Restriction map for
pF1KB3316
(Linear) (Six-base) MAPSORT of: KIBB3316.seq Check: 2828 from: 1 to: 2217
KIBB3316.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 1314 1982 2217
Size: 1314 668 235
Fragments arranged by size:
1314 668 235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 437 899 1073 2217
Size: 146 291 462 174 1144
Fragments arranged by size:
1144 462 291 174 146
AfeI AGC'GCT
Cuts at: 0 63 2217
Size: 63 2154
AgeI A'CCGG_T
Cuts at: 0 1834 2217
Size: 1834 383
AhdI GACnn_n'nnGTC
Cuts at: 0 753 1962 2217
Size: 753 1209 255
Fragments arranged by size:
1209 753 255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 305 2217
Size: 305 1912
AlwNI CAG_nnn'CTG
Cuts at: 0 271 1998 2217
Size: 271 1727 219
Fragments arranged by size:
1727 271 219
ApoI r'AATT_y
Cuts at: 0 511 1016 1391 1508 1522 1670 1743 1906
Size: 511 505 375 117 14 148 73 163
Cuts at: 1906 1932 2217
Size: 26 285
Fragments arranged by size:
511 505 375 285 163 148 117 73
26 14
BanI G'GyrC_C
Cuts at: 0 2098 2217
Size: 2098 119
BanII G_rGCy'C
Cuts at: 0 52 2217
Size: 52 2165
BbsI GAAGACnn'nnnn_
Cuts at: 0 1708 2217
Size: 1708 509
BlpI GC'TnA_GC
Cuts at: 0 2177 2217
Size: 2177 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 192 2049 2217
Size: 192 1857 168
Fragments arranged by size:
1857 192 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 2217
Size: 692 1525
BsaI GGTCTCn'nnnn_
Cuts at: 0 323 1364 2217
Size: 323 1041 853
Fragments arranged by size:
1041 853 323
BsaWI w'CCGG_w
Cuts at: 0 1656 1834 2217
Size: 1656 178 383
Fragments arranged by size:
1656 383 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 1794 1824 2217
Size: 37 30 1727 30 393
Fragments arranged by size:
1727 393 37 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 121 470 2217
Size: 118 3 349 1747
Fragments arranged by size:
1747 349 118 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 2217
Size: 956 1261
BsiHKAI G_wGCw'C
Cuts at: 0 2018 2217
Size: 2018 199
BsmI GAATG_Cn'
Cuts at: 0 863 905 1112 2217
Size: 863 42 207 1105
Fragments arranged by size:
1105 863 207 42
Bsp1286I G_dGCh'C
Cuts at: 0 52 2018 2217
Size: 52 1966 199
Fragments arranged by size:
1966 199 52
BspEI T'CCGG_A
Cuts at: 0 1656 2217
Size: 1656 561
BspHI T'CATG_A
Cuts at: 0 1721 2217
Size: 1721 496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1044 2217
Size: 1044 1173
BsrBI CCG'CTC
Cuts at: 0 1705 2165 2217
Size: 1705 460 52
Fragments arranged by size:
1705 460 52
BsrFI r'CCGG_y
Cuts at: 0 1834 2217
Size: 1834 383
BsrGI T'GTAC_A
Cuts at: 0 1209 2217
Size: 1209 1008
BstBI TT'CG_AA
Cuts at: 0 1668 2217
Size: 1668 549
BstYI r'GATC_y
Cuts at: 0 189 2217
Size: 189 2028
BstZ17I GTA'TAC
Cuts at: 0 1315 2217
Size: 1315 902
BtgI C'CryG_G
Cuts at: 0 25 2102 2217
Size: 25 2077 115
Fragments arranged by size:
2077 115 25
BtsI GCAGTG_nn'
Cuts at: 0 1349 1736 2217
Size: 1349 387 481
Fragments arranged by size:
1349 481 387
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 851 886 2217
Size: 851 35 1331
Fragments arranged by size:
1331 851 35
DraI TTT'AAA
Cuts at: 0 212 1032 1766 2217
Size: 212 820 734 451
Fragments arranged by size:
820 734 451 212
DraIII CAC_nnn'GTG
Cuts at: 0 1123 1918 2217
Size: 1123 795 299
Fragments arranged by size:
1123 795 299
EarI CTCTTCn'nnn_
Cuts at: 0 1005 2217
Size: 1005 1212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 1013 2217
Size: 34 979 1204
Fragments arranged by size:
1204 979 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 437 692 899 1073 2217
Size: 146 291 255 207 174 1144
Fragments arranged by size:
1144 291 255 207 174 146
EcoNI CCTnn'n_nnAGG
Cuts at: 0 803 2217
Size: 803 1414
EcoO109I rG'GnC_Cy
Cuts at: 0 1956 2217
Size: 1956 261
EcoRI G'AATT_C
Cuts at: 0 1932 2217
Size: 1932 285
EcoRV GAT'ATC
Cuts at: 0 360 2217
Size: 360 1857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 868 2217
Size: 264 604 1349
Fragments arranged by size:
1349 604 264
HaeII r_GCGC'y
Cuts at: 0 65 2217
Size: 65 2152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 739 771 1948 1980 1992 2024
Size: 37 32 670 32 1177 32 12 32
Cuts at: 2024 2217
Size: 193
Fragments arranged by size:
1177 670 193 37 32 32 32 32
12
HincII GTy'rAC
Cuts at: 0 626 2217
Size: 626 1591
HpaI GTT'AAC
Cuts at: 0 626 2217
Size: 626 1591
MfeI C'AATT_G
Cuts at: 0 1217 2217
Size: 1217 1000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 2217
Size: 80 2137
MspA1I CmG'CkG
Cuts at: 0 6 15 1698 2181 2217
Size: 6 9 1683 483 36
Fragments arranged by size:
1683 483 36 9 6
NcoI C'CATG_G
Cuts at: 0 2102 2217
Size: 2102 115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 320 352 475 507 2217
Size: 320 32 123 32 1710
Fragments arranged by size:
1710 320 123 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1956 2217
Size: 1956 261
PvuII CAG'CTG
Cuts at: 0 15 1698 2217
Size: 15 1683 519
Fragments arranged by size:
1683 519 15
SexAI A'CCwGG_T
Cuts at: 0 1958 2217
Size: 1958 259
SspI AAT'ATT
Cuts at: 0 1883 2217
Size: 1883 334
StuI AGG'CCT
Cuts at: 0 1050 2217
Size: 1050 1167
StyI C'CwwG_G
Cuts at: 0 804 2026 2102 2217
Size: 804 1222 76 115
Fragments arranged by size:
1222 804 115 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 347 2217
Size: 347 1870
TatI w'GTAC_w
Cuts at: 0 1209 2217
Size: 1209 1008
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 785 817 1445 1477 1794 1826 2217
Size: 785 32 628 32 317 32 391
Fragments arranged by size:
785 628 391 317 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1394 1583 1664 2217
Size: 1394 189 81 553
Fragments arranged by size:
1394 553 189 81
Enzymes that do cut:
AccI AcuI AfeI AgeI AhdI AlfI AlwNI ApoI
BanI BanII BbsI BlpI BmrI BpmI BsaI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BstBI BstYI BstZ17I BtgI
BtsI CspCI DraI DraIII EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI
MfeI MmeI MspA1I NcoI PpiI PpuMI PvuII SexAI
SspI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaHI BseYI BsiEI BsiWI BsmBI BsrDI BssHII
BssSI BstAPI BstEII BstXI Bsu36I BtgZI ClaI DrdI
EaeI EagI EcoICRI FseI FspI FspAI HindIII KasI
KpnI MluI MscI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI