(Linear) (Six-base) MAPSORT of: KIBB3356.seq  Check: 1395  from: 1  to: 2577



KIBB3356.orf

 With 182 enzymes: SgfI * 

                        December 26, 2011 13:17  ..

AccI GT'mk_AC

Cuts at:      0     534    2577 
   Size:        534    2043
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1751    2333    2577 
   Size:       1751     582     244

  Fragments arranged by size: 

                1751     582     244
 
AfeI AGC'GCT

Cuts at:      0    2482    2577 
   Size:       2482      95
 
AflIII A'CryG_T

Cuts at:      0     772    2577 
   Size:        772    1805
 
ApaLI G'TGCA_C

Cuts at:      0    2183    2577 
   Size:       2183     394
 
ApoI r'AATT_y

Cuts at:      0    1249    1362    2577 
   Size:       1249     113    1215

  Fragments arranged by size: 

                1249    1215     113
 
AvaI C'yCGr_G

Cuts at:      0    2099    2577 
   Size:       2099     478
 
AvrII C'CTAG_G

Cuts at:      0     741    2577 
   Size:        741    1836
 
BamHI G'GATC_C

Cuts at:      0     295    2577 
   Size:        295    2282
 
BclI T'GATC_A

Cuts at:      0     504     876    2577 
   Size:        504     372    1701

  Fragments arranged by size: 

                1701     504     372
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1322    2577 
   Size:       1322    1255
 
BglII A'GATC_T

Cuts at:      0     593    2577 
   Size:        593    1984
 
Bme1580I G_kGCm'C

Cuts at:      0    2187    2577 
   Size:       2187     390
 
BmgBI CAC'GTC

Cuts at:      0    1837    2167    2577 
   Size:       1837     330     410

  Fragments arranged by size: 

                1837     410     330
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1164    1450    2577 
   Size:       1164     286    1127

  Fragments arranged by size: 

                1164    1127     286
 
BmtI G_CTAG'C

Cuts at:      0    1741    2577 
   Size:       1741     836
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     134    2358    2465    2577 
   Size:         33     101    2224     107     112

  Fragments arranged by size: 

                2224     112     107     101      33
 
Bpu10I CC'TnA_GC

Cuts at:      0    1661    2577 
   Size:       1661     916
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1400    2577 
   Size:       1400    1177
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1866    2577 
   Size:       1866     711
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1865    1895    2577 
   Size:       1865      30     682

  Fragments arranged by size: 

                1865     682      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     979    1625    2577 
   Size:        979     646     952

  Fragments arranged by size: 

                 979     952     646
 
BseYI C'CCAG_C

Cuts at:      0      67    2213    2577 
   Size:         67    2146     364

  Fragments arranged by size: 

                2146     364      67
 
BsiHKAI G_wGCw'C

Cuts at:      0      19      90     168    2187    2577 
   Size:         19      71      78    2019     390

  Fragments arranged by size: 

                2019     390      78      71      19
 
BsmI GAATG_Cn'

Cuts at:      0    1083    2577 
   Size:       1083    1494
 
Bsp1286I G_dGCh'C

Cuts at:      0      19      90     168    2187    2577 
   Size:         19      71      78    2019     390

  Fragments arranged by size: 

                2019     390      78      71      19
 
BsrDI GCAATG_nn'

Cuts at:      0     787    1197    1311    2577 
   Size:        787     410     114    1266

  Fragments arranged by size: 

                1266     787     410     114
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     545    1114    2577 
   Size:        545     569    1463

  Fragments arranged by size: 

                1463     569     545
 
BstYI r'GATC_y

Cuts at:      0     295     593    2344    2577 
   Size:        295     298    1751     233

  Fragments arranged by size: 

                1751     298     295     233
 
BstZ17I GTA'TAC

Cuts at:      0     535    2577 
   Size:        535    2042
 
BtgI C'CryG_G

Cuts at:      0      31    1129    1946    2577 
   Size:         31    1098     817     631

  Fragments arranged by size: 

                1098     817     631      31
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1454    2577 
   Size:       1454    1123
 
BtsI GCAGTG_nn'

Cuts at:      0    1481    2498    2577 
   Size:       1481    1017      79

  Fragments arranged by size: 

                1481    1017      79
 
DraI TTT'AAA

Cuts at:      0     710     912    2577 
   Size:        710     202    1665

  Fragments arranged by size: 

                1665     710     202
 
DraIII CAC_nnn'GTG

Cuts at:      0     172    2577 
   Size:        172    2405
 
EarI CTCTTCn'nnn_

Cuts at:      0     399     584    1620    1977    2292    2387    2577 
   Size:        399     185    1036     357     315      95     190

  Fragments arranged by size: 

                1036     399     357     315     190     185      95
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     134    1751    2333    2358    2465    2577 
   Size:         33     101    1617     582      25     107     112

  Fragments arranged by size: 

                1617     582     112     107     101      33      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1784    2577 
   Size:       1784     793
 
HaeII r_GCGC'y

Cuts at:      0    2484    2577 
   Size:       2484      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     424     456    2380    2412    2577 
   Size:        424      32    1924      32     165

  Fragments arranged by size: 

                1924     424     165      32      32
 
HincII GTy'rAC

Cuts at:      0     131     323     995    2577 
   Size:        131     192     672    1582

  Fragments arranged by size: 

                1582     672     192     131
 
HindIII A'AGCT_T

Cuts at:      0     979    1549    2577 
   Size:        979     570    1028

  Fragments arranged by size: 

                1028     979     570
 
MfeI C'AATT_G

Cuts at:      0     849    1224    1651    2577 
   Size:        849     375     427     926

  Fragments arranged by size: 

                 926     849     427     375
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     643    2577 
   Size:        643    1934
 
MslI CAynn'nnrTG

Cuts at:      0     428    1061    1536    2577 
   Size:        428     633     475    1041

  Fragments arranged by size: 

                1041     633     475     428
 
MspA1I CmG'CkG

Cuts at:      0      33    2577 
   Size:         33    2544
 
NcoI C'CATG_G

Cuts at:      0    1129    2577 
   Size:       1129    1448
 
NheI G'CTAG_C

Cuts at:      0    1737    2577 
   Size:       1737     840
 
NspI r_CATG'y

Cuts at:      0     776    2075    2577 
   Size:        776    1299     502

  Fragments arranged by size: 

                1299     776     502
 
PciI A'CATG_T

Cuts at:      0     772    2577 
   Size:        772    1805
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     775    2577 
   Size:        775    1802
 
PfoI T'CCnGG_A

Cuts at:      0     840    2442    2577 
   Size:        840    1602     135

  Fragments arranged by size: 

                1602     840     135
 
SacII CC_GC'GG

Cuts at:      0      34    2577 
   Size:         34    2543
 
SexAI A'CCwGG_T

Cuts at:      0     856    2577 
   Size:        856    1721
 
SfcI C'TryA_G

Cuts at:      0     225    2577 
   Size:        225    2352
 
SmlI C'TyrA_G

Cuts at:      0    1415    2577 
   Size:       1415    1162
 
SspI AAT'ATT

Cuts at:      0     345     793    2577 
   Size:        345     448    1784

  Fragments arranged by size: 

                1784     448     345
 
StyI C'CwwG_G

Cuts at:      0     741    1129    2577 
   Size:        741     388    1448

  Fragments arranged by size: 

                1448     741     388
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1392    2577 
   Size:       1392    1185
 
TatI w'GTAC_w

Cuts at:      0    1540    2577 
   Size:       1540    1037
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2114    2146    2577 
   Size:       2114      32     431

  Fragments arranged by size: 

                2114     431      32
 
XbaI T'CTAG_A

Cuts at:      0     442    2577 
   Size:        442    2135
 
XmnI GAAnn'nnTTC

Cuts at:      0    1250    1688    2577 
   Size:       1250     438     889

  Fragments arranged by size: 

                1250     889     438
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII    ApaLI     ApoI     AvaI    AvrII
    BamHI     BclI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BsrDI    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     DraI   DraIII     EarI  Eco57MI    EcoNI    HaeII    Hin4I
   HincII  HindIII     MfeI     MmeI     MslI   MspA1I     NcoI     NheI
     NspI     PciI    PflMI     PfoI    SacII    SexAI     SfcI     SmlI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     BaeI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BlpI     BplI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I     ClaI    CspCI
     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MluI
     MscI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     NsiI
     PacI     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
  Tth111I     XcmI     XhoI     XmaI     ZraI