(Linear) (Six-base) MAPSORT of: KIBB3416.seq  Check: 1501  from: 1  to: 411



KIBB3416.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

ApaI G_GGCC'C

Cuts at:      0     243     411 
   Size:        243     168
 
BamHI G'GATC_C

Cuts at:      0     215     411 
   Size:        215     196
 
BanII G_rGCy'C

Cuts at:      0     157     243     411 
   Size:        157      86     168

  Fragments arranged by size: 

                 168     157      86
 
BclI T'GATC_A

Cuts at:      0     308     411 
   Size:        308     103
 
Bme1580I G_kGCm'C

Cuts at:      0     243     345     411 
   Size:        243     102      66

  Fragments arranged by size: 

                 243     102      66
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     208     411 
   Size:        208     203
 
BsaAI yAC'GTr

Cuts at:      0     346     411 
   Size:        346      65
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      10     411 
   Size:         10     401
 
BseYI C'CCAG_C

Cuts at:      0     150     411 
   Size:        150     261
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     411 
   Size:         60     351
 
BsiEI CG_ry'CG

Cuts at:      0      88     411 
   Size:         88     323
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     243     345     411 
   Size:        157      86     102      66

  Fragments arranged by size: 

                 157     102      86      66
 
BsrDI GCAATG_nn'

Cuts at:      0     166     411 
   Size:        166     245
 
BstYI r'GATC_y

Cuts at:      0     215     411 
   Size:        215     196
 
BtgI C'CryG_G

Cuts at:      0      36     411 
   Size:         36     375
 
EaeI y'GGCC_r

Cuts at:      0      85     132     411 
   Size:         85      47     279

  Fragments arranged by size: 

                 279      85      47
 
EagI C'GGCC_G

Cuts at:      0      85     411 
   Size:         85     326
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      46     411 
   Size:         46     365
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      25     411 
   Size:         25     386
 
EcoO109I rG'GnC_Cy

Cuts at:      0     239     411 
   Size:        239     172
 
HindIII A'AGCT_T

Cuts at:      0     265     411 
   Size:        265     146
 
MspA1I CmG'CkG

Cuts at:      0     150     411 
   Size:        150     261
 
PasI CC'CwG_GG

Cuts at:      0      66     125     411 
   Size:         66      59     286

  Fragments arranged by size: 

                 286      66      59
 
PmlI CAC'GTG

Cuts at:      0     346     411 
   Size:        346      65
 
PspOMI G'GGCC_C

Cuts at:      0     239     411 
   Size:        239     172
 
PvuII CAG'CTG

Cuts at:      0     150     411 
   Size:        150     261
 
SfcI C'TryA_G

Cuts at:      0     386     401     411 
   Size:        386      15      10

  Fragments arranged by size: 

                 386      15      10
 
StuI AGG'CCT

Cuts at:      0      31     411 
   Size:         31     380
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      58     411 
   Size:         58     353
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     335     367     411 
   Size:        335      32      44

  Fragments arranged by size: 

                 335      44      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     266     411 
   Size:        266     145
 

 Enzymes that do cut:

     ApaI    BamHI    BanII     BclI Bme1580I     BplI    BsaAI    BseRI
    BseYI     BsgI    BsiEI Bsp1286I    BsrDI    BstYI     BtgI     EaeI
     EagI     EciI    EcoNI EcoO109I  HindIII   MspA1I     PasI     PmlI
   PspOMI    PvuII     SfcI     StuI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI  EcoICRI  Eco57MI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI