Restriction map for
pF1KB3294
(Linear) (Six-base) MAPSORT of: KIBB3294.seq Check: 6061 from: 1 to: 2850
KIBB3294.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1140 1644 2850
Size: 1140 504 1206
Fragments arranged by size:
1206 1140 504
AccI GT'mk_AC
Cuts at: 0 737 2850
Size: 737 2113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 2298 2850
Size: 381 1917 552
Fragments arranged by size:
1917 552 381
AflIII A'CryG_T
Cuts at: 0 1520 1811 2090 2342 2850
Size: 1520 291 279 252 508
Fragments arranged by size:
1520 508 291 279 252
AleI CACnn'nnGTG
Cuts at: 0 2215 2850
Size: 2215 635
AlwNI CAG_nnn'CTG
Cuts at: 0 153 1566 2850
Size: 153 1413 1284
Fragments arranged by size:
1413 1284 153
ApaI G_GGCC'C
Cuts at: 0 2065 2290 2850
Size: 2065 225 560
Fragments arranged by size:
2065 560 225
ApaLI G'TGCA_C
Cuts at: 0 1528 2458 2850
Size: 1528 930 392
Fragments arranged by size:
1528 930 392
ApoI r'AATT_y
Cuts at: 0 258 444 874 950 2373 2659 2850
Size: 258 186 430 76 1423 286 191
Fragments arranged by size:
1423 430 286 258 191 186 76
AvaI C'yCGr_G
Cuts at: 0 32 519 1372 1703 1800 2850
Size: 32 487 853 331 97 1050
Fragments arranged by size:
1050 853 487 331 97 32
BanI G'GyrC_C
Cuts at: 0 124 177 1138 1698 1740 2022 2850
Size: 124 53 961 560 42 282 828
Fragments arranged by size:
961 828 560 282 124 53 42
BanII G_rGCy'C
Cuts at: 0 33 1294 2065 2290 2850
Size: 33 1261 771 225 560
Fragments arranged by size:
1261 771 560 225 33
BbeI G_GCGC'C
Cuts at: 0 1702 2026 2850
Size: 1702 324 824
Fragments arranged by size:
1702 824 324
BbsI GAAGACnn'nnnn_
Cuts at: 0 387 2027 2850
Size: 387 1640 823
Fragments arranged by size:
1640 823 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1086 1120 2850
Size: 1086 34 1730
Fragments arranged by size:
1730 1086 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2535 2850
Size: 2535 315
BclI T'GATC_A
Cuts at: 0 686 2099 2505 2850
Size: 686 1413 406 345
Fragments arranged by size:
1413 686 406 345
BlpI GC'TnA_GC
Cuts at: 0 514 2850
Size: 514 2336
Bme1580I G_kGCm'C
Cuts at: 0 1532 2065 2290 2462 2850
Size: 1532 533 225 172 388
Fragments arranged by size:
1532 533 388 225 172
BmgBI CAC'GTC
Cuts at: 0 1176 1341 2345 2850
Size: 1176 165 1004 505
Fragments arranged by size:
1176 1004 505 165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 587 2850
Size: 587 2263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 244 337 1025 1603 2208 2552 2850
Size: 181 63 93 688 578 605 344 298
Fragments arranged by size:
688 605 578 344 298 181 93 63
Bpu10I CC'TnA_GC
Cuts at: 0 146 894 2850
Size: 146 748 1956
Fragments arranged by size:
1956 748 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 2755 2850
Size: 2755 95
BsaAI yAC'GTr
Cuts at: 0 1521 2850
Size: 1521 1329
BsaHI Gr'CG_yC
Cuts at: 0 199 1699 1793 2023 2032 2044 2850
Size: 199 1500 94 230 9 12 806
Fragments arranged by size:
1500 806 230 199 94 12 9
BsaWI w'CCGG_w
Cuts at: 0 657 2635 2850
Size: 657 1978 215
Fragments arranged by size:
1978 657 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2413 2443 2850
Size: 2413 30 407
Fragments arranged by size:
2413 407 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 292 1591 2482 2773 2850
Size: 49 243 1299 891 291 77
Fragments arranged by size:
1299 891 291 243 77 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 1745 2850
Size: 324 1421 1105
Fragments arranged by size:
1421 1105 324
BsiEI CG_ry'CG
Cuts at: 0 273 2850
Size: 273 2577
BsiHKAI G_wGCw'C
Cuts at: 0 1532 2138 2170 2224 2278 2462 2850
Size: 1532 606 32 54 54 184 388
Fragments arranged by size:
1532 606 388 184 54 54 32
BsmI GAATG_Cn'
Cuts at: 0 2477 2850
Size: 2477 373
Bsp1286I G_dGCh'C
Cuts at: 0 33 1294 1532 2065 2138 2170 2224 2278
Size: 33 1261 238 533 73 32 54 54
Cuts at: 2278 2290 2462 2850
Size: 12 172 388
Fragments arranged by size:
1261 533 388 238 172 73 54 54
33 32 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 333 1140 1644 2850
Size: 333 807 504 1206
Fragments arranged by size:
1206 807 504 333
BsrBI CCG'CTC
Cuts at: 0 83 1541 2015 2850
Size: 83 1458 474 835
Fragments arranged by size:
1458 835 474 83
BsrFI r'CCGG_y
Cuts at: 0 1686 1836 1968 2850
Size: 1686 150 132 882
Fragments arranged by size:
1686 882 150 132
BsrGI T'GTAC_A
Cuts at: 0 1877 2150 2850
Size: 1877 273 700
Fragments arranged by size:
1877 700 273
BssHII G'CGCG_C
Cuts at: 0 1503 1505 1607 1782 1850 1852 2850
Size: 1503 2 102 175 68 2 998
Fragments arranged by size:
1503 998 175 102 68 2 2
BssSI C'ACGA_G
Cuts at: 0 857 1573 1901 2850
Size: 857 716 328 949
Fragments arranged by size:
949 857 716 328
BstEII G'GTnAC_C
Cuts at: 0 1272 2850
Size: 1272 1578
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2130 2232 2850
Size: 2130 102 618
Fragments arranged by size:
2130 618 102
BstYI r'GATC_y
Cuts at: 0 36 2379 2674 2850
Size: 36 2343 295 176
Fragments arranged by size:
2343 295 176 36
BstZ17I GTA'TAC
Cuts at: 0 738 2850
Size: 738 2112
BtgI C'CryG_G
Cuts at: 0 1310 1325 1982 2850
Size: 1310 15 657 868
Fragments arranged by size:
1310 868 657 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1089 2088 2850
Size: 1089 999 762
Fragments arranged by size:
1089 999 762
BtsI GCAGTG_nn'
Cuts at: 0 1555 1793 2140 2518 2850
Size: 1555 238 347 378 332
Fragments arranged by size:
1555 378 347 332 238
ClaI AT'CG_AT
Cuts at: 0 269 2850
Size: 269 2581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1977 2012 2850
Size: 1977 35 838
Fragments arranged by size:
1977 838 35
DraI TTT'AAA
Cuts at: 0 2607 2850
Size: 2607 243
EaeI y'GGCC_r
Cuts at: 0 84 135 1313 1334 1985 2016 2515 2850
Size: 84 51 1178 21 651 31 499 335
Fragments arranged by size:
1178 651 499 335 84 51 31 21
EarI CTCTTCn'nnn_
Cuts at: 0 503 2850
Size: 503 2347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 184 2850
Size: 184 2666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 244 337 381 1025 1603 2208 2298
Size: 181 63 93 44 644 578 605 90
Cuts at: 2298 2552 2850
Size: 254 298
Fragments arranged by size:
644 605 578 298 254 181 93 90
63 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 1912 2467 2850
Size: 223 1689 555 383
Fragments arranged by size:
1689 555 383 223
EcoO109I rG'GnC_Cy
Cuts at: 0 21 150 216 2286 2500 2850
Size: 21 129 66 2070 214 350
Fragments arranged by size:
2070 350 214 129 66 21
EcoRI G'AATT_C
Cuts at: 0 258 444 2850
Size: 258 186 2406
Fragments arranged by size:
2406 258 186
HaeII r_GCGC'y
Cuts at: 0 1235 1693 1702 1975 2026 2077 2161 2850
Size: 1235 458 9 273 51 51 84 689
Fragments arranged by size:
1235 689 458 273 84 51 51 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 877 909 978 1010 1117 1149
Size: 517 32 328 32 69 32 107 32
Cuts at: 1149 1237 1269 1276 1308 1783 1815 2850
Size: 88 32 7 32 475 32 1035
Fragments arranged by size:
1035 517 475 328 107 88 69 32
32 32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 0 2088 2586 2850
Size: 2088 498 264
Fragments arranged by size:
2088 498 264
KasI G'GCGC_C
Cuts at: 0 1698 2022 2850
Size: 1698 324 828
Fragments arranged by size:
1698 828 324
MluI A'CGCG_T
Cuts at: 0 1811 2850
Size: 1811 1039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1205 2850
Size: 1205 1645
MscI TGG'CCA
Cuts at: 0 2517 2850
Size: 2517 333
MslI CAynn'nnrTG
Cuts at: 0 1117 2215 2850
Size: 1117 1098 635
Fragments arranged by size:
1117 1098 635
MspA1I CmG'CkG
Cuts at: 0 339 2597 2850
Size: 339 2258 253
Fragments arranged by size:
2258 339 253
NaeI GCC'GGC
Cuts at: 0 1688 1838 1970 2850
Size: 1688 150 132 880
Fragments arranged by size:
1688 880 150 132
NarI GG'CG_CC
Cuts at: 0 1699 2023 2850
Size: 1699 324 827
Fragments arranged by size:
1699 827 324
NgoMIV G'CCGG_C
Cuts at: 0 1686 1836 1968 2850
Size: 1686 150 132 882
Fragments arranged by size:
1686 882 150 132
NruI TCG'CGA
Cuts at: 0 275 1244 2850
Size: 275 969 1606
Fragments arranged by size:
1606 969 275
NspI r_CATG'y
Cuts at: 0 2448 2850
Size: 2448 402
PfoI T'CCnGG_A
Cuts at: 0 104 1907 2493 2850
Size: 104 1803 586 357
Fragments arranged by size:
1803 586 357 104
PpuMI rG'GwC_Cy
Cuts at: 0 150 216 2850
Size: 150 66 2634
Fragments arranged by size:
2634 150 66
PshAI GACnn'nnGTC
Cuts at: 0 1903 2850
Size: 1903 947
PspOMI G'GGCC_C
Cuts at: 0 2061 2286 2850
Size: 2061 225 564
Fragments arranged by size:
2061 564 225
PspXI vC'TCGA_Gb
Cuts at: 0 1703 2850
Size: 1703 1147
PstI C_TGCA'G
Cuts at: 0 239 344 1562 1595 1655 1835 2850
Size: 239 105 1218 33 60 180 1015
Fragments arranged by size:
1218 1015 239 180 105 60 33
PvuI CG_AT'CG
Cuts at: 0 273 2850
Size: 273 2577
PvuII CAG'CTG
Cuts at: 0 2597 2850
Size: 2597 253
SapI GCTCTTCn'nnn_
Cuts at: 0 503 2850
Size: 503 2347
SfcI C'TryA_G
Cuts at: 0 235 340 761 1558 1591 1651 1831 2850
Size: 235 105 421 797 33 60 180 1019
Fragments arranged by size:
1019 797 421 235 180 105 60 33
SfoI GGC'GCC
Cuts at: 0 1700 2024 2850
Size: 1700 324 826
Fragments arranged by size:
1700 826 324
SgrAI Cr'CCGG_yG
Cuts at: 0 1686 2850
Size: 1686 1164
SmaI CCC'GGG
Cuts at: 0 34 2850
Size: 34 2816
SmlI C'TyrA_G
Cuts at: 0 1703 2850
Size: 1703 1147
SphI G_CATG'C
Cuts at: 0 2448 2850
Size: 2448 402
SspI AAT'ATT
Cuts at: 0 419 595 2850
Size: 419 176 2255
Fragments arranged by size:
2255 419 176
StuI AGG'CCT
Cuts at: 0 2324 2850
Size: 2324 526
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1245 1699 1744 2041 2307 2314 2850
Size: 1245 454 45 297 266 7 536
Fragments arranged by size:
1245 536 454 297 266 45 7
TatI w'GTAC_w
Cuts at: 0 1376 1877 2150 2850
Size: 1376 501 273 700
Fragments arranged by size:
1376 700 501 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 764 796 2850
Size: 764 32 2054
Fragments arranged by size:
2054 764 32
XbaI T'CTAG_A
Cuts at: 0 433 2850
Size: 433 2417
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1323 2850
Size: 1323 1527
XhoI C'TCGA_G
Cuts at: 0 1703 2850
Size: 1703 1147
XmaI C'CCGG_G
Cuts at: 0 32 2850
Size: 32 2818
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BcgI BciVI
BclI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NruI NspI PfoI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII SapI
SfcI SfoI SgrAI SmaI SmlI SphI SspI StuI
TaqII TatI TstI XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BfrBI BglI BglII BmrI BmtI BpuEI BsaBI BseYI
BsiWI BsmBI BspEI BspHI BsrDI BstAPI BstBI Bsu36I
DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI NcoI NdeI NheI
NotI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PsiI PsrI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SrfI
StyI SwaI Tth111I XmnI ZraI