Restriction map for
pF1KB3311
(Linear) (Six-base) MAPSORT of: KIBB3311.seq Check: 2519 from: 1 to: 1878
KIBB3311.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AatII G_ACGT'C
Cuts at: 0 1020 1878
Size: 1020 858
AccI GT'mk_AC
Cuts at: 0 1007 1878
Size: 1007 871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1487 1495 1831 1878
Size: 1487 8 336 47
Fragments arranged by size:
1487 336 47 8
AflIII A'CryG_T
Cuts at: 0 871 1878
Size: 871 1007
AhdI GACnn_n'nnGTC
Cuts at: 0 1827 1878
Size: 1827 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 983 1878
Size: 983 895
AlwNI CAG_nnn'CTG
Cuts at: 0 1256 1291 1349 1578 1725 1878
Size: 1256 35 58 229 147 153
Fragments arranged by size:
1256 229 153 147 58 35
ApoI r'AATT_y
Cuts at: 0 1566 1878
Size: 1566 312
AvaI C'yCGr_G
Cuts at: 0 195 1878
Size: 195 1683
BanI G'GyrC_C
Cuts at: 0 270 486 1753 1878
Size: 270 216 1267 125
Fragments arranged by size:
1267 270 216 125
BanII G_rGCy'C
Cuts at: 0 853 1141 1733 1878
Size: 853 288 592 145
Fragments arranged by size:
853 592 288 145
BbeI G_GCGC'C
Cuts at: 0 274 490 1878
Size: 274 216 1388
Fragments arranged by size:
1388 274 216
BbsI GAAGACnn'nnnn_
Cuts at: 0 1826 1878
Size: 1826 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 464 498 1496 1530 1878
Size: 464 34 998 34 348
Fragments arranged by size:
998 464 348 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1350 1878
Size: 1350 528
BclI T'GATC_A
Cuts at: 0 248 1625 1878
Size: 248 1377 253
Fragments arranged by size:
1377 253 248
BglI GCCn_nnn'nGGC
Cuts at: 0 617 748 760 812 1878
Size: 617 131 12 52 1066
Fragments arranged by size:
1066 617 131 52 12
BglII A'GATC_T
Cuts at: 0 1236 1775 1878
Size: 1236 539 103
Fragments arranged by size:
1236 539 103
BlpI GC'TnA_GC
Cuts at: 0 661 1292 1878
Size: 661 631 586
Fragments arranged by size:
661 631 586
Bme1580I G_kGCm'C
Cuts at: 0 871 1758 1878
Size: 871 887 120
Fragments arranged by size:
887 871 120
BmgBI CAC'GTC
Cuts at: 0 158 1878
Size: 158 1720
BmrI ACTGGGnnnn_n'
Cuts at: 0 997 1878
Size: 997 881
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 1878
Size: 210 1668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 338 345 1747 1777 1878
Size: 20 318 7 1402 30 101
Fragments arranged by size:
1402 318 101 30 20 7
Bpu10I CC'TnA_GC
Cuts at: 0 1599 1878
Size: 1599 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 733 1878
Size: 196 537 1145
Fragments arranged by size:
1145 537 196
BsaAI yAC'GTr
Cuts at: 0 872 1878
Size: 872 1006
BsaHI Gr'CG_yC
Cuts at: 0 271 487 1017 1878
Size: 271 216 530 861
Fragments arranged by size:
861 530 271 216
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1098 1101 1128 1131 1878
Size: 1098 3 27 3 747
Fragments arranged by size:
1098 747 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 1122 1169 1878
Size: 274 848 47 709
Fragments arranged by size:
848 709 274 47
BseYI C'CCAG_C
Cuts at: 0 166 274 385 607 672 810 823 841
Size: 166 108 111 222 65 138 13 18
Cuts at: 841 1349 1878
Size: 508 529
Fragments arranged by size:
529 508 222 166 138 111 108 65
18 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1764 1878
Size: 979 785 114
Fragments arranged by size:
979 785 114
BsiEI CG_ry'CG
Cuts at: 0 617 650 1878
Size: 617 33 1228
Fragments arranged by size:
1228 617 33
BsiHKAI G_wGCw'C
Cuts at: 0 853 1878
Size: 853 1025
BsiWI C'GTAC_G
Cuts at: 0 725 1878
Size: 725 1153
BsmI GAATG_Cn'
Cuts at: 0 1203 1878
Size: 1203 675
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1008 1878
Size: 1008 870
Bsp1286I G_dGCh'C
Cuts at: 0 853 871 1141 1733 1758 1878
Size: 853 18 270 592 25 120
Fragments arranged by size:
853 592 270 120 25 18
BspHI T'CATG_A
Cuts at: 0 89 1878
Size: 89 1789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 931 1878
Size: 931 947
BsrBI CCG'CTC
Cuts at: 0 567 1878
Size: 567 1311
BsrDI GCAATG_nn'
Cuts at: 0 1494 1878
Size: 1494 384
BsrFI r'CCGG_y
Cuts at: 0 435 550 616 1878
Size: 435 115 66 1262
Fragments arranged by size:
1262 435 115 66
BssHII G'CGCG_C
Cuts at: 0 554 556 1878
Size: 554 2 1322
Fragments arranged by size:
1322 554 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 810 1749 1878
Size: 810 939 129
Fragments arranged by size:
939 810 129
BstYI r'GATC_y
Cuts at: 0 1236 1775 1878
Size: 1236 539 103
Fragments arranged by size:
1236 539 103
BtgI C'CryG_G
Cuts at: 0 80 116 542 689 1878
Size: 80 36 426 147 1189
Fragments arranged by size:
1189 426 147 80 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 483 1878
Size: 483 1395
BtsI GCAGTG_nn'
Cuts at: 0 475 1460 1878
Size: 475 985 418
Fragments arranged by size:
985 475 418
DraI TTT'AAA
Cuts at: 0 1697 1878
Size: 1697 181
EaeI y'GGCC_r
Cuts at: 0 548 614 621 647 740 1878
Size: 548 66 7 26 93 1138
Fragments arranged by size:
1138 548 93 66 26 7
EagI C'GGCC_G
Cuts at: 0 614 647 1878
Size: 614 33 1231
Fragments arranged by size:
1231 614 33
EarI CTCTTCn'nnn_
Cuts at: 0 574 1661 1861 1878
Size: 574 1087 200 17
Fragments arranged by size:
1087 574 200 17
EcoICRI GAG'CTC
Cuts at: 0 851 1878
Size: 851 1027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 338 345 1487 1495 1747 1777 1831
Size: 20 318 7 1142 8 252 30 54
Cuts at: 1831 1878
Size: 47
Fragments arranged by size:
1142 318 252 54 47 30 20 8
7
EcoO109I rG'GnC_Cy
Cuts at: 0 1746 1878
Size: 1746 132
EcoRV GAT'ATC
Cuts at: 0 1590 1878
Size: 1590 288
HaeII r_GCGC'y
Cuts at: 0 274 490 1878
Size: 274 216 1388
Fragments arranged by size:
1388 274 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 1101 1130 1133 1185 1217 1433 1465
Size: 1098 3 29 3 52 32 216 32
Cuts at: 1465 1813 1845 1878
Size: 348 32 33
Fragments arranged by size:
1098 348 216 52 33 32 32 32
29 3 3
KasI G'GCGC_C
Cuts at: 0 270 486 1878
Size: 270 216 1392
Fragments arranged by size:
1392 270 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1044 1878
Size: 1044 834
MscI TGG'CCA
Cuts at: 0 742 1878
Size: 742 1136
MslI CAynn'nnrTG
Cuts at: 0 394 1878
Size: 394 1484
MspA1I CmG'CkG
Cuts at: 0 240 513 652 672 691 738 759 1878
Size: 240 273 139 20 19 47 21 1119
Fragments arranged by size:
1119 273 240 139 47 21 20 19
NaeI GCC'GGC
Cuts at: 0 437 552 618 1878
Size: 437 115 66 1260
Fragments arranged by size:
1260 437 115 66
NarI GG'CG_CC
Cuts at: 0 271 487 1878
Size: 271 216 1391
Fragments arranged by size:
1391 271 216
NcoI C'CATG_G
Cuts at: 0 116 1878
Size: 116 1762
NgoMIV G'CCGG_C
Cuts at: 0 435 550 616 1878
Size: 435 115 66 1262
Fragments arranged by size:
1262 435 115 66
NspI r_CATG'y
Cuts at: 0 1300 1878
Size: 1300 578
PasI CC'CwG_GG
Cuts at: 0 1785 1878
Size: 1785 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 1349 1878
Size: 1349 529
PfoI T'CCnGG_A
Cuts at: 0 1365 1859 1878
Size: 1365 494 19
Fragments arranged by size:
1365 494 19
PmlI CAC'GTG
Cuts at: 0 872 1878
Size: 872 1006
PpuMI rG'GwC_Cy
Cuts at: 0 1746 1878
Size: 1746 132
PspXI vC'TCGA_Gb
Cuts at: 0 195 1878
Size: 195 1683
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 85 117 1878
Size: 85 32 1761
Fragments arranged by size:
1761 85 32
PstI C_TGCA'G
Cuts at: 0 1281 1878
Size: 1281 597
SacI G_AGCT'C
Cuts at: 0 853 1878
Size: 853 1025
SacII CC_GC'GG
Cuts at: 0 692 1878
Size: 692 1186
SapI GCTCTTCn'nnn_
Cuts at: 0 574 1661 1861 1878
Size: 574 1087 200 17
Fragments arranged by size:
1087 574 200 17
ScaI AGT'ACT
Cuts at: 0 1036 1878
Size: 1036 842
SfcI C'TryA_G
Cuts at: 0 146 1277 1878
Size: 146 1131 601
Fragments arranged by size:
1131 601 146
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 760 1878
Size: 760 1118
SfoI GGC'GCC
Cuts at: 0 272 488 1878
Size: 272 216 1390
Fragments arranged by size:
1390 272 216
SmlI C'TyrA_G
Cuts at: 0 195 211 712 1878
Size: 195 16 501 1166
Fragments arranged by size:
1166 501 195 16
StyI C'CwwG_G
Cuts at: 0 116 123 1742 1878
Size: 116 7 1619 136
Fragments arranged by size:
1619 136 116 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1245 1741 1878
Size: 1245 496 137
Fragments arranged by size:
1245 496 137
TatI w'GTAC_w
Cuts at: 0 398 1034 1878
Size: 398 636 844
Fragments arranged by size:
844 636 398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 1878
Size: 114 1764
XhoI C'TCGA_G
Cuts at: 0 195 1878
Size: 195 1683
XmnI GAAnn'nnTTC
Cuts at: 0 1361 1878
Size: 1361 517
ZraI GAC'GTC
Cuts at: 0 1018 1878
Size: 1018 860
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BstXI BstYI BtgI BtgZI
BtsI DraI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRV HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpuMI PspXI PsrI PstI SacI SacII SapI
ScaI SfcI SfiI SfoI SmlI StyI TaqII TatI
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BfrBI BmtI BsaI BsaBI BsaWI BspEI BsrGI
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII DrdI EciI EcoNI EcoRI FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PshAI PsiI PspOMI PvuI PvuII RsrII SalI SanDI
SbfI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XmaI