Restriction map for
pF1KB3243
(Linear) (Six-base) MAPSORT of: KIBB3243.seq Check: 8635 from: 1 to: 2805
KIBB3243.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 589 2805
Size: 589 2216
AccI GT'mk_AC
Cuts at: 0 2552 2805
Size: 2552 253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 200 353 1046 1108 2187 2567 2805
Size: 190 10 153 693 62 1079 380 238
Fragments arranged by size:
1079 693 380 238 190 153 62 10
AleI CACnn'nnGTG
Cuts at: 0 1891 2805
Size: 1891 914
AlwNI CAG_nnn'CTG
Cuts at: 0 235 1878 2293 2805
Size: 235 1643 415 512
Fragments arranged by size:
1643 512 415 235
ApaI G_GGCC'C
Cuts at: 0 537 830 1246 2805
Size: 537 293 416 1559
Fragments arranged by size:
1559 537 416 293
ApaLI G'TGCA_C
Cuts at: 0 1826 2352 2805
Size: 1826 526 453
Fragments arranged by size:
1826 526 453
ApoI r'AATT_y
Cuts at: 0 318 889 2246 2805
Size: 318 571 1357 559
Fragments arranged by size:
1357 571 559 318
AvaI C'yCGr_G
Cuts at: 0 358 1004 2805
Size: 358 646 1801
Fragments arranged by size:
1801 646 358
BanI G'GyrC_C
Cuts at: 0 3 480 1192 1437 2380 2805
Size: 3 477 712 245 943 425
Fragments arranged by size:
943 712 477 425 245 3
BanII G_rGCy'C
Cuts at: 0 537 830 1246 1796 2109 2805
Size: 537 293 416 550 313 696
Fragments arranged by size:
696 550 537 416 313 293
BbeI G_GCGC'C
Cuts at: 0 7 2805
Size: 7 2798
BbsI GAAGACnn'nnnn_
Cuts at: 0 275 1745 2805
Size: 275 1470 1060
Fragments arranged by size:
1470 1060 275
BciVI GTATCCnnnnn_n'
Cuts at: 0 1596 2277 2805
Size: 1596 681 528
Fragments arranged by size:
1596 681 528
BclI T'GATC_A
Cuts at: 0 341 2805
Size: 341 2464
BfrBI ATG'CAT
Cuts at: 0 543 2272 2805
Size: 543 1729 533
Fragments arranged by size:
1729 543 533
BglII A'GATC_T
Cuts at: 0 32 1809 2495 2805
Size: 32 1777 686 310
Fragments arranged by size:
1777 686 310 32
Bme1580I G_kGCm'C
Cuts at: 0 537 830 1197 1246 1830 1888 2356 2805
Size: 537 293 367 49 584 58 468 449
Fragments arranged by size:
584 537 468 449 367 293 58 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 1671 1765 2370 2455 2805
Size: 1671 94 605 85 350
Fragments arranged by size:
1671 605 350 94 85
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 2805
Size: 433 2372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1874 1900 2203 2805
Size: 904 970 26 303 602
Fragments arranged by size:
970 904 602 303 26
Bpu10I CC'TnA_GC
Cuts at: 0 2385 2396 2805
Size: 2385 11 409
Fragments arranged by size:
2385 409 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1951 2095 2437 2805
Size: 919 1032 144 342 368
Fragments arranged by size:
1032 919 368 342 144
BsaAI yAC'GTr
Cuts at: 0 1113 2079 2805
Size: 1113 966 726
Fragments arranged by size:
1113 966 726
BsaBI GATnn'nnATC
Cuts at: 0 146 190 2805
Size: 146 44 2615
Fragments arranged by size:
2615 146 44
BsaHI Gr'CG_yC
Cuts at: 0 4 2805
Size: 4 2801
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1781 1811 2805
Size: 1781 30 994
Fragments arranged by size:
1781 994 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 2805
Size: 625 2180
BseYI C'CCAG_C
Cuts at: 0 394 2425 2805
Size: 394 2031 380
Fragments arranged by size:
2031 394 380
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 2805
Size: 316 2489
BsiHKAI G_wGCw'C
Cuts at: 0 859 1830 2356 2725 2805
Size: 859 971 526 369 80
Fragments arranged by size:
971 859 526 369 80
BsmI GAATG_Cn'
Cuts at: 0 2272 2274 2805
Size: 2272 2 531
Fragments arranged by size:
2272 531 2
Bsp1286I G_dGCh'C
Cuts at: 0 537 830 859 1197 1246 1796 1830 1888
Size: 537 293 29 338 49 550 34 58
Cuts at: 1888 2109 2356 2725 2805
Size: 221 247 369 80
Fragments arranged by size:
550 537 369 338 293 247 221 80
58 49 34 29
BspHI T'CATG_A
Cuts at: 0 1395 2805
Size: 1395 1410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 589 2805
Size: 589 2216
BsrDI GCAATG_nn'
Cuts at: 0 848 1946 2483 2805
Size: 848 1098 537 322
Fragments arranged by size:
1098 848 537 322
BsrFI r'CCGG_y
Cuts at: 0 1094 2054 2805
Size: 1094 960 751
Fragments arranged by size:
1094 960 751
BsrGI T'GTAC_A
Cuts at: 0 279 2805
Size: 279 2526
BstAPI GCAn_nnn'nTGC
Cuts at: 0 549 2805
Size: 549 2256
BstEII G'GTnAC_C
Cuts at: 0 412 2805
Size: 412 2393
BstXI CCAn_nnnn'nTGG
Cuts at: 0 905 2589 2805
Size: 905 1684 216
Fragments arranged by size:
1684 905 216
BstYI r'GATC_y
Cuts at: 0 32 1809 2495 2805
Size: 32 1777 686 310
Fragments arranged by size:
1777 686 310 32
Bsu36I CC'TnA_GG
Cuts at: 0 446 1621 2805
Size: 446 1175 1184
Fragments arranged by size:
1184 1175 446
BtgI C'CryG_G
Cuts at: 0 2132 2805
Size: 2132 673
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1908 2805
Size: 1908 897
BtsI GCAGTG_nn'
Cuts at: 0 1346 2138 2805
Size: 1346 792 667
Fragments arranged by size:
1346 792 667
DraIII CAC_nnn'GTG
Cuts at: 0 2082 2805
Size: 2082 723
EaeI y'GGCC_r
Cuts at: 0 1373 1694 2261 2805
Size: 1373 321 567 544
Fragments arranged by size:
1373 567 544 321
EarI CTCTTCn'nnn_
Cuts at: 0 176 1022 1301 1532 2159 2805
Size: 176 846 279 231 627 646
Fragments arranged by size:
846 646 627 279 231 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 200 353 904 1046 1108 1874 1900
Size: 190 10 153 551 142 62 766 26
Cuts at: 1900 2187 2203 2567 2805
Size: 287 16 364 238
Fragments arranged by size:
766 551 364 287 238 190 153 142
62 26 16 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2395 2805
Size: 2395 410
EcoO109I rG'GnC_Cy
Cuts at: 0 533 534 826 1242 1243 2805
Size: 533 1 292 416 1 1562
Fragments arranged by size:
1562 533 416 292 1 1
EcoRI G'AATT_C
Cuts at: 0 318 2246 2805
Size: 318 1928 559
Fragments arranged by size:
1928 559 318
EcoRV GAT'ATC
Cuts at: 0 1686 2805
Size: 1686 1119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 471 1440 2400 2583 2805
Size: 471 969 960 183 222
Fragments arranged by size:
969 960 471 222 183
HaeII r_GCGC'y
Cuts at: 0 7 1271 2805
Size: 7 1264 1534
Fragments arranged by size:
1534 1264 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 585 617 1781 1813 2275 2307 2805
Size: 585 32 1164 32 462 32 498
Fragments arranged by size:
1164 585 498 462 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1969 2805
Size: 1969 836
KasI G'GCGC_C
Cuts at: 0 3 2805
Size: 3 2802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1580 1672 2805
Size: 1159 421 92 1133
Fragments arranged by size:
1159 1133 421 92
MscI TGG'CCA
Cuts at: 0 1696 2805
Size: 1696 1109
MslI CAynn'nnrTG
Cuts at: 0 605 634 1300 1891 2026 2587 2805
Size: 605 29 666 591 135 561 218
Fragments arranged by size:
666 605 591 561 218 135 29
NarI GG'CG_CC
Cuts at: 0 4 2805
Size: 4 2801
NruI TCG'CGA
Cuts at: 0 2666 2805
Size: 2666 139
NsiI A_TGCA'T
Cuts at: 0 545 2274 2805
Size: 545 1729 531
Fragments arranged by size:
1729 545 531
NspI r_CATG'y
Cuts at: 0 502 2805
Size: 502 2303
PasI CC'CwG_GG
Cuts at: 0 1143 2805
Size: 1143 1662
PflMI CCAn_nnn'nTGG
Cuts at: 0 1586 2082 2805
Size: 1586 496 723
Fragments arranged by size:
1586 723 496
PfoI T'CCnGG_A
Cuts at: 0 881 2805
Size: 881 1924
PmlI CAC'GTG
Cuts at: 0 2079 2805
Size: 2079 726
PspOMI G'GGCC_C
Cuts at: 0 533 826 1242 2805
Size: 533 293 416 1563
Fragments arranged by size:
1563 533 416 293
PstI C_TGCA'G
Cuts at: 0 1275 1353 2805
Size: 1275 78 1452
Fragments arranged by size:
1452 1275 78
SexAI A'CCwGG_T
Cuts at: 0 93 2805
Size: 93 2712
SfcI C'TryA_G
Cuts at: 0 1271 1349 2642 2805
Size: 1271 78 1293 163
Fragments arranged by size:
1293 1271 163 78
SfoI GGC'GCC
Cuts at: 0 5 2805
Size: 5 2800
SmlI C'TyrA_G
Cuts at: 0 934 1004 1966 2110 2452 2805
Size: 934 70 962 144 342 353
Fragments arranged by size:
962 934 353 342 144 70
SphI G_CATG'C
Cuts at: 0 502 2805
Size: 502 2303
SspI AAT'ATT
Cuts at: 0 119 2805
Size: 119 2686
StyI C'CwwG_G
Cuts at: 0 1188 2159 2805
Size: 1188 971 646
Fragments arranged by size:
1188 971 646
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 748 2439 2805
Size: 748 1691 366
Fragments arranged by size:
1691 748 366
TatI w'GTAC_w
Cuts at: 0 248 279 2805
Size: 248 31 2526
Fragments arranged by size:
2526 248 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 795 827 2805
Size: 795 32 1978
Fragments arranged by size:
1978 795 32
XbaI T'CTAG_A
Cuts at: 0 1734 2805
Size: 1734 1071
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 879 996 1704 2805
Size: 879 117 708 1101
Fragments arranged by size:
1101 879 708 117
XhoI C'TCGA_G
Cuts at: 0 1004 2805
Size: 1004 1801
XmnI GAAnn'nnTTC
Cuts at: 0 2272 2522 2805
Size: 2272 250 283
Fragments arranged by size:
2272 283 250
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HindIII KasI MmeI MscI MslI NarI NruI
NsiI NspI PasI PflMI PfoI PmlI PspOMI PstI
SexAI SfcI SfoI SmlI SphI SspI StyI TaqII
TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BglI BlpI BmgBI BmtI BsaI BsaWI
BsiEI BsiWI BsmBI BspEI BsrBI BssHII BssSI BstBI
BstZ17I ClaI CspCI DraI DrdI EagI EciI EcoICRI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI PacI
PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI Tth111I XmaI ZraI