Restriction map for
pF1KB3200
(Linear) (Six-base) MAPSORT of: KIBB3200.seq Check: 8359 from: 1 to: 2037
KIBB3200.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 413 898 1727 1775 1903 1954 2037
Size: 248 165 485 829 48 128 51 83
Fragments arranged by size:
829 485 248 165 128 83 51 48
AflIII A'CryG_T
Cuts at: 0 1590 2037
Size: 1590 447
AhdI GACnn_n'nnGTC
Cuts at: 0 325 2037
Size: 325 1712
AleI CACnn'nnGTG
Cuts at: 0 1300 2037
Size: 1300 737
AlwNI CAG_nnn'CTG
Cuts at: 0 676 2037
Size: 676 1361
ApaI G_GGCC'C
Cuts at: 0 61 2037
Size: 61 1976
ApoI r'AATT_y
Cuts at: 0 767 1520 1792 2037
Size: 767 753 272 245
Fragments arranged by size:
767 753 272 245
AseI AT'TA_AT
Cuts at: 0 659 1373 2037
Size: 659 714 664
Fragments arranged by size:
714 664 659
AvaI C'yCGr_G
Cuts at: 0 53 2037
Size: 53 1984
BanI G'GyrC_C
Cuts at: 0 40 247 2037
Size: 40 207 1790
Fragments arranged by size:
1790 207 40
BanII G_rGCy'C
Cuts at: 0 61 668 1218 1862 2037
Size: 61 607 550 644 175
Fragments arranged by size:
644 607 550 175 61
BbeI G_GCGC'C
Cuts at: 0 44 2037
Size: 44 1993
BbsI GAAGACnn'nnnn_
Cuts at: 0 713 833 1637 1718 2037
Size: 713 120 804 81 319
Fragments arranged by size:
804 713 319 120 81
BciVI GTATCCnnnnn_n'
Cuts at: 0 801 2037
Size: 801 1236
BclI T'GATC_A
Cuts at: 0 569 2037
Size: 569 1468
BfrBI ATG'CAT
Cuts at: 0 139 2037
Size: 139 1898
Bme1580I G_kGCm'C
Cuts at: 0 61 551 2037
Size: 61 490 1486
Fragments arranged by size:
1486 490 61
BmgBI CAC'GTC
Cuts at: 0 1621 2037
Size: 1621 416
BmrI ACTGGGnnnn_n'
Cuts at: 0 2009 2037
Size: 2009 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1502 2037
Size: 253 1249 535
Fragments arranged by size:
1249 535 253
BsaBI GATnn'nnATC
Cuts at: 0 1636 2037
Size: 1636 401
BsaHI Gr'CG_yC
Cuts at: 0 41 2037
Size: 41 1996
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 149 179 491 521 1074 1104 2037
Size: 149 30 312 30 553 30 933
Fragments arranged by size:
933 553 312 149 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 948 2037
Size: 948 1089
BseYI C'CCAG_C
Cuts at: 0 579 2037
Size: 579 1458
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 2037
Size: 988 1049
BsiEI CG_ry'CG
Cuts at: 0 68 2037
Size: 68 1969
BsiHKAI G_wGCw'C
Cuts at: 0 1047 1862 2037
Size: 1047 815 175
Fragments arranged by size:
1047 815 175
BsmI GAATG_Cn'
Cuts at: 0 243 311 2037
Size: 243 68 1726
Fragments arranged by size:
1726 243 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 37 370 2037
Size: 37 333 1667
Fragments arranged by size:
1667 333 37
Bsp1286I G_dGCh'C
Cuts at: 0 61 551 668 1047 1218 1862 2037
Size: 61 490 117 379 171 644 175
Fragments arranged by size:
644 490 379 175 171 117 61
BspHI T'CATG_A
Cuts at: 0 102 2037
Size: 102 1935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1818 2037
Size: 1818 219
BsrDI GCAATG_nn'
Cuts at: 0 716 2037
Size: 716 1321
BsrFI r'CCGG_y
Cuts at: 0 309 374 1285 1715 2037
Size: 309 65 911 430 322
Fragments arranged by size:
911 430 322 309 65
BsrGI T'GTAC_A
Cuts at: 0 1194 2037
Size: 1194 843
BssSI C'ACGA_G
Cuts at: 0 34 1535 2037
Size: 34 1501 502
Fragments arranged by size:
1501 502 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 562 2037
Size: 562 1475
BstBI TT'CG_AA
Cuts at: 0 1943 2037
Size: 1943 94
BstXI CCAn_nnnn'nTGG
Cuts at: 0 634 1648 2037
Size: 634 1014 389
Fragments arranged by size:
1014 634 389
BstYI r'GATC_y
Cuts at: 0 1200 2037
Size: 1200 837
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1304 2037
Size: 1304 733
DraI TTT'AAA
Cuts at: 0 426 2037
Size: 426 1611
DraIII CAC_nnn'GTG
Cuts at: 0 858 2037
Size: 858 1179
EaeI y'GGCC_r
Cuts at: 0 65 311 372 624 1250 1283 2037
Size: 65 246 61 252 626 33 754
Fragments arranged by size:
754 626 252 246 65 61 33
EagI C'GGCC_G
Cuts at: 0 65 2037
Size: 65 1972
EarI CTCTTCn'nnn_
Cuts at: 0 1396 1832 1922 2037
Size: 1396 436 90 115
Fragments arranged by size:
1396 436 115 90
EcoICRI GAG'CTC
Cuts at: 0 1860 2037
Size: 1860 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 253 413 898 1502 1727 1775 1903
Size: 248 5 160 485 604 225 48 128
Cuts at: 1903 1954 2037
Size: 51 83
Fragments arranged by size:
604 485 248 225 160 128 83 51
48 5
EcoO109I rG'GnC_Cy
Cuts at: 0 57 58 2037
Size: 57 1 1979
Fragments arranged by size:
1979 57 1
EcoRI G'AATT_C
Cuts at: 0 1520 1792 2037
Size: 1520 272 245
Fragments arranged by size:
1520 272 245
EcoRV GAT'ATC
Cuts at: 0 632 2037
Size: 632 1405
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1168 2037
Size: 1168 869
HaeII r_GCGC'y
Cuts at: 0 44 2037
Size: 44 1993
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 311 343 778 810 1029 1061
Size: 124 32 155 32 435 32 219 32
Cuts at: 1061 1074 1106 2037
Size: 13 32 931
Fragments arranged by size:
931 435 219 155 124 32 32 32
32 32 13
HincII GTy'rAC
Cuts at: 0 1037 2037
Size: 1037 1000
KasI G'GCGC_C
Cuts at: 0 40 2037
Size: 40 1997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1417 2037
Size: 282 1135 620
Fragments arranged by size:
1135 620 282
MscI TGG'CCA
Cuts at: 0 626 1252 2037
Size: 626 626 785
Fragments arranged by size:
785 626 626
MslI CAynn'nnrTG
Cuts at: 0 1300 2037
Size: 1300 737
MspA1I CmG'CkG
Cuts at: 0 673 1426 2037
Size: 673 753 611
Fragments arranged by size:
753 673 611
NaeI GCC'GGC
Cuts at: 0 311 376 1287 2037
Size: 311 65 911 750
Fragments arranged by size:
911 750 311 65
NarI GG'CG_CC
Cuts at: 0 41 2037
Size: 41 1996
NgoMIV G'CCGG_C
Cuts at: 0 309 374 1285 2037
Size: 309 65 911 752
Fragments arranged by size:
911 752 309 65
NsiI A_TGCA'T
Cuts at: 0 141 2037
Size: 141 1896
NspI r_CATG'y
Cuts at: 0 1594 2037
Size: 1594 443
PciI A'CATG_T
Cuts at: 0 1590 2037
Size: 1590 447
PfoI T'CCnGG_A
Cuts at: 0 761 2037
Size: 761 1276
PspOMI G'GGCC_C
Cuts at: 0 57 2037
Size: 57 1980
PstI C_TGCA'G
Cuts at: 0 24 534 2037
Size: 24 510 1503
Fragments arranged by size:
1503 510 24
PvuII CAG'CTG
Cuts at: 0 673 2037
Size: 673 1364
SacI G_AGCT'C
Cuts at: 0 1862 2037
Size: 1862 175
SapI GCTCTTCn'nnn_
Cuts at: 0 1396 2037
Size: 1396 641
SfcI C'TryA_G
Cuts at: 0 20 530 2037
Size: 20 510 1507
Fragments arranged by size:
1507 510 20
SfoI GGC'GCC
Cuts at: 0 42 2037
Size: 42 1995
SmaI CCC'GGG
Cuts at: 0 55 2037
Size: 55 1982
SspI AAT'ATT
Cuts at: 0 1896 2037
Size: 1896 141
StuI AGG'CCT
Cuts at: 0 841 2037
Size: 841 1196
StyI C'CwwG_G
Cuts at: 0 1178 1497 2037
Size: 1178 319 540
Fragments arranged by size:
1178 540 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1147 2037
Size: 1147 890
TatI w'GTAC_w
Cuts at: 0 396 1194 1587 2037
Size: 396 798 393 450
Fragments arranged by size:
798 450 396 393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 629 661 2037
Size: 491 32 106 32 1376
Fragments arranged by size:
1376 491 106 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 712 2037
Size: 712 1325
XbaI T'CTAG_A
Cuts at: 0 693 2037
Size: 693 1344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 195 1648 2037
Size: 195 1453 389
Fragments arranged by size:
1453 389 195
XmaI C'CCGG_G
Cuts at: 0 53 2037
Size: 53 1984
XmnI GAAnn'nnTTC
Cuts at: 0 865 1148 1939 2037
Size: 865 283 791 98
Fragments arranged by size:
865 791 283 98
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApaI ApoI AseI
AvaI BanI BanII BbeI BbsI BciVI BclI BfrBI
Bme1580I BmgBI BmrI BpmI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgZI DraI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PfoI PspOMI PstI PvuII SacI
SapI SfcI SfoI SmaI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaLI AscI AsiSI AvrII BaeI BamHI
BbvCI BcgI BglI BglII BlpI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaWI BsiWI BspEI BsrBI BssHII
BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI DrdI
EciI EcoNI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SwaI XhoI ZraI