Restriction map for
pF1KB3222
(Linear) (Six-base) MAPSORT of: KIBB3222.seq Check: 3223 from: 1 to: 2937
KIBB3222.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
Acc65I G'GTAC_C
Cuts at: 0 35 2937
Size: 35 2902
AclI AA'CG_TT
Cuts at: 0 1634 2937
Size: 1634 1303
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1170 2485 2506 2641 2937
Size: 1170 1315 21 135 296
Fragments arranged by size:
1315 1170 296 135 21
AflIII A'CryG_T
Cuts at: 0 297 583 2937
Size: 297 286 2354
Fragments arranged by size:
2354 297 286
AhdI GACnn_n'nnGTC
Cuts at: 0 729 2937
Size: 729 2208
AleI CACnn'nnGTG
Cuts at: 0 664 1321 2937
Size: 664 657 1616
Fragments arranged by size:
1616 664 657
AlwNI CAG_nnn'CTG
Cuts at: 0 2397 2739 2835 2937
Size: 2397 342 96 102
Fragments arranged by size:
2397 342 102 96
ApaLI G'TGCA_C
Cuts at: 0 655 1254 2937
Size: 655 599 1683
Fragments arranged by size:
1683 655 599
ApoI r'AATT_y
Cuts at: 0 70 1699 2505 2937
Size: 70 1629 806 432
Fragments arranged by size:
1629 806 432 70
AvaI C'yCGr_G
Cuts at: 0 123 1024 1358 2937
Size: 123 901 334 1579
Fragments arranged by size:
1579 901 334 123
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 349 382 2937
Size: 349 33 2555
Fragments arranged by size:
2555 349 33
BanI G'GyrC_C
Cuts at: 0 35 925 2937
Size: 35 890 2012
Fragments arranged by size:
2012 890 35
BanII G_rGCy'C
Cuts at: 0 595 1406 2937
Size: 595 811 1531
Fragments arranged by size:
1531 811 595
BbsI GAAGACnn'nnnn_
Cuts at: 0 335 461 1323 2937
Size: 335 126 862 1614
Fragments arranged by size:
1614 862 335 126
BbvCI CC'TCA_GC
Cuts at: 0 2380 2937
Size: 2380 557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 284 318 2580 2614 2937
Size: 284 34 2262 34 323
Fragments arranged by size:
2262 323 284 34 34
BclI T'GATC_A
Cuts at: 0 824 2937
Size: 824 2113
BglII A'GATC_T
Cuts at: 0 1796 2937
Size: 1796 1141
BlpI GC'TnA_GC
Cuts at: 0 2749 2937
Size: 2749 188
Bme1580I G_kGCm'C
Cuts at: 0 659 1258 2937
Size: 659 599 1679
Fragments arranged by size:
1679 659 599
BmgBI CAC'GTC
Cuts at: 0 584 1671 2690 2937
Size: 584 1087 1019 247
Fragments arranged by size:
1087 1019 584 247
BmrI ACTGGGnnnn_n'
Cuts at: 0 735 2937
Size: 735 2202
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1006 1782 2937
Size: 1006 776 1155
Fragments arranged by size:
1155 1006 776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 70 382 867 1084 1837 2278 2712
Size: 61 9 312 485 217 753 441 434
Cuts at: 2712 2937
Size: 225
Fragments arranged by size:
753 485 441 434 312 225 217 61
9
Bpu10I CC'TnA_GC
Cuts at: 0 994 2380 2906 2937
Size: 994 1386 526 31
Fragments arranged by size:
1386 994 526 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 236 1044 2937
Size: 236 808 1893
Fragments arranged by size:
1893 808 236
BsaAI yAC'GTr
Cuts at: 0 298 429 2937
Size: 298 131 2508
Fragments arranged by size:
2508 298 131
BsaBI GATnn'nnATC
Cuts at: 0 1960 2937
Size: 1960 977
BsaHI Gr'CG_yC
Cuts at: 0 730 2937
Size: 730 2207
BsaWI w'CCGG_w
Cuts at: 0 530 2937
Size: 530 2407
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 607 637 2020 2050 2937
Size: 184 30 393 30 1383 30 887
Fragments arranged by size:
1383 887 393 184 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 358 822 1468 1930 2089 2092 2121
Size: 205 153 464 646 462 159 3 29
Cuts at: 2121 2937
Size: 816
Fragments arranged by size:
816 646 464 462 205 159 153 29
3
BseYI C'CCAG_C
Cuts at: 0 1660 1873 2702 2937
Size: 1660 213 829 235
Fragments arranged by size:
1660 829 235 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 939 2131 2937
Size: 939 1192 806
Fragments arranged by size:
1192 939 806
BsiHKAI G_wGCw'C
Cuts at: 0 530 659 941 1258 1379 2215 2937
Size: 530 129 282 317 121 836 722
Fragments arranged by size:
836 722 530 317 282 129 121
BsmI GAATG_Cn'
Cuts at: 0 468 642 2937
Size: 468 174 2295
Fragments arranged by size:
2295 468 174
BsmBI CGTCTCn'nnnn_
Cuts at: 0 520 1661 2937
Size: 520 1141 1276
Fragments arranged by size:
1276 1141 520
Bsp1286I G_dGCh'C
Cuts at: 0 530 595 659 941 1258 1379 1406 2215
Size: 530 65 64 282 317 121 27 809
Cuts at: 2215 2937
Size: 722
Fragments arranged by size:
809 722 530 317 282 121 65 64
27
BspHI T'CATG_A
Cuts at: 0 827 2786 2937
Size: 827 1959 151
Fragments arranged by size:
1959 827 151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1610 2591 2937
Size: 1610 981 346
Fragments arranged by size:
1610 981 346
BsrBI CCG'CTC
Cuts at: 0 951 978 2937
Size: 951 27 1959
Fragments arranged by size:
1959 951 27
BssSI C'ACGA_G
Cuts at: 0 1047 2937
Size: 1047 1890
BstAPI GCAn_nnn'nTGC
Cuts at: 0 20 1080 2937
Size: 20 1060 1857
Fragments arranged by size:
1857 1060 20
BstBI TT'CG_AA
Cuts at: 0 304 1703 2937
Size: 304 1399 1234
Fragments arranged by size:
1399 1234 304
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 612 1881 1980 2302 2937
Size: 39 573 1269 99 322 635
Fragments arranged by size:
1269 635 573 322 99 39
BstYI r'GATC_y
Cuts at: 0 156 218 1106 1796 1865 2706 2937
Size: 156 62 888 690 69 841 231
Fragments arranged by size:
888 841 690 231 156 69 62
Bsu36I CC'TnA_GG
Cuts at: 0 1465 1507 2937
Size: 1465 42 1430
Fragments arranged by size:
1465 1430 42
BtgI C'CryG_G
Cuts at: 0 206 662 1319 1937 2937
Size: 206 456 657 618 1000
Fragments arranged by size:
1000 657 618 456 206
BtsI GCAGTG_nn'
Cuts at: 0 2119 2694 2937
Size: 2119 575 243
Fragments arranged by size:
2119 575 243
DraIII CAC_nnn'GTG
Cuts at: 0 663 1223 2937
Size: 663 560 1714
Fragments arranged by size:
1714 663 560
DrdI GACnn_nn'nnGTC
Cuts at: 0 1675 2782 2937
Size: 1675 1107 155
Fragments arranged by size:
1675 1107 155
EaeI y'GGCC_r
Cuts at: 0 29 209 563 704 2937
Size: 29 180 354 141 2233
Fragments arranged by size:
2233 354 180 141 29
EarI CTCTTCn'nnn_
Cuts at: 0 335 898 1219 1604 2066 2937
Size: 335 563 321 385 462 871
Fragments arranged by size:
871 563 462 385 335 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1504 2937
Size: 1504 1433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 70 382 867 1084 1170 1837 2278
Size: 61 9 312 485 217 86 667 441
Cuts at: 2278 2485 2506 2641 2712 2937
Size: 207 21 135 71 225
Fragments arranged by size:
667 485 441 312 225 217 207 135
86 71 61 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1450 2937
Size: 1450 1487
EcoO109I rG'GnC_Cy
Cuts at: 0 45 819 1209 1763 2193 2262 2937
Size: 45 774 390 554 430 69 675
Fragments arranged by size:
774 675 554 430 390 69 45
EcoRI G'AATT_C
Cuts at: 0 2505 2937
Size: 2505 432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 99 2937
Size: 99 2838
HaeII r_GCGC'y
Cuts at: 0 2337 2937
Size: 2337 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 500 532 715 747 2020 2052 2937
Size: 500 32 183 32 1273 32 885
Fragments arranged by size:
1273 885 500 183 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1654 2653 2937
Size: 1654 999 284
Fragments arranged by size:
1654 999 284
KpnI G_GTAC'C
Cuts at: 0 39 2937
Size: 39 2898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1484 2645 2937
Size: 701 783 1161 292
Fragments arranged by size:
1161 783 701 292
MscI TGG'CCA
Cuts at: 0 31 565 706 2937
Size: 31 534 141 2231
Fragments arranged by size:
2231 534 141 31
MslI CAynn'nnrTG
Cuts at: 0 169 211 610 664 1321 1564 1978 2937
Size: 169 42 399 54 657 243 414 959
Fragments arranged by size:
959 657 414 399 243 169 54 42
MspA1I CmG'CkG
Cuts at: 0 20 1321 1881 2937
Size: 20 1301 560 1056
Fragments arranged by size:
1301 1056 560 20
NcoI C'CATG_G
Cuts at: 0 206 1937 2937
Size: 206 1731 1000
Fragments arranged by size:
1731 1000 206
NspI r_CATG'y
Cuts at: 0 1102 2061 2937
Size: 1102 959 876
Fragments arranged by size:
1102 959 876
PasI CC'CwG_GG
Cuts at: 0 2040 2266 2937
Size: 2040 226 671
Fragments arranged by size:
2040 671 226
PflMI CCAn_nnn'nTGG
Cuts at: 0 2397 2937
Size: 2397 540
PfoI T'CCnGG_A
Cuts at: 0 1574 2937
Size: 1574 1363
PmlI CAC'GTG
Cuts at: 0 298 2937
Size: 298 2639
PpuMI rG'GwC_Cy
Cuts at: 0 45 819 1209 1763 2262 2937
Size: 45 774 390 554 499 675
Fragments arranged by size:
774 675 554 499 390 45
PshAI GACnn'nnGTC
Cuts at: 0 2917 2937
Size: 2917 20
PstI C_TGCA'G
Cuts at: 0 1076 1355 1422 1484 1865 2831 2937
Size: 1076 279 67 62 381 966 106
Fragments arranged by size:
1076 966 381 279 106 67 62
PvuII CAG'CTG
Cuts at: 0 1881 2937
Size: 1881 1056
SacII CC_GC'GG
Cuts at: 0 1322 2937
Size: 1322 1615
SanDI GG'GwC_CC
Cuts at: 0 2262 2937
Size: 2262 675
ScaI AGT'ACT
Cuts at: 0 1141 1153 2937
Size: 1141 12 1784
Fragments arranged by size:
1784 1141 12
SfcI C'TryA_G
Cuts at: 0 1072 1351 1418 1480 1551 1861 2827 2937
Size: 1072 279 67 62 71 310 966 110
Fragments arranged by size:
1072 966 310 279 110 71 67 62
SmaI CCC'GGG
Cuts at: 0 1360 2937
Size: 1360 1577
StuI AGG'CCT
Cuts at: 0 1030 2937
Size: 1030 1907
StyI C'CwwG_G
Cuts at: 0 206 1937 2063 2372 2937
Size: 206 1731 126 309 565
Fragments arranged by size:
1731 565 309 206 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 463 870 2937
Size: 463 407 2067
Fragments arranged by size:
2067 463 407
TatI w'GTAC_w
Cuts at: 0 416 1139 1151 1595 2937
Size: 416 723 12 444 1342
Fragments arranged by size:
1342 723 444 416 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 184 216 2161 2193 2937
Size: 184 32 1945 32 744
Fragments arranged by size:
1945 744 184 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 512 2937
Size: 512 2425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1053 2937
Size: 1053 1884
XmaI C'CCGG_G
Cuts at: 0 1358 2937
Size: 1358 1579
XmnI GAAnn'nnTTC
Cuts at: 0 1519 2888 2937
Size: 1519 1369 49
Fragments arranged by size:
1519 1369 49
Enzymes that do cut:
Acc65I AclI AcuI AflIII AhdI AleI AlwNI ApaLI
ApoI AvaI BaeI BanI BanII BbsI BbvCI BcgI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HindIII KpnI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PmlI PpuMI PshAI PstI PvuII SacII SanDI ScaI
SfcI SmaI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AgeI AlfI AloI
ApaI AscI AseI AsiSI AvrII BamHI BbeI BciVI
BfrBI BglI BmtI BpuEI BsiEI BsiWI BspEI BsrDI
BsrFI BsrGI BssHII BstEII BstZ17I BtgZI ClaI CspCI
DraI EagI EcoICRI EcoRV FseI FspI FspAI HincII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI
ZraI