC

Cuts at:      0      30     675 
   Size:         30     645
 
NcoI C'CATG_G

Cuts at:      0     647     675 
   Size:        647      28
 
NgoMIV G'CCGG_C

Cuts at:      0      28     675 
   Size:         28     647
 
PshAI GACnn'nnGTC

Cuts at:      0     146     675 
   Size:        146     529
 
SfcI C'TryA_G

Cuts at:      0     260     275     675 
   Size:        260      15     400

  Fragments arranged by size: 

                 400     260      15
 
SpeI A'CTAG_T

Cuts at:      0     528     675 
   Size:        528     147
 
StyI C'CwwG_G

Cuts at:      0     442     647     675 
   Size:        442     205      28

  Fragments arranged by size: 

                 442     205      28
 

 Enzymes that do cut:

     AlfI    BanII     BbsI    BciVI     BglI     BpmI   Bpu10I     BsaI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrFI   BstAPI
    BstXI    BstYI     BtgI     BtsI     DrdI     EciI  Eco57MI     FalI
  HindIII     MmeI     MslI     NaeI     NcoI   NgoMIV    PshAI     SfcI
     SpeI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI
     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NarI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI