(Linear) (Six-base) MAPSORT of: KIBB3422.seq  Check: 7759  from: 1  to: 393



KIBB3422.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AleI CACnn'nnGTG

Cuts at:      0     191     393 
   Size:        191     202
 
AvaI C'yCGr_G

Cuts at:      0      94     393 
   Size:         94     299
 
BanII G_rGCy'C

Cuts at:      0      91     152     393 
   Size:         91      61     241

  Fragments arranged by size: 

                 241      91      61
 
BmgBI CAC'GTC

Cuts at:      0       9     393 
   Size:          9     384
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     361     393 
   Size:        361      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     393 
   Size:        224     169
 
BsiHKAI G_wGCw'C

Cuts at:      0      91     131     152     393 
   Size:         91      40      21     241

  Fragments arranged by size: 

                 241      91      40      21
 
Bsp1286I G_dGCh'C

Cuts at:      0      91     131     152     393 
   Size:         91      40      21     241

  Fragments arranged by size: 

                 241      91      40      21
 
BspHI T'CATG_A

Cuts at:      0      80     393 
   Size:         80     313
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     238     393 
   Size:        238     155
 
BtgI C'CryG_G

Cuts at:      0     143     393 
   Size:        143     250
 
BtsI GCAGTG_nn'

Cuts at:      0     198     236     393 
   Size:        198      38     157

  Fragments arranged by size: 

                 198     157      38
 
EaeI y'GGCC_r

Cuts at:      0     134     393 
   Size:        134     259
 
EarI CTCTTCn'nnn_

Cuts at:      0      79     393 
   Size:         79     314
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     393 
   Size:         19     374
 
EcoICRI GAG'CTC

Cuts at:      0      89     150     393 
   Size:         89      61     243

  Fragments arranged by size: 

                 243      89      61
 
MslI CAynn'nnrTG

Cuts at:      0     191     393 
   Size:        191     202
 
MspA1I CmG'CkG

Cuts at:      0      42     393 
   Size:         42     351
 
SacI G_AGCT'C

Cuts at:      0      91     152     393 
   Size:         91      61     241

  Fragments arranged by size: 

                 241      91      61
 
SapI GCTCTTCn'nnn_

Cuts at:      0      79     393 
   Size:         79     314
 
ScaI AGT'ACT

Cuts at:      0     212     393 
   Size:        212     181
 
SfcI C'TryA_G

Cuts at:      0     180     393 
   Size:        180     213
 
StyI C'CwwG_G

Cuts at:      0     137     393 
   Size:        137     256
 
TatI w'GTAC_w

Cuts at:      0     210     393 
   Size:        210     183
 
Tth111I GACn'n_nGTC

Cuts at:      0      10     393 
   Size:         10     383
 
XmnI GAAnn'nnTTC

Cuts at:      0     257     393 
   Size:        257     136
 

 Enzymes that do cut:

     AleI     AvaI    BanII    BmgBI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspHI   BstAPI     BtgI     BtsI     EaeI     EarI     EciI  EcoICRI
     MslI   MspA1I     SacI     SapI     ScaI     SfcI     StyI     TatI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI