Restriction map for
pF1KB3348
(Linear) (Six-base) MAPSORT of: KIBB3348.seq Check: 9337 from: 1 to: 2109
KIBB3348.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 978 2109
Size: 978 1131
AatII G_ACGT'C
Cuts at: 0 1418 2109
Size: 1418 691
AccI GT'mk_AC
Cuts at: 0 1280 2109
Size: 1280 829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 269 445 2109
Size: 52 217 176 1664
Fragments arranged by size:
1664 217 176 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2063 2109
Size: 2063 46
AlwNI CAG_nnn'CTG
Cuts at: 0 1167 1275 1537 1554 1581 1932 2016 2100
Size: 1167 108 262 17 27 351 84 84
Cuts at: 2100 2109
Size: 9
Fragments arranged by size:
1167 351 262 108 84 84 27 17
9
ApaI G_GGCC'C
Cuts at: 0 76 1860 2109
Size: 76 1784 249
Fragments arranged by size:
1784 249 76
ApoI r'AATT_y
Cuts at: 0 1405 1944 2109
Size: 1405 539 165
Fragments arranged by size:
1405 539 165
AseI AT'TA_AT
Cuts at: 0 502 2109
Size: 502 1607
AvaI C'yCGr_G
Cuts at: 0 1853 2109
Size: 1853 256
BamHI G'GATC_C
Cuts at: 0 671 2109
Size: 671 1438
BanII G_rGCy'C
Cuts at: 0 76 243 332 361 372 763 1211 1860
Size: 76 167 89 29 11 391 448 649
Cuts at: 1860 2109
Size: 249
Fragments arranged by size:
649 448 391 249 167 89 76 29
11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 617 651 1006 1040 2109
Size: 617 34 355 34 1069
Fragments arranged by size:
1069 617 355 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 759 2109
Size: 759 1350
BglI GCCn_nnn'nGGC
Cuts at: 0 632 2109
Size: 632 1477
BlpI GC'TnA_GC
Cuts at: 0 331 757 856 1174 1300 2109
Size: 331 426 99 318 126 809
Fragments arranged by size:
809 426 331 318 126 99
Bme1580I G_kGCm'C
Cuts at: 0 76 1860 1908 2109
Size: 76 1784 48 201
Fragments arranged by size:
1784 201 76 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 765 2109
Size: 765 1344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 386 417 2008 2041 2109
Size: 139 247 31 1591 33 68
Fragments arranged by size:
1591 247 139 68 33 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1441 2109
Size: 80 1361 668
Fragments arranged by size:
1361 668 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 12 1100 1862 2109
Size: 12 1088 762 247
Fragments arranged by size:
1088 762 247 12
BsaAI yAC'GTr
Cuts at: 0 262 2109
Size: 262 1847
BsaBI GATnn'nnATC
Cuts at: 0 532 2109
Size: 532 1577
BsaHI Gr'CG_yC
Cuts at: 0 1415 2109
Size: 1415 694
BsaWI w'CCGG_w
Cuts at: 0 1669 2109
Size: 1669 440
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 182 212 863 893 910 940 2109
Size: 182 30 651 30 17 30 1169
Fragments arranged by size:
1169 651 182 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 644 681 1057 2109
Size: 442 202 37 376 1052
Fragments arranged by size:
1052 442 376 202 37
BseYI C'CCAG_C
Cuts at: 0 135 174 270 2051 2109
Size: 135 39 96 1781 58
Fragments arranged by size:
1781 135 96 58 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1132 1180 1288 1609 1936 2109
Size: 877 255 48 108 321 327 173
Fragments arranged by size:
877 327 321 255 173 108 48
BsiHKAI G_wGCw'C
Cuts at: 0 267 763 812 1211 1457 1531 2024 2109
Size: 267 496 49 399 246 74 493 85
Fragments arranged by size:
496 493 399 267 246 85 74 49
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1422 2109
Size: 1422 687
Bsp1286I G_dGCh'C
Cuts at: 0 76 243 267 332 361 372 763 812
Size: 76 167 24 65 29 11 391 49
Cuts at: 812 1211 1457 1531 1860 1908 2024 2109
Size: 399 246 74 329 48 116 85
Fragments arranged by size:
399 391 329 246 167 116 85 76
74 65 49 48 29 24 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 452 978 1136 1631 2109
Size: 452 526 158 495 478
Fragments arranged by size:
526 495 478 452 158
BsrBI CCG'CTC
Cuts at: 0 204 2109
Size: 204 1905
BsrDI GCAATG_nn'
Cuts at: 0 1311 1688 1847 2109
Size: 1311 377 159 262
Fragments arranged by size:
1311 377 262 159
BsrGI T'GTAC_A
Cuts at: 0 926 2109
Size: 926 1183
BssHII G'CGCG_C
Cuts at: 0 2001 2109
Size: 2001 108
BstAPI GCAn_nnn'nTGC
Cuts at: 0 993 1194 1932 2109
Size: 993 201 738 177
Fragments arranged by size:
993 738 201 177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 113 2109
Size: 113 1996
BstYI r'GATC_y
Cuts at: 0 671 959 2012 2109
Size: 671 288 1053 97
Fragments arranged by size:
1053 671 288 97
BstZ17I GTA'TAC
Cuts at: 0 1281 2109
Size: 1281 828
BtgI C'CryG_G
Cuts at: 0 1873 1950 2109
Size: 1873 77 159
Fragments arranged by size:
1873 159 77
BtsI GCAGTG_nn'
Cuts at: 0 190 977 1614 1924 2109
Size: 190 787 637 310 185
Fragments arranged by size:
787 637 310 190 185
DraI TTT'AAA
Cuts at: 0 797 2109
Size: 797 1312
DrdI GACnn_nn'nnGTC
Cuts at: 0 1876 2109
Size: 1876 233
EaeI y'GGCC_r
Cuts at: 0 212 974 1844 2109
Size: 212 762 870 265
Fragments arranged by size:
870 762 265 212
EarI CTCTTCn'nnn_
Cuts at: 0 598 1519 2109
Size: 598 921 590
Fragments arranged by size:
921 598 590
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1116 2109
Size: 1116 993
EcoICRI GAG'CTC
Cuts at: 0 761 1209 2109
Size: 761 448 900
Fragments arranged by size:
900 761 448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 139 269 386 417 445 2008 2041
Size: 52 87 130 117 31 28 1563 33
Cuts at: 2041 2109
Size: 68
Fragments arranged by size:
1563 130 117 87 68 52 33 31
28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1891 2109
Size: 1891 218
EcoO109I rG'GnC_Cy
Cuts at: 0 72 73 125 254 708 1071 1507 1857
Size: 72 1 52 129 454 363 436 350
Cuts at: 1857 1878 1929 2070 2109
Size: 21 51 141 39
Fragments arranged by size:
454 436 363 350 141 129 72 52
51 39 21 1
EcoRI G'AATT_C
Cuts at: 0 1944 2109
Size: 1944 165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1090 2109
Size: 1090 1019
HaeII r_GCGC'y
Cuts at: 0 1501 2109
Size: 1501 608
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 863 895 2057 2089 2109
Size: 230 32 601 32 1162 32 20
Fragments arranged by size:
1162 601 230 32 32 32 20
HindIII A'AGCT_T
Cuts at: 0 2044 2109
Size: 2044 65
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 2109
Size: 987 1122
MscI TGG'CCA
Cuts at: 0 214 976 1846 2109
Size: 214 762 870 263
Fragments arranged by size:
870 762 263 214
MslI CAynn'nnrTG
Cuts at: 0 775 1754 2109
Size: 775 979 355
Fragments arranged by size:
979 775 355
MspA1I CmG'CkG
Cuts at: 0 335 916 1092 1818 1875 2097 2109
Size: 335 581 176 726 57 222 12
Fragments arranged by size:
726 581 335 222 176 57 12
NspI r_CATG'y
Cuts at: 0 900 2109
Size: 900 1209
PasI CC'CwG_GG
Cuts at: 0 1215 2109
Size: 1215 894
PflMI CCAn_nnn'nTGG
Cuts at: 0 1773 2016 2109
Size: 1773 243 93
Fragments arranged by size:
1773 243 93
PfoI T'CCnGG_A
Cuts at: 0 363 674 722 962 1766 1886 2024 2057
Size: 363 311 48 240 804 120 138 33
Cuts at: 2057 2109
Size: 52
Fragments arranged by size:
804 363 311 240 138 120 52 48
33
PmlI CAC'GTG
Cuts at: 0 262 2109
Size: 262 1847
PpuMI rG'GwC_Cy
Cuts at: 0 125 708 1507 1878 2070 2109
Size: 125 583 799 371 192 39
Fragments arranged by size:
799 583 371 192 125 39
PspOMI G'GGCC_C
Cuts at: 0 72 1856 2109
Size: 72 1784 253
Fragments arranged by size:
1784 253 72
PstI C_TGCA'G
Cuts at: 0 621 1019 1190 1320 1394 1550 1706 1937
Size: 621 398 171 130 74 156 156 231
Cuts at: 1937 1991 2042 2109
Size: 54 51 67
Fragments arranged by size:
621 398 231 171 156 156 130 74
67 54 51
PvuII CAG'CTG
Cuts at: 0 335 1092 1818 2109
Size: 335 757 726 291
Fragments arranged by size:
757 726 335 291
SacI G_AGCT'C
Cuts at: 0 763 1211 2109
Size: 763 448 898
Fragments arranged by size:
898 763 448
SacII CC_GC'GG
Cuts at: 0 1876 2109
Size: 1876 233
SanDI GG'GwC_CC
Cuts at: 0 125 1507 2109
Size: 125 1382 602
Fragments arranged by size:
1382 602 125
SapI GCTCTTCn'nnn_
Cuts at: 0 1519 2109
Size: 1519 590
SbfI CC_TGCA'GG
Cuts at: 0 1937 2109
Size: 1937 172
SexAI A'CCwGG_T
Cuts at: 0 389 2109
Size: 389 1720
SfcI C'TryA_G
Cuts at: 0 617 1015 1186 1316 1390 1546 1702 1933
Size: 617 398 171 130 74 156 156 231
Cuts at: 1933 1987 2038 2109
Size: 54 51 71
Fragments arranged by size:
617 398 231 171 156 156 130 74
71 54 51
SmaI CCC'GGG
Cuts at: 0 1855 2109
Size: 1855 254
SmlI C'TyrA_G
Cuts at: 0 95 1456 2109
Size: 95 1361 653
Fragments arranged by size:
1361 653 95
SpeI A'CTAG_T
Cuts at: 0 1900 2109
Size: 1900 209
SrfI GCCC'GGGC
Cuts at: 0 1855 2109
Size: 1855 254
StuI AGG'CCT
Cuts at: 0 1449 2109
Size: 1449 660
StyI C'CwwG_G
Cuts at: 0 292 2109
Size: 292 1817
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 481 1228 1358 2109
Size: 481 747 130 751
Fragments arranged by size:
751 747 481 130
TatI w'GTAC_w
Cuts at: 0 926 2109
Size: 926 1183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 182 214 1935 1967 2109
Size: 182 32 1721 32 142
Fragments arranged by size:
1721 182 142 32 32
XmaI C'CCGG_G
Cuts at: 0 1853 2109
Size: 1853 256
XmnI GAAnn'nnTTC
Cuts at: 0 950 1327 2109
Size: 950 377 782
Fragments arranged by size:
950 782 377
ZraI GAC'GTC
Cuts at: 0 1416 2109
Size: 1416 693
Enzymes that do cut:
AarI AatII AccI AcuI AlfI AlwNI ApaI ApoI
AseI AvaI BamHI BanII BcgI BciVI BglI BlpI
Bme1580I BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstXI BstYI
BstZ17I BtgI BtsI DraI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII MmeI MscI MslI MspA1I NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII
SanDI SapI SbfI SexAI SfcI SmaI SmlI SpeI
SrfI StuI StyI TaqII TatI TstI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI AscI AsiSI AvrII BaeI BanI BbeI
BbsI BbvCI BclI BfrBI BglII BmgBI BmrI BmtI
Bpu10I BsiEI BsiWI BsmI BspEI BspHI BsrFI BssSI
BstBI BstEII Bsu36I BtgZI ClaI CspCI DraIII EagI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SalI ScaI SfiI
SfoI SgfI SgrAI SnaBI SphI SspI SwaI Tth111I
XbaI XcmI XhoI