Restriction map for
pF1KB3238
(Linear) (Six-base) MAPSORT of: KIBB3238.seq Check: 5720 from: 1 to: 2196
KIBB3238.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1710 1766 2196
Size: 929 781 56 430
Fragments arranged by size:
929 781 430 56
AfeI AGC'GCT
Cuts at: 0 1960 2196
Size: 1960 236
AflIII A'CryG_T
Cuts at: 0 1298 2196
Size: 1298 898
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1064 1096 2196
Size: 1064 32 1100
Fragments arranged by size:
1100 1064 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1479 2149 2196
Size: 1479 670 47
Fragments arranged by size:
1479 670 47
ApaLI G'TGCA_C
Cuts at: 0 235 2196
Size: 235 1961
ApoI r'AATT_y
Cuts at: 0 668 1655 2196
Size: 668 987 541
Fragments arranged by size:
987 668 541
AvaI C'yCGr_G
Cuts at: 0 1502 2196
Size: 1502 694
BanII G_rGCy'C
Cuts at: 0 1722 2196
Size: 1722 474
BbsI GAAGACnn'nnnn_
Cuts at: 0 699 1289 1411 1427 2196
Size: 699 590 122 16 769
Fragments arranged by size:
769 699 590 122 16
BbvCI CC'TCA_GC
Cuts at: 0 412 1559 2093 2196
Size: 412 1147 534 103
Fragments arranged by size:
1147 534 412 103
BciVI GTATCCnnnnn_n'
Cuts at: 0 1866 2196
Size: 1866 330
BclI T'GATC_A
Cuts at: 0 2054 2196
Size: 2054 142
Bme1580I G_kGCm'C
Cuts at: 0 239 2196
Size: 239 1957
BmgBI CAC'GTC
Cuts at: 0 45 2196
Size: 45 2151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2088 2196
Size: 2088 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1719 2196
Size: 292 1427 477
Fragments arranged by size:
1427 477 292
Bpu10I CC'TnA_GC
Cuts at: 0 412 552 1559 1649 2093 2196
Size: 412 140 1007 90 444 103
Fragments arranged by size:
1007 444 412 140 103 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1193 2196
Size: 634 559 1003
Fragments arranged by size:
1003 634 559
BsaBI GATnn'nnATC
Cuts at: 0 218 2196
Size: 218 1978
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 997 1027 2196
Size: 997 30 1169
Fragments arranged by size:
1169 997 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 548 2196
Size: 548 1648
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 1468 2196
Size: 1285 183 728
Fragments arranged by size:
1285 728 183
BsiEI CG_ry'CG
Cuts at: 0 10 2196
Size: 10 2186
BsiHKAI G_wGCw'C
Cuts at: 0 239 2196
Size: 239 1957
BsmI GAATG_Cn'
Cuts at: 0 726 1359 2196
Size: 726 633 837
Fragments arranged by size:
837 726 633
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1737 2196
Size: 1737 459
Bsp1286I G_dGCh'C
Cuts at: 0 239 1722 2196
Size: 239 1483 474
Fragments arranged by size:
1483 474 239
BsrGI T'GTAC_A
Cuts at: 0 123 2196
Size: 123 2073
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1910 2196
Size: 1910 286
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1386 1678 2196
Size: 1386 292 518
Fragments arranged by size:
1386 518 292
BtgI C'CryG_G
Cuts at: 0 561 2196
Size: 561 1635
BtsI GCAGTG_nn'
Cuts at: 0 1758 2196
Size: 1758 438
ClaI AT'CG_AT
Cuts at: 0 796 2196
Size: 796 1400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2115 2150 2196
Size: 2115 35 46
Fragments arranged by size:
2115 46 35
DraI TTT'AAA
Cuts at: 0 917 2196
Size: 917 1279
EaeI y'GGCC_r
Cuts at: 0 558 1169 2196
Size: 558 611 1027
Fragments arranged by size:
1027 611 558
EarI CTCTTCn'nnn_
Cuts at: 0 379 1894 1946 2196
Size: 379 1515 52 250
Fragments arranged by size:
1515 379 250 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 929 1710 1719 1766 2196
Size: 292 637 781 9 47 430
Fragments arranged by size:
781 637 430 292 47 9
EcoRV GAT'ATC
Cuts at: 0 638 2196
Size: 638 1558
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 628 1839 2196
Size: 628 1211 357
Fragments arranged by size:
1211 628 357
HaeII r_GCGC'y
Cuts at: 0 1962 2196
Size: 1962 234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 641 1456 1746 2196
Size: 318 323 815 290 450
Fragments arranged by size:
815 450 323 318 290
MscI TGG'CCA
Cuts at: 0 560 1171 2196
Size: 560 611 1025
Fragments arranged by size:
1025 611 560
MslI CAynn'nnrTG
Cuts at: 0 232 346 1244 1676 2196
Size: 232 114 898 432 520
Fragments arranged by size:
898 520 432 232 114
MspA1I CmG'CkG
Cuts at: 0 25 438 1117 1269 1558 2092 2149 2196
Size: 25 413 679 152 289 534 57 47
Fragments arranged by size:
679 534 413 289 152 57 47 25
NcoI C'CATG_G
Cuts at: 0 561 2196
Size: 561 1635
NspI r_CATG'y
Cuts at: 0 1302 2196
Size: 1302 894
PciI A'CATG_T
Cuts at: 0 1298 2196
Size: 1298 898
PflMI CCAn_nnn'nTGG
Cuts at: 0 737 2196
Size: 737 1459
PfoI T'CCnGG_A
Cuts at: 0 320 1925 2036 2196
Size: 320 1605 111 160
Fragments arranged by size:
1605 320 160 111
PsiI TTA'TAA
Cuts at: 0 448 2196
Size: 448 1748
PstI C_TGCA'G
Cuts at: 0 1122 2196
Size: 1122 1074
PvuII CAG'CTG
Cuts at: 0 25 438 1117 1269 2092 2149 2196
Size: 25 413 679 152 823 57 47
Fragments arranged by size:
823 679 413 152 57 47 25
SapI GCTCTTCn'nnn_
Cuts at: 0 1894 1946 2196
Size: 1894 52 250
Fragments arranged by size:
1894 250 52
SexAI A'CCwGG_T
Cuts at: 0 297 2196
Size: 297 1899
SfcI C'TryA_G
Cuts at: 0 802 1118 1685 2196
Size: 802 316 567 511
Fragments arranged by size:
802 567 511 316
SmaI CCC'GGG
Cuts at: 0 1504 2196
Size: 1504 692
SmlI C'TyrA_G
Cuts at: 0 613 1208 2196
Size: 613 595 988
Fragments arranged by size:
988 613 595
SspI AAT'ATT
Cuts at: 0 401 2196
Size: 401 1795
StyI C'CwwG_G
Cuts at: 0 561 2196
Size: 561 1635
TatI w'GTAC_w
Cuts at: 0 123 2196
Size: 123 2073
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 180 212 997 1029 2196
Size: 180 32 785 32 1167
Fragments arranged by size:
1167 785 180 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2109 2196
Size: 2109 87
XmaI C'CCGG_G
Cuts at: 0 1502 2196
Size: 1502 694
XmnI GAAnn'nnTTC
Cuts at: 0 1015 2196
Size: 1015 1181
Enzymes that do cut:
AcuI AfeI AflIII AloI AlwNI ApaLI ApoI AvaI
BanII BbsI BbvCI BciVI BclI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BsrGI BstAPI BstXI BtgI
BtsI ClaI CspCI DraI EaeI EarI Eco57MI EcoRV
FalI HaeII MmeI MscI MslI MspA1I NcoI NspI
PciI PflMI PfoI PsiI PstI PvuII SapI SexAI
SfcI SmaI SmlI SspI StyI TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BcgI BfrBI BglI BglII BlpI
BmrI BmtI BsaI BsaAI BsaHI BsaWI BseYI BsiWI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI StuI SwaI TaqII Tth111I XbaI
XhoI ZraI