Restriction map for
pF1KB3283
(Linear) (Six-base) MAPSORT of: KIBB3283.seq Check: 711 from: 1 to: 3567
KIBB3283.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 490 716 1679 3500 3567
Size: 490 226 963 1821 67
Fragments arranged by size:
1821 963 490 226 67
AccI GT'mk_AC
Cuts at: 0 560 3567
Size: 560 3007
Acc65I G'GTAC_C
Cuts at: 0 908 3567
Size: 908 2659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 579 1156 1429 3567
Size: 43 536 577 273 2138
Fragments arranged by size:
2138 577 536 273 43
AflIII A'CryG_T
Cuts at: 0 3164 3567
Size: 3164 403
AleI CACnn'nnGTG
Cuts at: 0 936 3567
Size: 936 2631
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2044 3067 3567
Size: 2044 1023 500
Fragments arranged by size:
2044 1023 500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2408 2440 3567
Size: 2408 32 1127
Fragments arranged by size:
2408 1127 32
AlwNI CAG_nnn'CTG
Cuts at: 0 817 1109 1312 1891 2700 3387 3567
Size: 817 292 203 579 809 687 180
Fragments arranged by size:
817 809 687 579 292 203 180
ApaI G_GGCC'C
Cuts at: 0 205 241 1230 1740 2100 2255 3567
Size: 205 36 989 510 360 155 1312
Fragments arranged by size:
1312 989 510 360 205 155 36
ApaLI G'TGCA_C
Cuts at: 0 1941 3567
Size: 1941 1626
AvaI C'yCGr_G
Cuts at: 0 95 1249 1380 1437 2087 2101 2463 3567
Size: 95 1154 131 57 650 14 362 1104
Fragments arranged by size:
1154 1104 650 362 131 95 57 14
BamHI G'GATC_C
Cuts at: 0 627 2627 3567
Size: 627 2000 940
Fragments arranged by size:
2000 940 627
BanI G'GyrC_C
Cuts at: 0 28 474 704 908 938 1051 3080 3232
Size: 28 446 230 204 30 113 2029 152
Cuts at: 3232 3247 3287 3354 3567
Size: 15 40 67 213
Fragments arranged by size:
2029 446 230 213 204 152 113 67
40 30 28 15
BanII G_rGCy'C
Cuts at: 0 83 205 241 465 659 1230 1740 2100
Size: 83 122 36 224 194 571 510 360
Cuts at: 2100 2255 2552 2707 2800 2957 2986 3128 3369
Size: 155 297 155 93 157 29 142 241
Cuts at: 3369 3567
Size: 198
Fragments arranged by size:
571 510 360 297 241 224 198 194
157 155 155 142 122 93 83 36
29
BbeI G_GCGC'C
Cuts at: 0 3236 3251 3291 3567
Size: 3236 15 40 276
Fragments arranged by size:
3236 276 40 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 52 1147 1595 3567
Size: 52 1095 448 1972
Fragments arranged by size:
1972 1095 448 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1641 1675 3567
Size: 1641 34 1892
Fragments arranged by size:
1892 1641 34
BglI GCCn_nnn'nGGC
Cuts at: 0 724 1037 3078 3567
Size: 724 313 2041 489
Fragments arranged by size:
2041 724 489 313
BlpI GC'TnA_GC
Cuts at: 0 1648 2017 2701 3449 3567
Size: 1648 369 684 748 118
Fragments arranged by size:
1648 748 684 369 118
Bme1580I G_kGCm'C
Cuts at: 0 31 205 241 346 479 587 707 824
Size: 31 174 36 105 133 108 120 117
Cuts at: 824 943 1230 1503 1529 1740 1945 1961 2066
Size: 119 287 273 26 211 205 16 105
Cuts at: 2066 2100 2255 2679 3090 3177 3220 3567
Size: 34 155 424 411 87 43 347
Fragments arranged by size:
424 411 347 287 273 211 205 174
155 133 120 119 117 108 105 105
87 43 36 34 31 26 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1901 1980 2357 3522 3567
Size: 1901 79 377 1165 45
Fragments arranged by size:
1901 1165 377 79 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1577 2778 3567
Size: 1577 1201 789
Fragments arranged by size:
1577 1201 789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 475 1063 1414 1532 1590 1980 2483
Size: 460 15 588 351 118 58 390 503
Cuts at: 2483 2616 2971 3190 3259 3313 3567
Size: 133 355 219 69 54 254
Fragments arranged by size:
588 503 460 390 355 351 254 219
133 118 69 58 54 15
Bpu10I CC'TnA_GC
Cuts at: 0 160 371 600 950 2771 3567
Size: 160 211 229 350 1821 796
Fragments arranged by size:
1821 796 350 229 211 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 3567
Size: 376 3191
BsaI GGTCTCn'nnnn_
Cuts at: 0 436 2197 2215 3137 3424 3567
Size: 436 1761 18 922 287 143
Fragments arranged by size:
1761 922 436 287 143 18
BsaAI yAC'GTr
Cuts at: 0 3165 3567
Size: 3165 402
BsaHI Gr'CG_yC
Cuts at: 0 155 3182 3233 3248 3288 3567
Size: 155 3027 51 15 40 279
Fragments arranged by size:
3027 279 155 51 40 15
BsaWI w'CCGG_w
Cuts at: 0 1293 2877 3567
Size: 1293 1584 690
Fragments arranged by size:
1584 1293 690
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2675 2705 3332 3362 3406 3436 3567
Size: 2675 30 627 30 44 30 131
Fragments arranged by size:
2675 627 131 44 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1216 1535 1951 2215 3313 3567
Size: 1216 319 416 264 1098 254
Fragments arranged by size:
1216 1098 416 319 264 254
BseYI C'CCAG_C
Cuts at: 0 13 270 883 915 965 1072 1117 1327
Size: 13 257 613 32 50 107 45 210
Cuts at: 1327 1739 2226 2552 2720 2725 2957 3185 3469
Size: 412 487 326 168 5 232 228 284
Cuts at: 3469 3567
Size: 98
Fragments arranged by size:
613 487 412 326 284 257 232 228
210 168 107 98 50 45 32 13
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 727 2713 2797 3400 3567
Size: 91 636 1986 84 603 167
Fragments arranged by size:
1986 636 603 167 91 84
BsiEI CG_ry'CG
Cuts at: 0 3224 3567
Size: 3224 343
BsiHKAI G_wGCw'C
Cuts at: 0 166 465 1945 2023 2594 3397 3567
Size: 166 299 1480 78 571 803 170
Fragments arranged by size:
1480 803 571 299 170 166 78
BsmI GAATG_Cn'
Cuts at: 0 325 635 1784 3567
Size: 325 310 1149 1783
Fragments arranged by size:
1783 1149 325 310
Bsp1286I G_dGCh'C
Cuts at: 0 31 83 166 205 241 346 465 479
Size: 31 52 83 39 36 105 119 14
Cuts at: 479 587 659 707 824 943 1230 1503 1529
Size: 108 72 48 117 119 287 273 26
Cuts at: 1529 1740 1945 1961 2023 2066 2100 2255 2552
Size: 211 205 16 62 43 34 155 297
Cuts at: 2552 2594 2679 2707 2800 2957 2986 3090 3128
Size: 42 85 28 93 157 29 104 38
Cuts at: 3128 3177 3220 3369 3397 3567
Size: 49 43 149 28 170
Fragments arranged by size:
297 287 273 211 205 170 157 155
149 119 119 117 108 105 104 93
85 83 72 62 52 49 48 43
43 42 39 38 36 34 31 29
28 28 26 16 14
BspEI T'CCGG_A
Cuts at: 0 2877 3567
Size: 2877 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 490 716 1679 3500 3567
Size: 490 226 963 1821 67
Fragments arranged by size:
1821 963 490 226 67
BsrBI CCG'CTC
Cuts at: 0 795 1719 3567
Size: 795 924 1848
Fragments arranged by size:
1848 924 795
BsrDI GCAATG_nn'
Cuts at: 0 1271 2033 2172 2638 3567
Size: 1271 762 139 466 929
Fragments arranged by size:
1271 929 762 466 139
BsrFI r'CCGG_y
Cuts at: 0 773 1209 1643 1856 3567
Size: 773 436 434 213 1711
Fragments arranged by size:
1711 773 436 434 213
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1344 1805 3567
Size: 1344 461 1762
Fragments arranged by size:
1762 1344 461
BstXI CCAn_nnnn'nTGG
Cuts at: 0 176 453 533 754 1510 2388 2733 2847
Size: 176 277 80 221 756 878 345 114
Cuts at: 2847 3567
Size: 720
Fragments arranged by size:
878 756 720 345 277 221 176 114
80
BstYI r'GATC_y
Cuts at: 0 449 627 1008 2423 2627 3567
Size: 449 178 381 1415 204 940
Fragments arranged by size:
1415 940 449 381 204 178
BstZ17I GTA'TAC
Cuts at: 0 561 3567
Size: 561 3006
Bsu36I CC'TnA_GG
Cuts at: 0 1193 3567
Size: 1193 2374
BtgI C'CryG_G
Cuts at: 0 111 1812 1931 2294 2895 3542 3567
Size: 111 1701 119 363 601 647 25
Fragments arranged by size:
1701 647 601 363 119 111 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 908 1851 3567
Size: 908 943 1716
Fragments arranged by size:
1716 943 908
BtsI GCAGTG_nn'
Cuts at: 0 423 739 1310 2021 2064 3067 3536 3567
Size: 423 316 571 711 43 1003 469 31
Fragments arranged by size:
1003 711 571 469 423 316 43 31
ClaI AT'CG_AT
Cuts at: 0 1357 3567
Size: 1357 2210
DraIII CAC_nnn'GTG
Cuts at: 0 3158 3378 3567
Size: 3158 220 189
Fragments arranged by size:
3158 220 189
EaeI y'GGCC_r
Cuts at: 0 384 771 1288 1416 2244 3029 3221 3539
Size: 384 387 517 128 828 785 192 318
Cuts at: 3539 3567
Size: 28
Fragments arranged by size:
828 785 517 387 384 318 192 128
28
EagI C'GGCC_G
Cuts at: 0 3221 3567
Size: 3221 346
EarI CTCTTCn'nnn_
Cuts at: 0 802 1825 2587 3567
Size: 802 1023 762 980
Fragments arranged by size:
1023 980 802 762
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3184 3567
Size: 3184 383
EcoICRI GAG'CTC
Cuts at: 0 463 3567
Size: 463 3104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 460 475 579 1063 1156 1414 1429
Size: 43 417 15 104 484 93 258 15
Cuts at: 1429 1532 1590 1980 2483 2616 2971 3190 3259
Size: 103 58 390 503 133 355 219 69
Cuts at: 3259 3313 3567
Size: 54 254
Fragments arranged by size:
503 484 417 390 355 258 254 219
133 104 103 93 69 58 54 43
15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 147 1304 1565 2522 2584 3295 3328 3567
Size: 147 1157 261 957 62 711 33 239
Fragments arranged by size:
1157 957 711 261 239 147 62 33
EcoO109I rG'GnC_Cy
Cuts at: 0 201 202 237 238 1038 1106 1227 1405
Size: 201 1 35 1 800 68 121 178
Cuts at: 1405 1434 1725 2097 2251 2252 2478 3333 3384
Size: 29 291 372 154 1 226 855 51
Cuts at: 3384 3466 3567
Size: 82 101
Fragments arranged by size:
855 800 372 291 226 201 178 154
121 101 82 68 51 35 29 1
1 1
EcoRV GAT'ATC
Cuts at: 0 2055 3567
Size: 2055 1512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 675 3118 3567
Size: 675 2443 449
Fragments arranged by size:
2443 675 449
FspI TGC'GCA
Cuts at: 0 2473 3567
Size: 2473 1094
HaeII r_GCGC'y
Cuts at: 0 2701 3236 3251 3291 3567
Size: 2701 535 15 40 276
Fragments arranged by size:
2701 535 276 40 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1363 1395 2652 2684 2782 2814 3567
Size: 1363 32 1257 32 98 32 753
Fragments arranged by size:
1363 1257 753 98 32 32 32
HincII GTy'rAC
Cuts at: 0 264 3567
Size: 264 3303
KasI G'GCGC_C
Cuts at: 0 3232 3247 3287 3567
Size: 3232 15 40 280
Fragments arranged by size:
3232 280 40 15
KpnI G_GTAC'C
Cuts at: 0 912 3567
Size: 912 2655
MscI TGG'CCA
Cuts at: 0 386 1418 3567
Size: 386 1032 2149
Fragments arranged by size:
2149 1032 386
MslI CAynn'nnrTG
Cuts at: 0 380 527 936 2026 3049 3567
Size: 380 147 409 1090 1023 518
Fragments arranged by size:
1090 1023 518 409 380 147
MspA1I CmG'CkG
Cuts at: 0 120 321 667 915 969 1510 1743 1847
Size: 120 201 346 248 54 541 233 104
Cuts at: 1847 1933 2218 2296 3260 3567
Size: 86 285 78 964 307
Fragments arranged by size:
964 541 346 307 285 248 233 201
120 104 86 78 54
NaeI GCC'GGC
Cuts at: 0 775 1645 3567
Size: 775 870 1922
Fragments arranged by size:
1922 870 775
NarI GG'CG_CC
Cuts at: 0 3233 3248 3288 3567
Size: 3233 15 40 279
Fragments arranged by size:
3233 279 40 15
NcoI C'CATG_G
Cuts at: 0 111 1812 2895 3567
Size: 111 1701 1083 672
Fragments arranged by size:
1701 1083 672 111
NgoMIV G'CCGG_C
Cuts at: 0 773 1643 3567
Size: 773 870 1924
Fragments arranged by size:
1924 870 773
NspI r_CATG'y
Cuts at: 0 216 3058 3567
Size: 216 2842 509
Fragments arranged by size:
2842 509 216
PasI CC'CwG_GG
Cuts at: 0 198 554 1103 1624 2439 2524 3567
Size: 198 356 549 521 815 85 1043
Fragments arranged by size:
1043 815 549 521 356 198 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 895 2007 3567
Size: 321 574 1112 1560
Fragments arranged by size:
1560 1112 574 321
PfoI T'CCnGG_A
Cuts at: 0 452 1430 2387 2426 2585 3567
Size: 452 978 957 39 159 982
Fragments arranged by size:
982 978 957 452 159 39
PmlI CAC'GTG
Cuts at: 0 3165 3567
Size: 3165 402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 3567
Size: 1078 32 2457
Fragments arranged by size:
2457 1078 32
PpuMI rG'GwC_Cy
Cuts at: 0 1405 1434 3466 3567
Size: 1405 29 2032 101
Fragments arranged by size:
2032 1405 101 29
PspOMI G'GGCC_C
Cuts at: 0 201 237 1226 1736 2096 2251 3567
Size: 201 36 989 510 360 155 1316
Fragments arranged by size:
1316 989 510 360 201 155 36
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 892 924 3567
Size: 892 32 2643
Fragments arranged by size:
2643 892 32
PstI C_TGCA'G
Cuts at: 0 713 1317 1580 1801 1852 2546 3363 3567
Size: 713 604 263 221 51 694 817 204
Fragments arranged by size:
817 713 694 604 263 221 204 51
PvuII CAG'CTG
Cuts at: 0 667 915 1510 3260 3567
Size: 667 248 595 1750 307
Fragments arranged by size:
1750 667 595 307 248
SacI G_AGCT'C
Cuts at: 0 465 3567
Size: 465 3102
SacII CC_GC'GG
Cuts at: 0 1934 2297 3567
Size: 1934 363 1270
Fragments arranged by size:
1934 1270 363
SanDI GG'GwC_CC
Cuts at: 0 3466 3567
Size: 3466 101
SapI GCTCTTCn'nnn_
Cuts at: 0 802 3567
Size: 802 2765
SbfI CC_TGCA'GG
Cuts at: 0 3363 3567
Size: 3363 204
ScaI AGT'ACT
Cuts at: 0 2884 3567
Size: 2884 683
SexAI A'CCwGG_T
Cuts at: 0 2033 3035 3374 3567
Size: 2033 1002 339 193
Fragments arranged by size:
2033 1002 339 193
SfcI C'TryA_G
Cuts at: 0 709 1313 1576 1797 1848 2542 3359 3567
Size: 709 604 263 221 51 694 817 208
Fragments arranged by size:
817 709 694 604 263 221 208 51
SfoI GGC'GCC
Cuts at: 0 3234 3249 3289 3567
Size: 3234 15 40 278
Fragments arranged by size:
3234 278 40 15
SmaI CCC'GGG
Cuts at: 0 1439 2089 2103 2465 3567
Size: 1439 650 14 362 1102
Fragments arranged by size:
1439 1102 650 362 14
SmlI C'TyrA_G
Cuts at: 0 95 391 3567
Size: 95 296 3176
Fragments arranged by size:
3176 296 95
SphI G_CATG'C
Cuts at: 0 3058 3567
Size: 3058 509
StuI AGG'CCT
Cuts at: 0 854 1093 1658 3110 3567
Size: 854 239 565 1452 457
Fragments arranged by size:
1452 854 565 457 239
StyI C'CwwG_G
Cuts at: 0 111 206 659 903 934 1752 1812 2895
Size: 111 95 453 244 31 818 60 1083
Cuts at: 2895 3567
Size: 672
Fragments arranged by size:
1083 818 672 453 244 111 95 60
31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 462 979 1008 1706 1843 2191 3567
Size: 462 517 29 698 137 348 1376
Fragments arranged by size:
1376 698 517 462 348 137 29
TatI w'GTAC_w
Cuts at: 0 2882 3567
Size: 2882 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1681 1713 2675 2707 3332 3364 3406 3438
Size: 1681 32 962 32 625 32 42 32
Cuts at: 3438 3567
Size: 129
Fragments arranged by size:
1681 962 625 129 42 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3037 3567
Size: 3037 530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2004 3074 3567
Size: 2004 1070 493
Fragments arranged by size:
2004 1070 493
XhoI C'TCGA_G
Cuts at: 0 95 3567
Size: 95 3472
XmaI C'CCGG_G
Cuts at: 0 1437 2087 2101 2463 3567
Size: 1437 650 14 362 1104
Fragments arranged by size:
1437 1104 650 362 14
XmnI GAAnn'nnTTC
Cuts at: 0 3097 3567
Size: 3097 470
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI
BbsI BcgI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PsrI PstI PvuII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI ApoI AscI
AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI
BglII BmgBI BmtI BsaBI BsiWI BsmBI BspHI BsrGI
BssHII BssSI BstBI BstEII CspCI DraI DrdI EcoRI
FseI FspAI HindIII HpaI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PshAI
PsiI PspXI PvuI RsrII SalI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XbaI ZraI