Restriction map for
pF1KB3281
(Linear) (Six-base) MAPSORT of: KIBB3281.seq Check: 2654 from: 1 to: 2361
KIBB3281.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 612 2361
Size: 612 1749
AccI GT'mk_AC
Cuts at: 0 2033 2361
Size: 2033 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1045 1230 2361
Size: 268 777 185 1131
Fragments arranged by size:
1131 777 268 185
AflIII A'CryG_T
Cuts at: 0 287 2003 2222 2361
Size: 287 1716 219 139
Fragments arranged by size:
1716 287 219 139
AleI CACnn'nnGTG
Cuts at: 0 1518 2361
Size: 1518 843
AlwNI CAG_nnn'CTG
Cuts at: 0 180 201 663 717 780 906 1023 1051
Size: 180 21 462 54 63 126 117 28
Cuts at: 1051 1471 1608 2361
Size: 420 137 753
Fragments arranged by size:
753 462 420 180 137 126 117 63
54 28 21
ApaI G_GGCC'C
Cuts at: 0 800 2361
Size: 800 1561
ApaLI G'TGCA_C
Cuts at: 0 1084 2361
Size: 1084 1277
ApoI r'AATT_y
Cuts at: 0 1315 1540 1683 2361
Size: 1315 225 143 678
Fragments arranged by size:
1315 678 225 143
AvaI C'yCGr_G
Cuts at: 0 802 2361
Size: 802 1559
BamHI G'GATC_C
Cuts at: 0 542 2342 2361
Size: 542 1800 19
Fragments arranged by size:
1800 542 19
BanI G'GyrC_C
Cuts at: 0 1228 1479 2361
Size: 1228 251 882
Fragments arranged by size:
1228 882 251
BanII G_rGCy'C
Cuts at: 0 580 800 2315 2361
Size: 580 220 1515 46
Fragments arranged by size:
1515 580 220 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 1033 2361
Size: 709 324 1328
Fragments arranged by size:
1328 709 324
BciVI GTATCCnnnnn_n'
Cuts at: 0 5 2086 2361
Size: 5 2081 275
Fragments arranged by size:
2081 275 5
BglII A'GATC_T
Cuts at: 0 138 1184 2361
Size: 138 1046 1177
Fragments arranged by size:
1177 1046 138
BlpI GC'TnA_GC
Cuts at: 0 20 574 887 2361
Size: 20 554 313 1474
Fragments arranged by size:
1474 554 313 20
Bme1580I G_kGCm'C
Cuts at: 0 266 800 1088 1242 2361
Size: 266 534 288 154 1119
Fragments arranged by size:
1119 534 288 266 154
BmgBI CAC'GTC
Cuts at: 0 819 2361
Size: 819 1542
BmrI ACTGGGnnnn_n'
Cuts at: 0 99 916 1332 1662 2361
Size: 99 817 416 330 699
Fragments arranged by size:
817 699 416 330 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 265 368 409 595 652 1654 2296
Size: 202 63 103 41 186 57 1002 642
Cuts at: 2296 2361
Size: 65
Fragments arranged by size:
1002 642 202 186 103 65 63 57
41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1738 2361
Size: 1132 606 623
Fragments arranged by size:
1132 623 606
BsaAI yAC'GTr
Cuts at: 0 290 2004 2097 2171 2361
Size: 290 1714 93 74 190
Fragments arranged by size:
1714 290 190 93 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 625 655 2361
Size: 625 30 1706
Fragments arranged by size:
1706 625 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 943 1004 2323 2361
Size: 460 483 61 1319 38
Fragments arranged by size:
1319 483 460 61 38
BseYI C'CCAG_C
Cuts at: 0 173 258 569 2193 2361
Size: 173 85 311 1624 168
Fragments arranged by size:
1624 311 173 168 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1576 2361
Size: 214 1362 785
Fragments arranged by size:
1362 785 214
BsiEI CG_ry'CG
Cuts at: 0 1974 2361
Size: 1974 387
BsiHKAI G_wGCw'C
Cuts at: 0 221 893 1088 2315 2361
Size: 221 672 195 1227 46
Fragments arranged by size:
1227 672 221 195 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 595 2361
Size: 595 1766
Bsp1286I G_dGCh'C
Cuts at: 0 221 266 580 800 893 1088 1242 2315
Size: 221 45 314 220 93 195 154 1073
Cuts at: 2315 2361
Size: 46
Fragments arranged by size:
1073 314 221 220 195 154 93 46
45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 612 2361
Size: 612 1749
BsrDI GCAATG_nn'
Cuts at: 0 1173 1720 1883 2361
Size: 1173 547 163 478
Fragments arranged by size:
1173 547 478 163
BsrFI r'CCGG_y
Cuts at: 0 866 2321 2361
Size: 866 1455 40
Fragments arranged by size:
1455 866 40
BsrGI T'GTAC_A
Cuts at: 0 338 2361
Size: 338 2023
BssSI C'ACGA_G
Cuts at: 0 1588 2361
Size: 1588 773
BstAPI GCAn_nnn'nTGC
Cuts at: 0 201 717 2361
Size: 201 516 1644
Fragments arranged by size:
1644 516 201
BstEII G'GTnAC_C
Cuts at: 0 981 2361
Size: 981 1380
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 741 2175 2361
Size: 312 429 1434 186
Fragments arranged by size:
1434 429 312 186
BstYI r'GATC_y
Cuts at: 0 138 542 875 1184 2342 2361
Size: 138 404 333 309 1158 19
Fragments arranged by size:
1158 404 333 309 138 19
BstZ17I GTA'TAC
Cuts at: 0 2034 2361
Size: 2034 327
Bsu36I CC'TnA_GG
Cuts at: 0 538 1016 2361
Size: 538 478 1345
Fragments arranged by size:
1345 538 478
BtgI C'CryG_G
Cuts at: 0 305 2361
Size: 305 2056
BtsI GCAGTG_nn'
Cuts at: 0 661 2361
Size: 661 1700
DraIII CAC_nnn'GTG
Cuts at: 0 225 2361
Size: 225 2136
EaeI y'GGCC_r
Cuts at: 0 66 686 782 845 938 1436 1510 2017
Size: 66 620 96 63 93 498 74 507
Cuts at: 2017 2323 2361
Size: 306 38
Fragments arranged by size:
620 507 498 306 96 93 74 66
63 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 316 2361
Size: 316 2045
EcoICRI GAG'CTC
Cuts at: 0 2313 2361
Size: 2313 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 265 268 368 409 595 652 1045
Size: 202 63 3 100 41 186 57 393
Cuts at: 1045 1230 1654 2296 2361
Size: 185 424 642 65
Fragments arranged by size:
642 424 393 202 186 185 100 65
63 57 41 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 193 1576 2361
Size: 193 1383 785
Fragments arranged by size:
1383 785 193
EcoO109I rG'GnC_Cy
Cuts at: 0 186 797 1020 1569 1838 2184 2361
Size: 186 611 223 549 269 346 177
Fragments arranged by size:
611 549 346 269 223 186 177
FseI GG_CCGG'CC
Cuts at: 0 2325 2361
Size: 2325 36
HaeII r_GCGC'y
Cuts at: 0 1531 2168 2361
Size: 1531 637 193
Fragments arranged by size:
1531 637 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 394 426 534 566 955 987
Size: 3 32 359 32 108 32 389 32
Cuts at: 987 2361
Size: 1374
Fragments arranged by size:
1374 389 359 108 32 32 32 32
3
HincII GTy'rAC
Cuts at: 0 948 2361
Size: 948 1413
MscI TGG'CCA
Cuts at: 0 68 1512 2361
Size: 68 1444 849
Fragments arranged by size:
1444 849 68
MslI CAynn'nnrTG
Cuts at: 0 85 1518 1766 2173 2361
Size: 85 1433 248 407 188
Fragments arranged by size:
1433 407 248 188 85
MspA1I CmG'CkG
Cuts at: 0 299 320 388 564 573 714 780 906
Size: 299 21 68 176 9 141 66 126
Cuts at: 906 1518 2197 2361
Size: 612 679 164
Fragments arranged by size:
679 612 299 176 164 141 126 68
66 21 9
NaeI GCC'GGC
Cuts at: 0 2323 2361
Size: 2323 38
NcoI C'CATG_G
Cuts at: 0 305 2361
Size: 305 2056
NgoMIV G'CCGG_C
Cuts at: 0 2321 2361
Size: 2321 40
NspI r_CATG'y
Cuts at: 0 2226 2361
Size: 2226 135
PciI A'CATG_T
Cuts at: 0 2222 2361
Size: 2222 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 1889 2361
Size: 180 1709 472
Fragments arranged by size:
1709 472 180
PfoI T'CCnGG_A
Cuts at: 0 179 740 2361
Size: 179 561 1621
Fragments arranged by size:
1621 561 179
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2227 2259 2361
Size: 2227 32 102
Fragments arranged by size:
2227 102 32
PpuMI rG'GwC_Cy
Cuts at: 0 1020 1838 2361
Size: 1020 818 523
Fragments arranged by size:
1020 818 523
PspOMI G'GGCC_C
Cuts at: 0 796 2361
Size: 796 1565
PstI C_TGCA'G
Cuts at: 0 206 497 668 713 2148 2361
Size: 206 291 171 45 1435 213
Fragments arranged by size:
1435 291 213 206 171 45
PvuII CAG'CTG
Cuts at: 0 388 573 714 780 906 1518 2197 2361
Size: 388 185 141 66 126 612 679 164
Fragments arranged by size:
679 612 388 185 164 141 126 66
SacI G_AGCT'C
Cuts at: 0 2315 2361
Size: 2315 46
ScaI AGT'ACT
Cuts at: 0 511 2361
Size: 511 1850
SexAI A'CCwGG_T
Cuts at: 0 221 2361
Size: 221 2140
SfcI C'TryA_G
Cuts at: 0 202 493 664 709 823 1641 2144 2361
Size: 202 291 171 45 114 818 503 217
Fragments arranged by size:
818 503 291 217 202 171 114 45
SmlI C'TyrA_G
Cuts at: 0 1147 1753 2361
Size: 1147 606 608
Fragments arranged by size:
1147 608 606
StuI AGG'CCT
Cuts at: 0 637 2361
Size: 637 1724
StyI C'CwwG_G
Cuts at: 0 30 305 1577 2361
Size: 30 275 1272 784
Fragments arranged by size:
1272 784 275 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 1949 2361
Size: 187 1762 412
Fragments arranged by size:
1762 412 187
TatI w'GTAC_w
Cuts at: 0 338 509 2361
Size: 338 171 1852
Fragments arranged by size:
1852 338 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2040 2072 2323 2355 2361
Size: 2040 32 251 32 6
Fragments arranged by size:
2040 251 32 32 6
Tth111I GACn'n_nGTC
Cuts at: 0 1383 2245 2361
Size: 1383 862 116
Fragments arranged by size:
1383 862 116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 177 553 1345 2361
Size: 177 376 792 1016
Fragments arranged by size:
1016 792 376 177
XmnI GAAnn'nnTTC
Cuts at: 0 41 1609 2361
Size: 41 1568 752
Fragments arranged by size:
1568 752 41
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BciVI BglII
BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI DraIII EaeI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI ScaI
SexAI SfcI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BcgI BclI BfrBI BglI BmtI BplI Bpu10I BsaI
BsaBI BsaHI BsaWI BsiWI BsmI BspEI BspHI BsrBI
BssHII BstBI BtgZI ClaI CspCI DraI DrdI EagI
EarI EcoRI EcoRV FalI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI NarI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI
ZraI