Restriction map for
pF1KB3233
(Linear) (Six-base) MAPSORT of: KIBB3233.seq Check: 6048 from: 1 to: 2340
KIBB3233.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1132 1596 2197 2267 2340
Size: 214 918 464 601 70 73
Fragments arranged by size:
918 601 464 214 73 70
AgeI A'CCGG_T
Cuts at: 0 130 2340
Size: 130 2210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1312 2340
Size: 1312 1028
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 744 776 2340
Size: 744 32 1564
Fragments arranged by size:
1564 744 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1210 1681 2170 2340
Size: 1210 471 489 170
Fragments arranged by size:
1210 489 471 170
ApoI r'AATT_y
Cuts at: 0 929 1096 2019 2340
Size: 929 167 923 321
Fragments arranged by size:
929 923 321 167
AvaI C'yCGr_G
Cuts at: 0 872 2340
Size: 872 1468
BanI G'GyrC_C
Cuts at: 0 133 511 1684 2038 2340
Size: 133 378 1173 354 302
Fragments arranged by size:
1173 378 354 302 133
BanII G_rGCy'C
Cuts at: 0 1230 1553 1796 2002 2151 2340
Size: 1230 323 243 206 149 189
Fragments arranged by size:
1230 323 243 206 189 149
BbeI G_GCGC'C
Cuts at: 0 2042 2340
Size: 2042 298
BbsI GAAGACnn'nnnn_
Cuts at: 0 135 836 2148 2340
Size: 135 701 1312 192
Fragments arranged by size:
1312 701 192 135
BbvCI CC'TCA_GC
Cuts at: 0 788 2340
Size: 788 1552
BciVI GTATCCnnnnn_n'
Cuts at: 0 631 2340
Size: 631 1709
BglI GCCn_nnn'nGGC
Cuts at: 0 1388 2340
Size: 1388 952
BlpI GC'TnA_GC
Cuts at: 0 2171 2340
Size: 2171 169
Bme1580I G_kGCm'C
Cuts at: 0 138 261 2340
Size: 138 123 2079
Fragments arranged by size:
2079 138 123
BmrI ACTGGGnnnn_n'
Cuts at: 0 377 687 723 1152 2340
Size: 377 310 36 429 1188
Fragments arranged by size:
1188 429 377 310 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1377 2096 2320 2340
Size: 400 977 719 224 20
Fragments arranged by size:
977 719 400 224 20
Bpu10I CC'TnA_GC
Cuts at: 0 788 2340
Size: 788 1552
BsaI GGTCTCn'nnnn_
Cuts at: 0 1802 2340
Size: 1802 538
BsaHI Gr'CG_yC
Cuts at: 0 910 2039 2340
Size: 910 1129 301
Fragments arranged by size:
1129 910 301
BsaWI w'CCGG_w
Cuts at: 0 130 2340
Size: 130 2210
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 230 260 2340
Size: 230 30 2080
Fragments arranged by size:
2080 230 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 457 1804 1951 2340
Size: 457 1347 147 389
Fragments arranged by size:
1347 457 389 147
BseYI C'CCAG_C
Cuts at: 0 234 993 1762 2340
Size: 234 759 769 578
Fragments arranged by size:
769 759 578 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1632 2340
Size: 916 716 708
Fragments arranged by size:
916 716 708
BsiEI CG_ry'CG
Cuts at: 0 2336 2340
Size: 2336 4
BsiHKAI G_wGCw'C
Cuts at: 0 17 1301 1553 2306 2340
Size: 17 1284 252 753 34
Fragments arranged by size:
1284 753 252 34 17
BsmI GAATG_Cn'
Cuts at: 0 96 1251 2340
Size: 96 1155 1089
Fragments arranged by size:
1155 1089 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 772 2340
Size: 772 1568
Bsp1286I G_dGCh'C
Cuts at: 0 17 138 261 1230 1301 1553 1796 2002
Size: 17 121 123 969 71 252 243 206
Cuts at: 2002 2151 2306 2340
Size: 149 155 34
Fragments arranged by size:
969 252 243 206 155 149 123 121
71 34 17
BspHI T'CATG_A
Cuts at: 0 1133 2340
Size: 1133 1207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 415 1820 2340
Size: 415 1405 520
Fragments arranged by size:
1405 520 415
BsrDI GCAATG_nn'
Cuts at: 0 50 1899 2340
Size: 50 1849 441
Fragments arranged by size:
1849 441 50
BsrFI r'CCGG_y
Cuts at: 0 130 2340
Size: 130 2210
BsrGI T'GTAC_A
Cuts at: 0 1151 2340
Size: 1151 1189
BstEII G'GTnAC_C
Cuts at: 0 1018 1059 2340
Size: 1018 41 1281
Fragments arranged by size:
1281 1018 41
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 1083 2034 2340
Size: 33 1050 951 306
Fragments arranged by size:
1050 951 306 33
BstYI r'GATC_y
Cuts at: 0 2096 2340
Size: 2096 244
BtgI C'CryG_G
Cuts at: 0 756 1082 1745 2340
Size: 756 326 663 595
Fragments arranged by size:
756 663 595 326
BtsI GCAGTG_nn'
Cuts at: 0 581 965 2340
Size: 581 384 1375
Fragments arranged by size:
1375 581 384
DraIII CAC_nnn'GTG
Cuts at: 0 1206 2340
Size: 1206 1134
EaeI y'GGCC_r
Cuts at: 0 1328 1380 2333 2340
Size: 1328 52 953 7
Fragments arranged by size:
1328 953 52 7
EagI C'GGCC_G
Cuts at: 0 2333 2340
Size: 2333 7
EarI CTCTTCn'nnn_
Cuts at: 0 5 1420 2340
Size: 5 1415 920
Fragments arranged by size:
1415 920 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2109 2340
Size: 2109 231
EcoICRI GAG'CTC
Cuts at: 0 1551 2340
Size: 1551 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 400 1132 1377 1596 2096 2197 2267
Size: 214 186 732 245 219 500 101 70
Cuts at: 2267 2320 2340
Size: 53 20
Fragments arranged by size:
732 500 245 219 214 186 101 70
53 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1282 1330 2340
Size: 1282 48 1010
Fragments arranged by size:
1282 1010 48
EcoO109I rG'GnC_Cy
Cuts at: 0 752 980 1323 1473 1512 2340
Size: 752 228 343 150 39 828
Fragments arranged by size:
828 752 343 228 150 39
EcoRI G'AATT_C
Cuts at: 0 929 2340
Size: 929 1411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1446 1968 2340
Size: 1446 522 372
Fragments arranged by size:
1446 522 372
HaeII r_GCGC'y
Cuts at: 0 2042 2340
Size: 2042 298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 107 139 176 208 318 350 2340
Size: 107 32 37 32 110 32 1990
Fragments arranged by size:
1990 110 107 37 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1156 2340
Size: 1156 1184
KasI G'GCGC_C
Cuts at: 0 2038 2340
Size: 2038 302
MfeI C'AATT_G
Cuts at: 0 491 797 2340
Size: 491 306 1543
Fragments arranged by size:
1543 491 306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1613 2184 2340
Size: 1613 571 156
Fragments arranged by size:
1613 571 156
MscI TGG'CCA
Cuts at: 0 1330 1382 2340
Size: 1330 52 958
Fragments arranged by size:
1330 958 52
MslI CAynn'nnrTG
Cuts at: 0 517 781 2032 2340
Size: 517 264 1251 308
Fragments arranged by size:
1251 517 308 264
MspA1I CmG'CkG
Cuts at: 0 234 1681 1762 2340
Size: 234 1447 81 578
Fragments arranged by size:
1447 578 234 81
NarI GG'CG_CC
Cuts at: 0 2039 2340
Size: 2039 301
NcoI C'CATG_G
Cuts at: 0 1082 1745 2340
Size: 1082 663 595
Fragments arranged by size:
1082 663 595
NspI r_CATG'y
Cuts at: 0 895 1986 2340
Size: 895 1091 354
Fragments arranged by size:
1091 895 354
PasI CC'CwG_GG
Cuts at: 0 33 2340
Size: 33 2307
PflMI CCAn_nnn'nTGG
Cuts at: 0 2166 2340
Size: 2166 174
PpuMI rG'GwC_Cy
Cuts at: 0 752 980 2340
Size: 752 228 1360
Fragments arranged by size:
1360 752 228
PstI C_TGCA'G
Cuts at: 0 1239 1817 2340
Size: 1239 578 523
Fragments arranged by size:
1239 578 523
PvuII CAG'CTG
Cuts at: 0 234 1681 1762 2340
Size: 234 1447 81 578
Fragments arranged by size:
1447 578 234 81
RsrII CG'GwC_CG
Cuts at: 0 2321 2340
Size: 2321 19
SacI G_AGCT'C
Cuts at: 0 1553 2340
Size: 1553 787
SanDI GG'GwC_CC
Cuts at: 0 980 2340
Size: 980 1360
SapI GCTCTTCn'nnn_
Cuts at: 0 5 1420 2340
Size: 5 1415 920
Fragments arranged by size:
1415 920 5
SbfI CC_TGCA'GG
Cuts at: 0 1817 2340
Size: 1817 523
SexAI A'CCwGG_T
Cuts at: 0 311 2340
Size: 311 2029
SfcI C'TryA_G
Cuts at: 0 1235 1580 1813 2340
Size: 1235 345 233 527
Fragments arranged by size:
1235 527 345 233
SfoI GGC'GCC
Cuts at: 0 2040 2340
Size: 2040 300
SgrAI Cr'CCGG_yG
Cuts at: 0 130 2340
Size: 130 2210
SmaI CCC'GGG
Cuts at: 0 874 2340
Size: 874 1466
StuI AGG'CCT
Cuts at: 0 170 2340
Size: 170 2170
StyI C'CwwG_G
Cuts at: 0 81 1076 1082 1331 1383 1745 2042 2210
Size: 81 995 6 249 52 362 297 168
Cuts at: 2210 2340
Size: 130
Fragments arranged by size:
995 362 297 249 168 130 81 52
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 937 1708 2340
Size: 937 771 632
Fragments arranged by size:
937 771 632
TatI w'GTAC_w
Cuts at: 0 1151 1928 2340
Size: 1151 777 412
Fragments arranged by size:
1151 777 412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 2206 2238 2340
Size: 230 32 1944 32 102
Fragments arranged by size:
1944 230 102 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 710 1752 2340
Size: 710 1042 588
Fragments arranged by size:
1042 710 588
XmaI C'CCGG_G
Cuts at: 0 872 2340
Size: 872 1468
Enzymes that do cut:
AcuI AgeI AlfI AloI AlwNI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstEII BstXI BstYI BtgI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII KasI
MfeI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PpuMI PstI PvuII RsrII SacI SanDI
SapI SbfI SexAI SfcI SfoI SgrAI SmaI StuI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BclI BfrBI BglII BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsiWI BspEI BsrBI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EcoRV FseI FspI FspAI HincII HpaI
KpnI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI SacII SalI ScaI
SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI ZraI