(Linear) (Six-base) MAPSORT of: KIBB3403.seq  Check: 6571  from: 1  to: 765



KIBB3403.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     523     751     765 
   Size:        279     244     228      14

  Fragments arranged by size: 

                 279     244     228      14
 
BanII G_rGCy'C

Cuts at:      0       7     765 
   Size:          7     758
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     675     765 
   Size:        675      90
 
BfrBI ATG'CAT

Cuts at:      0     364     765 
   Size:        364     401
 
BglII A'GATC_T

Cuts at:      0     298     765 
   Size:        298     467
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      26     765 
   Size:         26     739
 
Bpu10I CC'TnA_GC

Cuts at:      0     719     765 
   Size:        719      46
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      30      60     765 
   Size:         30      30     705

  Fragments arranged by size: 

                 705      30      30
 
BseYI C'CCAG_C

Cuts at:      0     644     765 
   Size:        644     121
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     765 
   Size:          7     758
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     765 
   Size:          7     758
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     226     565     765 
   Size:        226     339     200

  Fragments arranged by size: 

                 339     226     200
 
BstYI r'GATC_y

Cuts at:      0     298     765 
   Size:        298     467
 
EaeI y'GGCC_r

Cuts at:      0     347     765 
   Size:        347     418
 
EarI CTCTTCn'nnn_

Cuts at:      0      51     765 
   Size:         51     714
 
EcoICRI GAG'CTC

Cuts at:      0       5     765 
   Size:          5     760
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     523     751     765 
   Size:        279     244     228      14

  Fragments arranged by size: 

                 279     244     228      14
 
EcoRV GAT'ATC

Cuts at:      0     567     765 
   Size:        567     198
 
FspI TGC'GCA

Cuts at:      0     440     765 
   Size:        440     325
 
HindIII A'AGCT_T

Cuts at:      0     284     535     623     765 
   Size:        284     251      88     142

  Fragments arranged by size: 

                 284     251     142      88
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     212     314     765 
   Size:        201      11     102     451

  Fragments arranged by size: 

                 451     201     102      11
 
MscI TGG'CCA

Cuts at:      0     349     765 
   Size:        349     416
 
MslI CAynn'nnrTG

Cuts at:      0     355     765 
   Size:        355     410
 
MspA1I CmG'CkG

Cuts at:      0     644     765 
   Size:        644     121
 
NsiI A_TGCA'T

Cuts at:      0     366     765 
   Size:        366     399
 
NspI r_CATG'y

Cuts at:      0     220     364     765 
   Size:        220     144     401

  Fragments arranged by size: 

                 401     220     144
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     623     655     765 
   Size:        623      32     110

  Fragments arranged by size: 

                 623     110      32
 
PstI C_TGCA'G

Cuts at:      0     403     765 
   Size:        403     362
 
PvuII CAG'CTG

Cuts at:      0     644     765 
   Size:        644     121
 
SacI G_AGCT'C

Cuts at:      0       7     765 
   Size:          7     758
 
SfcI C'TryA_G

Cuts at:      0     375     399     765 
   Size:        375      24     366

  Fragments arranged by size: 

                 375     366      24
 
SphI G_CATG'C

Cuts at:      0     364     765 
   Size:        364     401
 
TatI w'GTAC_w

Cuts at:      0     103     600     765 
   Size:        103     497     165

  Fragments arranged by size: 

                 497     165     103
 
XmnI GAAnn'nnTTC

Cuts at:      0     680     765 
   Size:        680      85
 

 Enzymes that do cut:

     AcuI    BanII     BbsI    BfrBI    BglII     BmrI   Bpu10I    BsaXI
    BseYI  BsiHKAI Bsp1286I    BspMI    BstYI     EaeI     EarI  EcoICRI
  Eco57MI    EcoRV     FspI  HindIII     MmeI     MscI     MslI   MspA1I
     NsiI     NspI     PpiI     PstI    PvuII     SacI     SfcI     SphI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoNI EcoO109I    EcoRI     FalI     FseI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI