(Linear) (Six-base) MAPSORT of: KIBB3429.seq  Check: 7432  from: 1  to: 576



KIBB3429.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0      29      47     576 
   Size:         29      18     529

  Fragments arranged by size: 

                 529      29      18
 
AclI AA'CG_TT

Cuts at:      0     305     576 
   Size:        305     271
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     576 
   Size:         79     497
 
ApaI G_GGCC'C

Cuts at:      0      88     576 
   Size:         88     488
 
ApoI r'AATT_y

Cuts at:      0     345     465     520     576 
   Size:        345     120      55      56

  Fragments arranged by size: 

                 345     120      56      55
 
AseI AT'TA_AT

Cuts at:      0     430     576 
   Size:        430     146
 
BanII G_rGCy'C

Cuts at:      0      88     576 
   Size:         88     488
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     576 
   Size:         10     566
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      57      91     576 
   Size:         57      34     485

  Fragments arranged by size: 

                 485      57      34
 
BclI T'GATC_A

Cuts at:      0     239     576 
   Size:        239     337
 
Bme1580I G_kGCm'C

Cuts at:      0      88     576 
   Size:         88     488
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     265     576 
   Size:        265     311
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     576 
   Size:        395     181
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     332     378     576 
   Size:        332      46     198

  Fragments arranged by size: 

                 332     198      46
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     576 
   Size:        310      30     236

  Fragments arranged by size: 

                 310     236      30
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     576 
   Size:         88     488
 
BsrGI T'GTAC_A

Cuts at:      0     228     576 
   Size:        228     348
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     322     576 
   Size:        322     254
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     576 
   Size:         79     497
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84      85     576 
   Size:         84       1     491

  Fragments arranged by size: 

                 491      84       1
 
EcoRV GAT'ATC

Cuts at:      0     513     576 
   Size:        513      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342     576 
   Size:        310      32     234

  Fragments arranged by size: 

                 310     234      32
 
HincII GTy'rAC

Cuts at:      0      30      48     171     576 
   Size:         30      18     123     405

  Fragments arranged by size: 

                 405     123      30      18
 
MslI CAynn'nnrTG

Cuts at:      0     122     576 
   Size:        122     454
 
MspA1I CmG'CkG

Cuts at:      0     472     576 
   Size:        472     104
 
PfoI T'CCnGG_A

Cuts at:      0     314     576 
   Size:        314     262
 
PspOMI G'GGCC_C

Cuts at:      0      84     576 
   Size:         84     492
 
SalI G'TCGA_C

Cuts at:      0      28      46     576 
   Size:         28      18     530

  Fragments arranged by size: 

                 530      28      18
 
SexAI A'CCwGG_T

Cuts at:      0     387     576 
   Size:        387     189
 
SmlI C'TyrA_G

Cuts at:      0     410     576 
   Size:        410     166
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     343     576 
   Size:        343     233
 
TatI w'GTAC_w

Cuts at:      0     228     576 
   Size:        228     348
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     245     277     576 
   Size:        245      32     299

  Fragments arranged by size: 

                 299     245      32
 

 Enzymes that do cut:

     AccI     AclI     AcuI     ApaI     ApoI     AseI    BanII     BbsI
     BcgI     BclI Bme1580I     BmrI    BpuEI     BsaI    BsaXI Bsp1286I
    BsrGI    BstXI  Eco57MI EcoO109I    EcoRV    Hin4I   HincII     MslI
   MspA1I     PfoI   PspOMI     SalI    SexAI     SmlI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI