Restriction map for
pF1KB3427
(Linear) (Six-base) MAPSORT of: KIBB3427.seq Check: 9106 from: 1 to: 1275
KIBB3427.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AccI GT'mk_AC
Cuts at: 0 453 1275
Size: 453 822
Acc65I G'GTAC_C
Cuts at: 0 598 1275
Size: 598 677
AclI AA'CG_TT
Cuts at: 0 246 510 906 1275
Size: 246 264 396 369
Fragments arranged by size:
396 369 264 246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1275
Size: 1097 178
AleI CACnn'nnGTG
Cuts at: 0 323 1275
Size: 323 952
AlwNI CAG_nnn'CTG
Cuts at: 0 967 1275
Size: 967 308
ApaLI G'TGCA_C
Cuts at: 0 274 784 1275
Size: 274 510 491
Fragments arranged by size:
510 491 274
ApoI r'AATT_y
Cuts at: 0 103 1275
Size: 103 1172
BanI G'GyrC_C
Cuts at: 0 598 1275
Size: 598 677
BbvCI CC'TCA_GC
Cuts at: 0 233 1275
Size: 233 1042
BciVI GTATCCnnnnn_n'
Cuts at: 0 1229 1275
Size: 1229 46
Bme1580I G_kGCm'C
Cuts at: 0 278 788 1275
Size: 278 510 487
Fragments arranged by size:
510 487 278
BmtI G_CTAG'C
Cuts at: 0 770 1275
Size: 770 505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 226 532 1196 1275
Size: 29 197 306 664 79
Fragments arranged by size:
664 306 197 79 29
Bpu10I CC'TnA_GC
Cuts at: 0 233 437 1275
Size: 233 204 838
Fragments arranged by size:
838 233 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 849 1275
Size: 808 41 426
Fragments arranged by size:
808 426 41
BsaAI yAC'GTr
Cuts at: 0 273 1275
Size: 273 1002
BsaWI w'CCGG_w
Cuts at: 0 107 1275
Size: 107 1168
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1225 1275
Size: 1225 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1213 1275
Size: 470 743 62
Fragments arranged by size:
743 470 62
BsiHKAI G_wGCw'C
Cuts at: 0 278 788 1275
Size: 278 510 487
Fragments arranged by size:
510 487 278
BsmI GAATG_Cn'
Cuts at: 0 1115 1275
Size: 1115 160
Bsp1286I G_dGCh'C
Cuts at: 0 278 788 1275
Size: 278 510 487
Fragments arranged by size:
510 487 278
BspEI T'CCGG_A
Cuts at: 0 107 1275
Size: 107 1168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 395 584 1275
Size: 395 189 691
Fragments arranged by size:
691 395 189
BsrBI CCG'CTC
Cuts at: 0 41 1275
Size: 41 1234
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1128 1275
Size: 1128 147
BstYI r'GATC_y
Cuts at: 0 531 543 1013 1275
Size: 531 12 470 262
Fragments arranged by size:
531 470 262 12
BtgI C'CryG_G
Cuts at: 0 1163 1275
Size: 1163 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 886 1275
Size: 886 389
BtsI GCAGTG_nn'
Cuts at: 0 288 1275
Size: 288 987
EaeI y'GGCC_r
Cuts at: 0 285 1022 1139 1160 1275
Size: 285 737 117 21 115
Fragments arranged by size:
737 285 117 115 21
EarI CTCTTCn'nnn_
Cuts at: 0 233 1202 1275
Size: 233 969 73
Fragments arranged by size:
969 233 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 1275
Size: 154 1121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 226 532 1097 1196 1275
Size: 29 197 306 565 99 79
Fragments arranged by size:
565 306 197 99 79 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 516 548 692 724 1275
Size: 181 32 303 32 144 32 551
Fragments arranged by size:
551 303 181 144 32 32 32
HindIII A'AGCT_T
Cuts at: 0 400 1275
Size: 400 875
KpnI G_GTAC'C
Cuts at: 0 602 1275
Size: 602 673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 1275
Size: 324 951
MscI TGG'CCA
Cuts at: 0 287 1141 1162 1275
Size: 287 854 21 113
Fragments arranged by size:
854 287 113 21
MslI CAynn'nnrTG
Cuts at: 0 323 1156 1162 1275
Size: 323 833 6 113
Fragments arranged by size:
833 323 113 6
MspA1I CmG'CkG
Cuts at: 0 123 232 492 1275
Size: 123 109 260 783
Fragments arranged by size:
783 260 123 109
NcoI C'CATG_G
Cuts at: 0 1163 1275
Size: 1163 112
NdeI CA'TA_TG
Cuts at: 0 1236 1275
Size: 1236 39
NheI G'CTAG_C
Cuts at: 0 766 1275
Size: 766 509
PfoI T'CCnGG_A
Cuts at: 0 185 1275
Size: 185 1090
PmlI CAC'GTG
Cuts at: 0 273 1275
Size: 273 1002
PshAI GACnn'nnGTC
Cuts at: 0 940 1275
Size: 940 335
PstI C_TGCA'G
Cuts at: 0 309 629 916 1067 1275
Size: 309 320 287 151 208
Fragments arranged by size:
320 309 287 208 151
PvuII CAG'CTG
Cuts at: 0 123 232 492 1275
Size: 123 109 260 783
Fragments arranged by size:
783 260 123 109
ScaI AGT'ACT
Cuts at: 0 752 1275
Size: 752 523
SfcI C'TryA_G
Cuts at: 0 305 625 912 955 1063 1275
Size: 305 320 287 43 108 212
Fragments arranged by size:
320 305 287 212 108 43
SmlI C'TyrA_G
Cuts at: 0 823 864 1275
Size: 823 41 411
Fragments arranged by size:
823 411 41
StyI C'CwwG_G
Cuts at: 0 321 1163 1275
Size: 321 842 112
Fragments arranged by size:
842 321 112
TatI w'GTAC_w
Cuts at: 0 750 1275
Size: 750 525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1073 1105 1275
Size: 1073 32 170
Fragments arranged by size:
1073 170 32
Enzymes that do cut:
AccI Acc65I AclI AcuI AleI AlwNI ApaLI ApoI
BanI BbvCI BciVI Bme1580I BmtI BpmI Bpu10I BpuEI
BsaAI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BstAPI BstYI BtgI BtgZI BtsI EaeI
EarI EciI Eco57MI Hin4I HindIII KpnI MmeI MscI
MslI MspA1I NcoI NdeI NheI PfoI PmlI PshAI
PstI PvuII ScaI SfcI SmlI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BbsI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BplI BsaI BsaBI BsaHI
BsaXI BseYI BsiEI BsiWI BsmBI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI MfeI MluI NaeI NarI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI