(Linear) (Six-base) MAPSORT of: KIBB3398.seq  Check: 6362  from: 1  to: 453



KIBB3398.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     453 
   Size:        275     178
 
AflII C'TTAA_G

Cuts at:      0     223     453 
   Size:        223     230
 
AhdI GACnn_n'nnGTC

Cuts at:      0      61     453 
   Size:         61     392
 
AlwNI CAG_nnn'CTG

Cuts at:      0     289     439     453 
   Size:        289     150      14

  Fragments arranged by size: 

                 289     150      14
 
ApoI r'AATT_y

Cuts at:      0     209     218     334     453 
   Size:        209       9     116     119

  Fragments arranged by size: 

                 209     119     116       9
 
AvaI C'yCGr_G

Cuts at:      0      19     453 
   Size:         19     434
 
BfrBI ATG'CAT

Cuts at:      0      12     453 
   Size:         12     441
 
BglII A'GATC_T

Cuts at:      0     258     453 
   Size:        258     195
 
BmgBI CAC'GTC

Cuts at:      0      96     453 
   Size:         96     357
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      30     453 
   Size:         30     423
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     453 
   Size:        325     128
 
BstBI TT'CG_AA

Cuts at:      0     294     453 
   Size:        294     159
 
BstYI r'GATC_y

Cuts at:      0     258     453 
   Size:        258     195
 
DraIII CAC_nnn'GTG

Cuts at:      0     175     453 
   Size:        175     278
 
EaeI y'GGCC_r

Cuts at:      0     119     453 
   Size:        119     334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     453 
   Size:        275     178
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     129     453 
   Size:         27     102     324

  Fragments arranged by size: 

                 324     102      27
 
EcoRI G'AATT_C

Cuts at:      0     218     453 
   Size:        218     235
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      47      79     453 
   Size:         47      32     374

  Fragments arranged by size: 

                 374      47      32
 
HincII GTy'rAC

Cuts at:      0      83     453 
   Size:         83     370
 
MfeI C'AATT_G

Cuts at:      0     411     453 
   Size:        411      42
 
MscI TGG'CCA

Cuts at:      0     121     453 
   Size:        121     332
 
NsiI A_TGCA'T

Cuts at:      0      14     453 
   Size:         14     439
 
NspI r_CATG'y

Cuts at:      0      12      16     453 
   Size:         12       4     437

  Fragments arranged by size: 

                 437      12       4
 
PacI TTA_AT'TAA

Cuts at:      0     275     453 
   Size:        275     178
 
PsiI TTA'TAA

Cuts at:      0     386     453 
   Size:        386      67
 
SfcI C'TryA_G

Cuts at:      0     431     453 
   Size:        431      22
 
SmaI CCC'GGG

Cuts at:      0      21     453 
   Size:         21     432
 
SmlI C'TyrA_G

Cuts at:      0     223     304     453 
   Size:        223      81     149

  Fragments arranged by size: 

                 223     149      81
 
SphI G_CATG'C

Cuts at:      0      12      16     453 
   Size:         12       4     437

  Fragments arranged by size: 

                 437      12       4
 
XmaI C'CCGG_G

Cuts at:      0      19     453 
   Size:         19     434
 

 Enzymes that do cut:

     AcuI    AflII     AhdI    AlwNI     ApoI     AvaI    BfrBI    BglII
    BmgBI     BmrI    BpuEI    BstBI    BstYI   DraIII     EaeI  Eco57MI
 EcoO109I    EcoRI    Hin4I   HincII     MfeI     MscI     NsiI     NspI
     PacI     PsiI     SfcI     SmaI     SmlI     SphI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI     BlpI Bme1580I     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI