Restriction map for
pF1KB3388
(Linear) (Six-base) MAPSORT of: KIBB3388.seq Check: 2778 from: 1 to: 609
KIBB3388.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 609
Size: 406 203
AhdI GACnn_n'nnGTC
Cuts at: 0 604 609
Size: 604 5
AlwNI CAG_nnn'CTG
Cuts at: 0 18 81 609
Size: 18 63 528
Fragments arranged by size:
528 63 18
ApaI G_GGCC'C
Cuts at: 0 225 609
Size: 225 384
AvaI C'yCGr_G
Cuts at: 0 200 609
Size: 200 409
BanI G'GyrC_C
Cuts at: 0 416 609
Size: 416 193
BanII G_rGCy'C
Cuts at: 0 225 609
Size: 225 384
BbeI G_GCGC'C
Cuts at: 0 420 609
Size: 420 189
BglI GCCn_nnn'nGGC
Cuts at: 0 67 609
Size: 67 542
Bme1580I G_kGCm'C
Cuts at: 0 225 609
Size: 225 384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 58 609
Size: 58 551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 609
Size: 487 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 355 571 609
Size: 289 66 216 38
Fragments arranged by size:
289 216 66 38
BsaHI Gr'CG_yC
Cuts at: 0 417 609
Size: 417 192
BsaWI w'CCGG_w
Cuts at: 0 503 609
Size: 503 106
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 609
Size: 484 125
BseYI C'CCAG_C
Cuts at: 0 84 226 609
Size: 84 142 383
Fragments arranged by size:
383 142 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 609
Size: 31 578
BsiWI C'GTAC_G
Cuts at: 0 105 609
Size: 105 504
BsmBI CGTCTCn'nnnn_
Cuts at: 0 307 609
Size: 307 302
Bsp1286I G_dGCh'C
Cuts at: 0 225 609
Size: 225 384
BspHI T'CATG_A
Cuts at: 0 521 609
Size: 521 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3 609
Size: 3 606
BsrBI CCG'CTC
Cuts at: 0 292 609
Size: 292 317
BssSI C'ACGA_G
Cuts at: 0 427 609
Size: 427 182
BstYI r'GATC_y
Cuts at: 0 494 609
Size: 494 115
BtgI C'CryG_G
Cuts at: 0 594 609
Size: 594 15
BtsI GCAGTG_nn'
Cuts at: 0 458 609
Size: 458 151
EaeI y'GGCC_r
Cuts at: 0 59 609
Size: 59 550
EarI CTCTTCn'nnn_
Cuts at: 0 469 609
Size: 469 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 258 609
Size: 19 239 351
Fragments arranged by size:
351 239 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 487 609
Size: 406 81 122
Fragments arranged by size:
406 122 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 200 609
Size: 200 409
EcoO109I rG'GnC_Cy
Cuts at: 0 15 205 222 582 609
Size: 15 190 17 360 27
Fragments arranged by size:
360 190 27 17 15
HaeII r_GCGC'y
Cuts at: 0 420 609
Size: 420 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 590 609
Size: 590 19
KasI G'GCGC_C
Cuts at: 0 416 609
Size: 416 193
NarI GG'CG_CC
Cuts at: 0 417 609
Size: 417 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 45 609
Size: 45 564
PpuMI rG'GwC_Cy
Cuts at: 0 15 582 609
Size: 15 567 27
Fragments arranged by size:
567 27 15
PspOMI G'GGCC_C
Cuts at: 0 221 609
Size: 221 388
RsrII CG'GwC_CG
Cuts at: 0 245 609
Size: 245 364
SfcI C'TryA_G
Cuts at: 0 141 609
Size: 141 468
SfoI GGC'GCC
Cuts at: 0 418 609
Size: 418 191
SmlI C'TyrA_G
Cuts at: 0 304 370 586 609
Size: 304 66 216 23
Fragments arranged by size:
304 216 66 23
Enzymes that do cut:
AcuI AhdI AlwNI ApaI AvaI BanI BanII BbeI
BglI Bme1580I BplI BpmI BpuEI BsaHI BsaWI BseRI
BseYI BsgI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI
BssSI BstYI BtgI BtsI EaeI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI NarI PflMI PpuMI
PspOMI RsrII SfcI SfoI SmlI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I
BsaI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsmI BspEI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI