Restriction map for
pF1KB3414
(Linear) (Six-base) MAPSORT of: KIBB3414.seq Check: 8491 from: 1 to: 2628
KIBB3414.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 648 1078 2628
Size: 648 430 1550
Fragments arranged by size:
1550 648 430
AccI GT'mk_AC
Cuts at: 0 174 445 1340 2628
Size: 174 271 895 1288
Fragments arranged by size:
1288 895 271 174
Acc65I G'GTAC_C
Cuts at: 0 1142 2628
Size: 1142 1486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1340 1454 1669 1723 1747 2200 2564
Size: 875 465 114 215 54 24 453 364
Cuts at: 2564 2628
Size: 64
Fragments arranged by size:
875 465 453 364 215 114 64 54
24
AflIII A'CryG_T
Cuts at: 0 1095 2628
Size: 1095 1533
AhdI GACnn_n'nnGTC
Cuts at: 0 268 1740 2628
Size: 268 1472 888
Fragments arranged by size:
1472 888 268
AleI CACnn'nnGTG
Cuts at: 0 1903 2628
Size: 1903 725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1065 2628
Size: 1065 1563
AlwNI CAG_nnn'CTG
Cuts at: 0 720 963 1365 2139 2628
Size: 720 243 402 774 489
Fragments arranged by size:
774 720 489 402 243
ApoI r'AATT_y
Cuts at: 0 841 1301 1924 2094 2383 2628
Size: 841 460 623 170 289 245
Fragments arranged by size:
841 623 460 289 245 170
AseI AT'TA_AT
Cuts at: 0 1246 2628
Size: 1246 1382
AvaI C'yCGr_G
Cuts at: 0 19 2628
Size: 19 2609
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 680 713 943 976 2628
Size: 680 33 230 33 1652
Fragments arranged by size:
1652 680 230 33 33
BanI G'GyrC_C
Cuts at: 0 1142 2628
Size: 1142 1486
BanII G_rGCy'C
Cuts at: 0 1196 2628
Size: 1196 1432
BbsI GAAGACnn'nnnn_
Cuts at: 0 31 2566 2628
Size: 31 2535 62
Fragments arranged by size:
2535 62 31
BbvCI CC'TCA_GC
Cuts at: 0 392 2628
Size: 392 2236
BclI T'GATC_A
Cuts at: 0 8 1467 2628
Size: 8 1459 1161
Fragments arranged by size:
1459 1161 8
BglI GCCn_nnn'nGGC
Cuts at: 0 1888 2628
Size: 1888 740
BglII A'GATC_T
Cuts at: 0 1805 2628
Size: 1805 823
Bme1580I G_kGCm'C
Cuts at: 0 2040 2628
Size: 2040 588
BmrI ACTGGGnnnn_n'
Cuts at: 0 358 1190 1253 2628
Size: 358 832 63 1375
Fragments arranged by size:
1375 832 358 63
BmtI G_CTAG'C
Cuts at: 0 577 2628
Size: 577 2051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 94 1407 2435 2628
Size: 67 27 1313 1028 193
Fragments arranged by size:
1313 1028 193 67 27
Bpu10I CC'TnA_GC
Cuts at: 0 392 920 2281 2628
Size: 392 528 1361 347
Fragments arranged by size:
1361 528 392 347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1915 2628
Size: 1915 713
BsaI GGTCTCn'nnnn_
Cuts at: 0 1381 2628
Size: 1381 1247
BsaBI GATnn'nnATC
Cuts at: 0 1628 2423 2628
Size: 1628 795 205
Fragments arranged by size:
1628 795 205
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 18 48 1085 1115 2628
Size: 18 30 1037 30 1513
Fragments arranged by size:
1513 1037 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1587 2189 2628
Size: 1587 602 439
Fragments arranged by size:
1587 602 439
BseYI C'CCAG_C
Cuts at: 0 1412 1855 2628
Size: 1412 443 773
Fragments arranged by size:
1412 773 443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 2628
Size: 374 2254
BsiEI CG_ry'CG
Cuts at: 0 42 86 2628
Size: 42 44 2542
Fragments arranged by size:
2542 44 42
BsiHKAI G_wGCw'C
Cuts at: 0 434 926 954 1711 2628
Size: 434 492 28 757 917
Fragments arranged by size:
917 757 492 434 28
BsmI GAATG_Cn'
Cuts at: 0 591 2628
Size: 591 2037
Bsp1286I G_dGCh'C
Cuts at: 0 434 926 954 1196 1711 2040 2628
Size: 434 492 28 242 515 329 588
Fragments arranged by size:
588 515 492 434 329 242 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 648 1078 2628
Size: 104 544 430 1550
Fragments arranged by size:
1550 544 430 104
BsrDI GCAATG_nn'
Cuts at: 0 806 2408 2628
Size: 806 1602 220
Fragments arranged by size:
1602 806 220
BsrFI r'CCGG_y
Cuts at: 0 311 2628
Size: 311 2317
BsrGI T'GTAC_A
Cuts at: 0 2476 2628
Size: 2476 152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1459 2628
Size: 1459 1169
BstXI CCAn_nnnn'nTGG
Cuts at: 0 129 2413 2628
Size: 129 2284 215
Fragments arranged by size:
2284 215 129
BstYI r'GATC_y
Cuts at: 0 204 359 1805 2628
Size: 204 155 1446 823
Fragments arranged by size:
1446 823 204 155
Bsu36I CC'TnA_GG
Cuts at: 0 1570 2628
Size: 1570 1058
BtgI C'CryG_G
Cuts at: 0 86 2628
Size: 86 2542
BtsI GCAGTG_nn'
Cuts at: 0 1163 2628
Size: 1163 1465
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 90 125 2628
Size: 90 35 2503
Fragments arranged by size:
2503 90 35
DraI TTT'AAA
Cuts at: 0 1950 2184 2628
Size: 1950 234 444
Fragments arranged by size:
1950 444 234
DraIII CAC_nnn'GTG
Cuts at: 0 334 680 2628
Size: 334 346 1948
Fragments arranged by size:
1948 346 334
EaeI y'GGCC_r
Cuts at: 0 83 376 401 878 1064 2628
Size: 83 293 25 477 186 1564
Fragments arranged by size:
1564 477 293 186 83 25
EagI C'GGCC_G
Cuts at: 0 83 2628
Size: 83 2545
EarI CTCTTCn'nnn_
Cuts at: 0 545 1766 2219 2628
Size: 545 1221 453 409
Fragments arranged by size:
1221 545 453 409
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2565 2628
Size: 2565 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 94 875 1340 1407 1454 1669 1723
Size: 67 27 781 465 67 47 215 54
Cuts at: 1723 1747 2200 2435 2564 2628
Size: 24 453 235 129 64
Fragments arranged by size:
781 465 453 235 215 129 67 67
64 54 47 27 24
EcoO109I rG'GnC_Cy
Cuts at: 0 769 2628
Size: 769 1859
EcoRI G'AATT_C
Cuts at: 0 841 1924 2628
Size: 841 1083 704
Fragments arranged by size:
1083 841 704
EcoRV GAT'ATC
Cuts at: 0 213 2628
Size: 213 2415
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 18 50 254 286 619 651 1359 1391
Size: 18 32 204 32 333 32 708 32
Cuts at: 1391 1726 1758 2628
Size: 335 32 870
Fragments arranged by size:
870 708 335 333 204 32 32 32
32 32 18
HincII GTy'rAC
Cuts at: 0 446 2540 2628
Size: 446 2094 88
Fragments arranged by size:
2094 446 88
HindIII A'AGCT_T
Cuts at: 0 887 2628
Size: 887 1741
HpaI GTT'AAC
Cuts at: 0 2540 2628
Size: 2540 88
KpnI G_GTAC'C
Cuts at: 0 1146 2628
Size: 1146 1482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2072 2628
Size: 2072 556
MscI TGG'CCA
Cuts at: 0 378 403 880 1066 2628
Size: 378 25 477 186 1562
Fragments arranged by size:
1562 477 378 186 25
MslI CAynn'nnrTG
Cuts at: 0 1793 1903 2354 2432 2628
Size: 1793 110 451 78 196
Fragments arranged by size:
1793 451 196 110 78
MspA1I CmG'CkG
Cuts at: 0 169 396 1282 1855 2008 2082 2628
Size: 169 227 886 573 153 74 546
Fragments arranged by size:
886 573 546 227 169 153 74
NdeI CA'TA_TG
Cuts at: 0 1584 2628
Size: 1584 1044
NheI G'CTAG_C
Cuts at: 0 573 2628
Size: 573 2055
NspI r_CATG'y
Cuts at: 0 1099 2628
Size: 1099 1529
PciI A'CATG_T
Cuts at: 0 1095 2628
Size: 1095 1533
PfoI T'CCnGG_A
Cuts at: 0 71 138 2628
Size: 71 67 2490
Fragments arranged by size:
2490 71 67
PpuMI rG'GwC_Cy
Cuts at: 0 769 2628
Size: 769 1859
PshAI GACnn'nnGTC
Cuts at: 0 1654 2628
Size: 1654 974
PstI C_TGCA'G
Cuts at: 0 1455 2628
Size: 1455 1173
PvuI CG_AT'CG
Cuts at: 0 42 2628
Size: 42 2586
PvuII CAG'CTG
Cuts at: 0 169 396 1282 1855 2008 2082 2628
Size: 169 227 886 573 153 74 546
Fragments arranged by size:
886 573 546 227 169 153 74
SalI G'TCGA_C
Cuts at: 0 444 2628
Size: 444 2184
SapI GCTCTTCn'nnn_
Cuts at: 0 545 2628
Size: 545 2083
SexAI A'CCwGG_T
Cuts at: 0 1991 2628
Size: 1991 637
SfcI C'TryA_G
Cuts at: 0 955 1292 1357 1451 1538 2628
Size: 955 337 65 94 87 1090
Fragments arranged by size:
1090 955 337 94 87 65
SmlI C'TyrA_G
Cuts at: 0 19 1930 2628
Size: 19 1911 698
Fragments arranged by size:
1911 698 19
SpeI A'CTAG_T
Cuts at: 0 1212 1902 2628
Size: 1212 690 726
Fragments arranged by size:
1212 726 690
StuI AGG'CCT
Cuts at: 0 1945 2224 2628
Size: 1945 279 404
Fragments arranged by size:
1945 404 279
StyI C'CwwG_G
Cuts at: 0 1619 2628
Size: 1619 1009
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1906 2628
Size: 1906 722
TatI w'GTAC_w
Cuts at: 0 749 1869 1934 2476 2496 2628
Size: 749 1120 65 542 20 132
Fragments arranged by size:
1120 749 542 132 65 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 666 698 1085 1117 2628
Size: 666 32 387 32 1511
Fragments arranged by size:
1511 666 387 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 30 1087 2628
Size: 30 1057 1541
Fragments arranged by size:
1541 1057 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 371 1852 2628
Size: 108 263 1481 776
Fragments arranged by size:
1481 776 263 108
XhoI C'TCGA_G
Cuts at: 0 19 2628
Size: 19 2609
XmnI GAAnn'nnTTC
Cuts at: 0 386 2387 2628
Size: 386 2001 241
Fragments arranged by size:
2001 386 241
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AlwNI ApoI AseI AvaI BaeI BanI BanII BbsI
BbvCI BclI BglI BglII Bme1580I BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
DraIII EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV Hin4I HincII HindIII HpaI KpnI MmeI MscI
MslI MspA1I NdeI NheI NspI PciI PfoI PpuMI
PshAI PstI PvuI PvuII SalI SapI SexAI SfcI
SmlI SpeI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AloI ApaI ApaLI
AscI AsiSI AvrII BamHI BbeI BcgI BciVI BfrBI
BlpI BmgBI BplI BsaAI BsaHI BsaWI BsiWI BsmBI
BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI DrdI EcoICRI EcoNI FalI FseI FspI
FspAI HaeII KasI MfeI MluI NaeI NarI NcoI
NgoMIV NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SspI SwaI XbaI XmaI
ZraI