Restriction map for
pF1KB3289
(Linear) (Six-base) MAPSORT of: KIBB3289.seq Check: 1313 from: 1 to: 2409
KIBB3289.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 765 1098 1117 1182 1369 1518 1602
Size: 449 316 333 19 65 187 149 84
Cuts at: 1602 1705 1854 2106 2190 2274 2409
Size: 103 149 252 84 84 135
Fragments arranged by size:
449 333 316 252 187 149 149 135
103 84 84 84 65 19
AflII C'TTAA_G
Cuts at: 0 919 2409
Size: 919 1490
AflIII A'CryG_T
Cuts at: 0 841 903 2409
Size: 841 62 1506
Fragments arranged by size:
1506 841 62
AleI CACnn'nnGTG
Cuts at: 0 656 2409
Size: 656 1753
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 335 2409
Size: 335 2074
ApaI G_GGCC'C
Cuts at: 0 79 2409
Size: 79 2330
ApaLI G'TGCA_C
Cuts at: 0 1964 2074 2409
Size: 1964 110 335
Fragments arranged by size:
1964 335 110
ApoI r'AATT_y
Cuts at: 0 228 422 566 943 1206 1542 1808 2409
Size: 228 194 144 377 263 336 266 601
Fragments arranged by size:
601 377 336 266 263 228 194 144
BamHI G'GATC_C
Cuts at: 0 1820 2409
Size: 1820 589
BanII G_rGCy'C
Cuts at: 0 79 1714 2409
Size: 79 1635 695
Fragments arranged by size:
1635 695 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 225 1375 1860 2131 2196 2207 2409
Size: 225 1150 485 271 65 11 202
Fragments arranged by size:
1150 485 271 225 202 65 11
BclI T'GATC_A
Cuts at: 0 353 717 2409
Size: 353 364 1692
Fragments arranged by size:
1692 364 353
BfrBI ATG'CAT
Cuts at: 0 1183 2064 2409
Size: 1183 881 345
Fragments arranged by size:
1183 881 345
BglII A'GATC_T
Cuts at: 0 1800 2409
Size: 1800 609
Bme1580I G_kGCm'C
Cuts at: 0 79 1968 2078 2409
Size: 79 1889 110 331
Fragments arranged by size:
1889 331 110 79
BmgBI CAC'GTC
Cuts at: 0 36 2409
Size: 36 2373
BmrI ACTGGGnnnn_n'
Cuts at: 0 485 871 1166 2258 2409
Size: 485 386 295 1092 151
Fragments arranged by size:
1092 485 386 295 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 1430 1514 2018 2409
Size: 777 653 84 504 391
Fragments arranged by size:
777 653 504 391 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 2118 2409
Size: 519 1599 291
Fragments arranged by size:
1599 519 291
BsaAI yAC'GTr
Cuts at: 0 987 2409
Size: 987 1422
BsaBI GATnn'nnATC
Cuts at: 0 806 2409
Size: 806 1603
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 1111 1279 1699 1867 2409
Size: 70 1041 168 420 168 542
Fragments arranged by size:
1041 542 420 168 168 70
BseYI C'CCAG_C
Cuts at: 0 72 2409
Size: 72 2337
BsiEI CG_ry'CG
Cuts at: 0 1959 2409
Size: 1959 450
BsiHKAI G_wGCw'C
Cuts at: 0 1714 1968 2078 2409
Size: 1714 254 110 331
Fragments arranged by size:
1714 331 254 110
Bsp1286I G_dGCh'C
Cuts at: 0 79 1714 1968 2078 2409
Size: 79 1635 254 110 331
Fragments arranged by size:
1635 331 254 110 79
BspHI T'CATG_A
Cuts at: 0 633 2409
Size: 633 1776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 2409
Size: 137 2272
BsrDI GCAATG_nn'
Cuts at: 0 1021 1056 1521 2409
Size: 1021 35 465 888
Fragments arranged by size:
1021 888 465 35
BstYI r'GATC_y
Cuts at: 0 1800 1820 2409
Size: 1800 20 589
Fragments arranged by size:
1800 589 20
BtsI GCAGTG_nn'
Cuts at: 0 26 2409
Size: 26 2383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1503 1538 2409
Size: 1503 35 871
Fragments arranged by size:
1503 871 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 411 2409
Size: 411 1998
EarI CTCTTCn'nnn_
Cuts at: 0 281 1522 1763 1942 2409
Size: 281 1241 241 179 467
Fragments arranged by size:
1241 467 281 241 179
EcoICRI GAG'CTC
Cuts at: 0 1712 2409
Size: 1712 697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 765 777 1098 1117 1182 1369 1430
Size: 449 316 12 321 19 65 187 61
Cuts at: 1430 1514 1518 1602 1705 1854 2018 2106 2190
Size: 84 4 84 103 149 164 88 84
Cuts at: 2190 2274 2409
Size: 84 135
Fragments arranged by size:
449 321 316 187 164 149 135 103
88 84 84 84 84 65 61 19
12 4
EcoO109I rG'GnC_Cy
Cuts at: 0 76 1109 2409
Size: 76 1033 1300
Fragments arranged by size:
1300 1033 76
EcoRI G'AATT_C
Cuts at: 0 943 1206 1542 2409
Size: 943 263 336 867
Fragments arranged by size:
943 867 336 263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 307 2064 2409
Size: 307 1757 345
Fragments arranged by size:
1757 345 307
HaeII r_GCGC'y
Cuts at: 0 959 2409
Size: 959 1450
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 262 294 615 647 702 734 1185 1217
Size: 262 32 321 32 55 32 451 32
Cuts at: 1217 1449 1481 1941 1953 1973 1985 2409
Size: 232 32 460 12 20 12 424
Fragments arranged by size:
460 451 424 321 262 232 55 32
32 32 32 32 20 12 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1183 1939 2409
Size: 601 582 756 470
Fragments arranged by size:
756 601 582 470
MslI CAynn'nnrTG
Cuts at: 0 635 656 1005 1173 1257 1425 1509 1745
Size: 635 21 349 168 84 168 84 236
Cuts at: 1745 1761 1929 2013 2097 2153 2181 2333 2409
Size: 16 168 84 84 56 28 152 76
Fragments arranged by size:
635 349 236 168 168 168 152 84
84 84 84 76 56 28 21 16
MspA1I CmG'CkG
Cuts at: 0 1734 2409
Size: 1734 675
NdeI CA'TA_TG
Cuts at: 0 1426 2409
Size: 1426 983
NsiI A_TGCA'T
Cuts at: 0 1185 2066 2409
Size: 1185 881 343
Fragments arranged by size:
1185 881 343
NspI r_CATG'y
Cuts at: 0 845 907 1187 2064 2409
Size: 845 62 280 877 345
Fragments arranged by size:
877 845 345 280 62
PciI A'CATG_T
Cuts at: 0 841 903 2409
Size: 841 62 1506
Fragments arranged by size:
1506 841 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 620 2409
Size: 620 1789
PmlI CAC'GTG
Cuts at: 0 987 2409
Size: 987 1422
PsiI TTA'TAA
Cuts at: 0 1088 2264 2409
Size: 1088 1176 145
Fragments arranged by size:
1176 1088 145
PspOMI G'GGCC_C
Cuts at: 0 75 2409
Size: 75 2334
PvuII CAG'CTG
Cuts at: 0 1734 2409
Size: 1734 675
SacI G_AGCT'C
Cuts at: 0 1714 2409
Size: 1714 695
SapI GCTCTTCn'nnn_
Cuts at: 0 281 1522 2409
Size: 281 1241 887
Fragments arranged by size:
1241 887 281
ScaI AGT'ACT
Cuts at: 0 2119 2409
Size: 2119 290
SexAI A'CCwGG_T
Cuts at: 0 334 395 2409
Size: 334 61 2014
Fragments arranged by size:
2014 334 61
SfcI C'TryA_G
Cuts at: 0 173 964 1815 2224 2409
Size: 173 791 851 409 185
Fragments arranged by size:
851 791 409 185 173
SmlI C'TyrA_G
Cuts at: 0 498 919 2133 2409
Size: 498 421 1214 276
Fragments arranged by size:
1214 498 421 276
StuI AGG'CCT
Cuts at: 0 1123 1879 2131 2409
Size: 1123 756 252 278
Fragments arranged by size:
1123 756 278 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1945 2201 2409
Size: 1945 256 208
Fragments arranged by size:
1945 256 208
TatI w'GTAC_w
Cuts at: 0 648 900 1429 2117 2409
Size: 648 252 529 688 292
Fragments arranged by size:
688 648 529 292 252
Tth111I GACn'n_nGTC
Cuts at: 0 537 2409
Size: 537 1872
XmnI GAAnn'nnTTC
Cuts at: 0 7 526 1027 1168 1531 1615 1867 1951
Size: 7 519 501 141 363 84 252 84
Cuts at: 1951 2035 2409
Size: 84 374
Fragments arranged by size:
519 501 374 363 252 141 84 84
84 7
Enzymes that do cut:
AcuI AflII AflIII AleI AlfI ApaI ApaLI ApoI
BamHI BanII BbsI BclI BfrBI BglII Bme1580I BmgBI
BmrI BpmI BpuEI BsaAI BsaBI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BstYI BtsI CspCI
DrdI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I MmeI MslI MspA1I NdeI NsiI NspI PciI
PflMI PmlI PsiI PspOMI PvuII SacI SapI ScaI
SexAI SfcI SmlI StuI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AloI AlwNI AscI AseI AsiSI AvaI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BglI BlpI BmtI
BplI Bpu10I BsaI BsaHI BsaWI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII EaeI EagI EciI EcoNI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PfoI PmeI PpiI PpuMI
PshAI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TstI XbaI
XcmI XhoI XmaI ZraI